RT Journal Article SR Electronic(1) A1 ZOUHAIR, RACHID A1 DEFONTAINE, ALAIN A1 OLLIVIER, CECILE A1 CIMON, BERNARD A1 SYMOENS, FRANCOISE A1 HALLET, JEAN-NOEL A1 DEUNFF, JEAN A1 BOUCHARA, JEAN-PHILIPPEYR 2001 T1 Typing of Scedosporium apiospermum by multilocus enzyme electrophoresis and random amplification of polymorphic DNA JF Journal of Medical Microbiology, VO 50 IS 10 SP 925 OP 932 DO https://doi.org/10.1099/0022-1317-50-10-925 PB Microbiology Society, SN 1473-5644, AB The genetic diversity among epidemiologically unrelated strains of"the human pathogenic fungus Scedosporium apiospermum or its teleomorph, Pseudallescheria boydii, from different areas in Europe, was investigated by multilocus enzyme electrophoresis (MLEE) and random amplification of polymorphic DNA (RAPD). Fourteen enzyme activities were analysed by starch gel electrophoresis, corresponding to 27 polymorphic loci and 43 iso-enzymes. Among the enzymes studied, propionate esterase, carboxyl esterase, superoxide dismutase, carbonate dehydratase and malate dehydrogenase were the most polymorphic, allowing the classification of the strains into 6–11 groups each. Combination of the data obtained for the different enzyme activities studied allowed differentiation of the strains. Similarly, a high polymorphism was also revealed by each of the 20 RAPD primers tested, but no single primer was able to differentiate all the strains. The most efficient primers were GC70, UBC-701 and UBC-703, which revealed 17 distinct genotypes each, and combination of the results obtained with this three-primer set allowed complete discrimination of the strains. The dendrograms obtained from MLEE or RAPD by the unweighted pair-group method using arithmetic average cluster analysis did not reveal any clustering according to the geographic origin of the strains or their pathogenicity. , UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/0022-1317-50-10-925