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Volume 41,
Issue 2,
1978
Volume 41, Issue 2, 1978
- Articles
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Effect of Interferon on the Cell Cycle of BALB/c 3T3 Cells
More LessSUMMARYThe effect of interferon on the exponential growth phase of BALB/c 3T3 cells was studied. Although interferon reduced the growth rate and the proportion of cells in both the M and S phases (mitotic index and labelling index), there were no appreciable differences in the duration of these phases between control and interferon-treated cells. Moreover, the shape of the first peak of the fraction of labelled mitoses (FLM) curve was not altered by treatment with interferon, which indicates that the duration of the S and G2 phases was not affected. However, the height of the second peak of the curve in interferon-treated cells was extremely reduced as compared to control culture. These results are compatible with the idea that the suppressive effect of interferon is exerted mainly in the G1 phase (A-state) of the cell cycle of BALB/c 3T3 cells.
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Ultrastructural Study of Virus-like Particles in Chinese Hamster Lung Cells
More LessSUMMARYVirus-like particles were found in the E36 cells. One type, associated with centrioles, consisted of two concentric shells with a diam. of 50 to 60 nm. Some of these particles were seen budding through the plasma membrane giving rise to a free immature particle showing two concentric shells and an outer envelope. Concentration of the inner shell into a nucleoid results in a mature particle characterized by a nucleoid, mainly eccentrically located, surrounded by an intermediate layer and wrapped in an envelope. The diameter of the mature and immature particles was 75 to 85 nm. The morphogenesis of this virus-like particle resembles that of the oncoviruses.
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Effect of Interferon on Transcription and Translation of Vesicular Stomatitis Virus in Human and Simian Cell Cultures
More LessSUMMARYThe effect of interferons on vesicular stomatitis virus (VSV) primary transcription, amplified RNA synthesis [i.e. the sum of primary transcription RNA replication (leading to [–] RNA) and secondary transcription (leading to [+] RNA) and virus protein synthesis were studied. In a human cell line, both human and simian interferons inhibited the initiation of primary transcription and amplified RNA synthesis. In contrast, in a simian cell line tested similarly, the initiation of these activities was not affected, though they decreased as the infection progressed. Nevertheless, virus protein synthesis was completely inhibited. These results demonstrate that the action of interferon on virus transcription and/or translation may depend more on the host cell than on the particular interferon used.
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Synthesis of Herpes Simplex Virus DNA in Preparations of Chromatin from Infected Cell Nuclei
More LessSummaryChromatin prepared from cells infected with Herpes simplex virus type 1 or type 2 can synthesize both virus and cell DNA in vitro. The rate of synthesis is comparable to that of isolated whole nuclei. Incorporation is limited, and both cell and virus DNA synthesis are sensitive to the presence of virus-specific antiserum and phosphonoacetate. In chromatin from cells infected with a phosphonoacetate resistant virus mutant, both types of DNA synthesis are resistant to the presence of the inhibitor.
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Characterization of Adenovirus Type 12 Tumour Antigen Produced in Chick Fibroblasts
More LessSUMMARYTumour (T) antigen was characterized in non-permissive chick fibroblasts and permissive HEp-2 cells infected with Ad12 either in the presence or in the absence of cytosine arabinoside. Antiserum against T antigen specifically immunoprecipitated two polypeptides of apparent mol. wt. 50000 and 11000.
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Effect of Trypsin Treatment on the Antigenic Characteristics of Plaque Variants of Type O1 and Type Asia-1 Foot-and-Mouth Disease Viruses
More LessSummaryAntigenic differences were demonstrated between the large and small plaque variants of both types O1 and Asia-1 foot-and-mouth disease viruses. Treatment of the large and small plaque variants of the viruses with trypsin essentially abolished the observed antigenic differences. Thus, these plaque variants have antigenically different trypsin-sensitive determinants that may influence their immunogenicity and infection capabilities.
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Presence of a Covalently Linked Protein on Calicivirus RNA
More LessSummaryThe infective RNA of the calicivirus, vesicular exanthema virus, has been shown to contain a protein which is apparently linked to the RNA by a covalent bond. The protein remained bound to the RNA after boiling with SDS-mercaptoethanol-urea or treating with formamide-dimethylsulphoxide but was removed by incubating with proteinase K. The mol. wt. of the protein was estimated to be about 10 × 103 by electrophoresis in highly cross-linked polyacrylamide gels. The infectivity of the RNA was destroyed by removal of the protein with proteinase K.
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