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Volume 65,
Issue 9,
1984
Volume 65, Issue 9, 1984
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Complete Nucleotide Sequence of a Satellite RNA of Tomato Black Ring Virus
More LessSUMMARYDouble-stranded cDNA copies of the satellite RNA of tomato black ring virus (strain S) have been cloned in pBR322 and sequenced. From these data the primary nucleotide sequence of the satellite RNA was deduced; it contains 1375 nucleotides and a poly(A) tail. Only one long open reading frame was found which codes for a protein of mol. wt. 47873. This sequence is flanked by a 5′-terminal sequence of 13 nucleotides and a 3′ non-coding region of 87 nucleotides, which contains a sequence that resembles signals for polyadenylation in other RNA molecules.
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Double-stranded RNA of Rice Gall Dwarf Virus
More LessSUMMARYNucleic acid extracted from purified particles of rice gall dwarf virus (RGDV) was identified as double-stranded RNA because it (i) was susceptible to RNase A in 0.1 × standard saline citrate (SSC) but not in 1 × SSC, (ii) was resistant to nucleae S1, (iii) showed no hyperchromicity in u.v. absorption after treatment with formaldehyde at 37 °C, (iv) showed a sharp thermal transition in u.v. absorption in 0.01 × SSC and (v) had a buoyant density of 1.596 g/ml in Cs2SO4. Electrophoresis in polyacrylamide slab gel revealed that RGDV RNA was composed of 12 segments with a total mol. wt. of about 16.9 × 106. Co-electrophoresis of RNA from RGDV and rice dwarf virus demonstrated that the electrophoretic mobilities of the seven larger segments from the two viruses were similar but that the five smaller segments differed in this respect. These results confirm that RGDV is a new virus and a third member of the genus Phytoreovirus.
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- Fungal
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Evidence for Sequence Heterogeneity among the Double-stranded RNA Segments of Penicillium chrysogenum Mycovirus
More LessSUMMARYWe have examined the dsRNA segments of the multipartite virus from Penicillium chrysogenum (ATCC 9480). A fourth RNA segment has been detected recently and, in the present work, we separated the four RNA segments and looked for possible homologies among them by thermal denaturation and electron microscopical heteroduplex studies. Differences were found between the differential melting profiles of the separated RNA segments, and no extensive regions of homology were detected among the various RNA segments by heteroduplex analyses. The results suggest that each RNA segment contains unique sequences. A comparison of the lengths of the RNA segments determined by electron microscopy with those determined by gel electrophoresis suggests that one of the segments has a distinct tertiary structure.
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High Frequency of Finding Double-stranded RNA in Naturally Occurring Isolates of Rhizoctonia solani
More LessSUMMARYForty-nine out of 50 field isolates of the basidiomycete Rhizoctonia solani were found to contain dsRNA. The presence of dsRNA appeared to have no consistent association with the degree of pathogenicity (virulence) of the isolates. These findings contradict previous reports correlating the presence of dsRNA with hypovirulence in R. solani.
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