1887

Abstract

Summary

On staining adenovirus-infected cells with phenanthrenequinone and with copper phthalocyanin-neutral red, characteristic structures in the form of rings and rosettes were observed. These structures could also be visualized by phase-contrast microscopy and by fluorescent antibody staining with adenovirus P antiserum. The cytochemical staining could be blocked by pre-treatment of the fixed cells with benzil, and the result suggested that the rings and rosettes were the sites of accumulation of arginine-rich basic proteins.

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1978-02-01
2024-12-09
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References

  1. Alfert M., Geschwind I. I. 1953; A selective staining method for the basic proteins of cell nuclei. Proceedings of the National Academy of Sciences of the United States of America 39:991
    [Google Scholar]
  2. Armstrong J. A., Pereira H. G. 1960; Significance of cytopathic effects observed during the growth of adenovirus. Experimental Cell Research 21:144
    [Google Scholar]
  3. Baker J. R. 1946; The histochemical recognition of lipine. Quarterly Journal of Microscopical Science 87:441
    [Google Scholar]
  4. Baker J.R. 1947; The histochemical recognition of certain guanidine derivatives. Quarterly Journal of Microscopical Science 88:115
    [Google Scholar]
  5. Eylar R. H, Thompson M. 1969; Allergic encephalomyelitis: the physico-chemical properties of the basic protein encaphalitogen from bovine spinal cord. Archives of Biochemistry and Biophysics 129:468
    [Google Scholar]
  6. Hayashi K, Russell W. C. 1968; A study of the development of adenovirus antigens by the immunoflourescent technique. Virology 34:470
    [Google Scholar]
  7. Horwitz M. S., Scharff M. D., Maizel J. V. JUN. 1969; Synthesis and assembly of adenovirus 2.1. Polypeptide synthesis, assembly of capsomeres and morphogenesis of the virion. Virology 39:682
    [Google Scholar]
  8. Itano H.A., Gottlieb A.J. 1963; Blocking of tryptic cleavage of arginyl bonds by the biochemical modification of the guanido group with benzil. Biochemical and Biophysical Research Communications 12:405
    [Google Scholar]
  9. Leibovitz A. 1963; The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. American Journal of Hygiene 78:173
    [Google Scholar]
  10. Levinthal J.D., Cerottini J. C., Ahmad-Zadeh C., Wicker R. 1967; The detection of intracellular adenovirus type 12 antigens by indirect immunoferritin technique. International Journal of Cancer 2:85
    [Google Scholar]
  11. Martinez-Paloma A., Le Buls A, Bernard W. 1967; Electron microscopy of adenovirus 12 replication. Journal of Virology 1:817
    [Google Scholar]
  12. Magun B. E, Kelly J. W. 1969; A new flourescent method with phenanthrenequinone for the histochemical demonstration of arginine residues in tissues. Journal of Histochemistry and Cytoehemistry I7:821
    [Google Scholar]
  13. Pearse A. G. E. 1968; Histoehemistry. Theoretical and Applied. , 3rd edition. Vol. 1: p. 426 London: J. D. A. Churchill Ltd;
    [Google Scholar]
  14. Rouse H. C., Schlesinger R. W. 1967; An arginine-dependent step in the maturation of type 2 adenovirus. Virology 33:513
    [Google Scholar]
  15. Russell W. C. 1971; The acid-soluble components of adenovirus and of adenovirus-infected cells. Journal of General Virology 11:65
    [Google Scholar]
  16. Russell W. C., Becker Y. 1968; A maturation factor for adenovirus. Virology 35:18
    [Google Scholar]
  17. Russell W. C., Hayashi K., Sanderson P. J., Pereira H.G. 1967a; Adenovirus antigens-A study of their properties and sequential development in infection. Journal of General Virology 1:495
    [Google Scholar]
  18. Russell W. C., Valentine R. C., Pereira H. G. 1967b; The effect of heat on the anatomy of the adenovirus. Journal of General Virology 1:509
    [Google Scholar]
  19. Russell W. C., Knight B. E. 1967; Evidence for a new antigen within the adenovirus capsid. Journal of General Virology 1:523
    [Google Scholar]
  20. Russell W. C., Laver W. G., Sanderson P. J. 1968; Internal components of adenovirus. Nature, London 219:1127
    [Google Scholar]
  21. Russell W. C, Mcintosh K., Skehel J. J. 1971; The preparation and properties of adenovirus cores. Journal of General Virology 11:35
    [Google Scholar]
  22. Schlesinger R. W. 1969 Adenoviruses: the nature of the virion and of controlling factors in productive or abortive infection and tumorogenesis. In Advances in Virus Research Vol. I4 p. 38 Ed. by Smith K. M., Lauffer M.A., Bang F. B. New York: Academic Press;
    [Google Scholar]
  23. Winters W. D., Russell W. C. 1971; Studies on the assembly of adenoviruses in vitro. Journalof General Virology 10:181
    [Google Scholar]
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