1887

Journal of General Virology header logo - Volume 58, Issue 1, 1982

Volume 58, Issue 1

Volume 58, Issue 1, 1982

  • Animal

      • Intracellular Nucleocapsid RNA of Mumps Virus

        https://doi.org/10.1099/0022-1317-58-1-205
        SUMMARY

        Intracellular nucleocapsids, virtually free of host proteins and nucleic acids, were purified from cells acutely infected with several strains of mumps virus. Viral RNA obtained from the nucleocapsids was predominantly a single 50S species, with 28S RNA occurring as a secondary peak in the RNA profile. Nucleocapsids yielded approx. 9 µg 50S RNA per 10 infected cells. The 50S RNA was partially self-complementary, indicating that mumps viral nucleocapsids include full-length plus (+) sense antigenomes. Self-annealing and competition hybridization assays with 50S RNA suggest that 28S RNA includes duplexes consisting of full-length (+) and minus (-) sense 50S RNA. Properties of the viral RNA did not vary with the method of RNA isolation, time post-infection at which nucleocapsids were harvested, nor the strain of mumps virus.

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