Skip to content
1887

Journal of General Virology header logo - Volume 65, Issue 10, 1984

Volume 65, Issue 10

Volume 65, Issue 10, 1984

  • Plant

      • Serological Relationships and Genome Homologies among Geminiviruses

        https://doi.org/10.1099/0022-1317-65-10-1723
        SUMMARY

        In immunosorbent electron microscopy (ISEM) tests, strong relationships were detected between five whitefly-transmitted geminiviruses: African cassava mosaic (ACMV), bean golden mosaic, euphorbia mosaic, squash leaf curl and tomato golden mosaic. Among five leafhopper-transmitted geminiviruses, beet curly top and tobacco yellow dwarf viruses were distantly related but no relationship was detected between either chloris striate mosaic, maize streak or wheat dwarf viruses and any of the other four. No relationship was detected between any whitefly-transmitted and any leafhopper-transmitted virus. A similar pattern of relationships was found by spot hybridization experiments in which extracts from infected leaves were tested with probes for ACMV DNA-1 or DNA-2. Imperfect nucleotide sequence homologies were found between ACMV DNA-1, which contains the particle protein gene, and the DNA of five other whitefly-transmitted viruses: bean golden mosaic, tomato golden mosaic, tobacco leaf curl, tomato leaf curl and tomato yellow leaf curl, the last three of which are not sap-transmissible. Thus, relationships were established between sap-transmissible and sap non-transmissible geminiviruses. No homologies were detected with a full-length probe for ACMV DNA-2. Extracts from plants infected with three leafhopper-transmitted viruses (beet curly top, maize streak and wheat dwarf) did not react with probes for ACMV DNA-1 or DNA-2. Because each of the leafhopper-transmitted geminiviruses has a different vector species whereas the whitefly-transmitted geminiviruses all have the same vector, , the genome homologies and antigenic relationships detected among members of the group could be explained if their coat proteins have a key role in transmission by vectors.

    • Nucleotide Sequence Homologies among RNA Species of Strains of Tomato Black Ring Virus and Other Nepoviruses

      https://doi.org/10.1099/0022-1317-65-10-1731
      SUMMARY

      Nucleotide sequences of the genome RNA species of four strains of tomato black ring virus (TBRV), two each from the Scottish and German serotypes, were compared by hybridization with complementary DNA copies. Within each serotype 90% or more homology was observed between the corresponding RNA species of each pair of strains. Between serotypes greater differences were found, with 30 to 40% of RNA-1 sequences and only about 10% of RNA-2 sequences being common to all four strains. Hybridization reactions between RNA species and complementary DNA preparations representing different serotypes proceeded more slowly, and the hybrids melted at lower temperatures than those between homologous combinations, indicating that the apparently conserved sequences are similar, but not identical, in the two serotypes. Satellite RNA species from two TBRV strains in different serotypes had only about a quarter of their sequences in common. Limited nucleotide sequence homology was found between the genome RNA species of TBRV and those of the serologically distantly related grapevine chrome mosaic, cacao necrosis and artichoke Italian latent viruses, but not between TBRV RNA species and those of seven serologically unrelated nepoviruses.

Top
◄ 1 2
20 | 50 | 100 per page

Volumes and issues

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error