- Volume 41, Issue 3, 1978
Volume 41, Issue 3, 1978
- Articles
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Protease Activation of Sendai Virus Infectivity; Studies in Non-Permissive and Permissive Cells
More LessSUMMARYNon-infectious virus particles are produced by BSC-1 cells after infection with Sendai virus. Trypsin treatment of these particles activates their infectivity. The studies reported here show that such non-infectious virus particles adsorb normally to cells but cannot initiate infection even after very long adsorption periods. Secondary Rhesus monkey kidney cells support the growth of Sendai virus but cannot activate the infectivity of virus grown in BSC-1 cells. The significance of these results is discussed.
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Early Events in the Infection of Tobacco with Alfalfa Mosaic Virus
More LessSUMMARYAt 23 °C, infections with alfalfa mosaic virus can be initiated with the three genomic RNA species in combination either with coat protein or with the messenger for coat protein (RNA 4). At 30 °C the combination of the three genomic RNA species with coat protein is still infectious to tobacco, whereas the infectivity of the combination with RNA 4 is almost nil. The combination of the four RNA species is infectious to bean at both temperatures. Tobacco leaf discs inoculated with the four AMV RNA species were incubated for short periods at 30 °C followed by 4 days at 23 °C. Infectivity could only be recovered when the shift-down occurred within 10 min after inoculation. Longer exposure to 30 °C probably leads to the degradation of one or more of the AMV RNA species. Translation of RNA 4 into coat protein at 23 °C in the inoculated cells presumably will render the RNA infection resistant to 30 °C. To measure the minimum time required for the penetration and translation of RNA 4, tobacco leaf discs inoculated with the four AMV RNA species were incubated for different periods at 23 °C and then for 4 days at 30 °C. From the results it was estimated that 15 to 30 min is sufficient for the penetration and translation of RNA 4.
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Coat Protein is Required for Infection of Cowpea Protoplasts with Alfalfa Mosaic Virus
More LessSUMMARYInfection of cowpea protoplasts with alfalfa mosaic virus RNA was achieved by sedimenting protoplasts from 0·5 m-mannitol and resuspending them in 0·7 m-mannitol containing 0·1 m-potassium phosphate, pH 6·0, and 100 μg/ml RNA. The osmotic shift-up employed during inoculation substantially enhanced the infection by virus RNA as well as by virus nucleoprotein.
As with intact plants, a mixture of the virus genomic RNA species (RNA 1, 2 and 3) was infectious to protoplasts only when supplemented either with virus coat protein or with the messenger RNA for coat protein (RNA 4).
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