- Volume 64, Issue 6, 1983
Volume 64, Issue 6, 1983
- Animal
-
-
-
The Avirulent A7 Strain of Semliki Forest Virus Has Reduced Cytopathogenicity for Neuroblastoma Cells Compared to the Virulent L10 Strain
More LessSUMMARYMolecular and host range properties of the virulent L10 strain of Semliki Forest virus were compared to those of the avirulent A7 strain. No difference could be detected between the two strains in adsorption, nucleocapsid synthesis, protein synthesis, ratio of 42S:26S RNA, particle infectivity, interferon induction and susceptibility, or defective interfering particle production. A7 showed lower total RNA synthesis than L10 in BHK, G26-24 (oligodendroglioma) and C1300 (neuroblastoma) cells. Cytopathogenicity of A7 was reduced compared to L10 in C1300 cells but not in G26-24 cells. It is concluded that the avirulent A7 strain has similar host range and molecular properties to the neurovirulence mutants M9 and M136, and that demyelination may be produced by a similar mechanism.
-
-
-
-
Neutralizing and Non-neutralizing Monoclonal Antibodies to the E2 Glycoprotein of Semliki Forest Virus Can Protect Mice from Lethal Encephalitis
More LessSUMMARYTwo monoclonal antibodies (UM 4.2 and UM 5.1) directed against the glycoprotein E2 of Semliki Forest virus (SFV) are described; both belong to the IgG2a isotype but are of different idiotype. Analysis employing isoelectric focusing resulted in different focusing patterns for both monoclonals (UM 4.2, pI 8; UM 5.1, pI 7.2). They further differed in their ability to neutralize virus. The UM 4.2 antibodies were inactive in neutralization, while the UM 5.1 antibodies exceeded conventional mouse hyperimmune serum in this respect. Both monoclonal antibodies, however, were able to protect mice passively from a lethal infection with SFV. Based on the amount of protein, the UM 5.1 antibodies were 100-fold more effective than the UM 4.2 antibodies in mouse protection tests.
-
-
-
Purification and Molecular Weight Determination of Measles Virus Genomic RNA
More LessSUMMARYA purification procedure for genomic measles virus RNA, free of contaminating smaller RNA and of DNA, is described. Viral nucleocapsids were prepared from MA160 cells infected in spinner cultures with measles virus (Edmonston strain). Nucleic acid was extracted, treated with DNase and RNA sedimenting at about 50S in sucrose gradients was isolated. This method yielded 0.5 to 1.5 µg of genomic RNA per litre of culture. A molecular weight of 4.5 × 106 was determined by gel electrophoresis under fully denaturing conditions.
-
-
-
Generation and Characterization of a Non-defective Interfering Particle of Vesicular Stomatitis Virus: Homotypic and Heterotypic Interference
More LessSUMMARYSerial undiluted passages of vesicular stomatitis virus in African green monkey kidney (CV-1) cells resulted in the generation of a small-plaque-forming mutant. This mutant has been characterized as a non-defective particle with homotypic and heterotypic interference properties.
-
-
-
Biochemical Characterization of Eel Virus European
More LessSUMMARYThe polypeptide and RNA compositions of purified virions of eel virus European were compared to those of three strains (VR-299, SP and AB) of infectious pancreatic necrosis virus (IPNV) isolated from trout. All three IPNV strains could be distinguished by the relative mobilities of either the virion polypeptides or the double-stranded RNA genome segments. The eel virus had a similar polypeptide profile to strain AB IPNV but differences between the two viruses in the migration of genome segments indicated each was unique.
-
- Plant
-
-
-
Interference in Infections of Tobacco Protoplasts with Two Bromoviruses
More LessSUMMARYFreshly prepared tobacco mesophyll protoplasts behave as if they contain two classes, one of which is resistant to infection but the other is susceptible and can readily be doubly infected with brome mosaic virus (BMV) and cowpea chlorotic mottle virus (CCMV). BMV dominates in all double infections and although both types of virus particles are produced, only those of BMV are infectious. The defective CCMV particles contain normal amounts of RNA but only RNA 3 could be detected in them. No CCMV RNA 2 could be detected in doubly infected protoplasts, showing that there was only partial replication of the genome of CCMV. The proteins encoded by CCMV RNA 3 were produced in mixed infections. It is possible that BMV may exploit the gene products of CCMV while suppressing synthesis of infectious CCMV particles. There was no evidence that BMV RNA was encapsidated in CCMV coat protein.
-
-
Volumes and issues
-
Volume 105 (2024)
-
Volume 104 (2023)
-
Volume 103 (2022)
-
Volume 102 (2021)
-
Volume 101 (2020)
-
Volume 100 (2019)
-
Volume 99 (2018)
-
Volume 98 (2017)
-
Volume 97 (2016)
-
Volume 96 (2015)
-
Volume 95 (2014)
-
Volume 94 (2013)
-
Volume 93 (2012)
-
Volume 92 (2011)
-
Volume 91 (2010)
-
Volume 90 (2009)
-
Volume 89 (2008)
-
Volume 88 (2007)
-
Volume 87 (2006)
-
Volume 86 (2005)
-
Volume 85 (2004)
-
Volume 84 (2003)
-
Volume 83 (2002)
-
Volume 82 (2001)
-
Volume 81 (2000)
-
Volume 80 (1999)
-
Volume 79 (1998)
-
Volume 78 (1997)
-
Volume 77 (1996)
-
Volume 76 (1995)
-
Volume 75 (1994)
-
Volume 74 (1993)
-
Volume 73 (1992)
-
Volume 72 (1991)
-
Volume 71 (1990)
-
Volume 70 (1989)
-
Volume 69 (1988)
-
Volume 68 (1987)
-
Volume 67 (1986)
-
Volume 66 (1985)
-
Volume 65 (1984)
-
Volume 64 (1983)
-
Volume 63 (1982)
-
Volume 62 (1982)
-
Volume 61 (1982)
-
Volume 60 (1982)
-
Volume 59 (1982)
-
Volume 58 (1982)
-
Volume 57 (1981)
-
Volume 56 (1981)
-
Volume 55 (1981)
-
Volume 54 (1981)
-
Volume 53 (1981)
-
Volume 52 (1981)
-
Volume 51 (1980)
-
Volume 50 (1980)
-
Volume 49 (1980)
-
Volume 48 (1980)
-
Volume 47 (1980)
-
Volume 46 (1980)
-
Volume 45 (1979)
-
Volume 44 (1979)
-
Volume 43 (1979)
-
Volume 42 (1979)
-
Volume 41 (1978)
-
Volume 40 (1978)
-
Volume 39 (1978)
-
Volume 38 (1978)
-
Volume 37 (1977)
-
Volume 36 (1977)
-
Volume 35 (1977)
-
Volume 34 (1977)
-
Volume 33 (1976)
-
Volume 32 (1976)
-
Volume 31 (1976)
-
Volume 30 (1976)
-
Volume 29 (1975)
-
Volume 28 (1975)
-
Volume 27 (1975)
-
Volume 26 (1975)
-
Volume 25 (1974)
-
Volume 24 (1974)
-
Volume 23 (1974)
-
Volume 22 (1974)
-
Volume 21 (1973)
-
Volume 20 (1973)
-
Volume 19 (1973)
-
Volume 18 (1973)
-
Volume 17 (1972)
-
Volume 16 (1972)
-
Volume 15 (1972)
-
Volume 14 (1972)
-
Volume 13 (1971)
-
Volume 12 (1971)
-
Volume 11 (1971)
-
Volume 10 (1971)
-
Volume 9 (1970)
-
Volume 8 (1970)
-
Volume 7 (1970)
-
Volume 6 (1970)
-
Volume 5 (1969)
-
Volume 4 (1969)
-
Volume 3 (1968)
-
Volume 2 (1968)
-
Volume 1 (1967)