Journal of General Virology - Volume 12, Issue 2, 1971
Volume 12, Issue 2, 1971
- Articles
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Studies with Avian Influenza A Viruses: Serological Relations of the Haemagglutinin and Neuraminidase Antigens of Ten Virus Isolates
More LessSummaryTen strains of avian influenza A viruses, naturally pathogenic for birds and grown in chick embryo allantois were compared by haemagglutination-inhibition and neuraminidase-inhibition tests, using chicken antisera. The viruses segregated into different cross-reaction groups in the two tests, emphasizing the need to include an assessment of neuraminidase antigens in the taxonomy of influenza A viruses. Four major groups of viruses were observed in the haemagglutination-inhibition test but several very low titre cross-reactions were constantly found outside these groups. Some of these reactions, not all of which were two-way, corresponded to anti-neuraminidase cross-reactions. The results of neuraminidase-inhibition tests, using a largely automated technique and a human serum fraction as substrate, confirmed our previous results obtained with a smaller substrate. The virus neuraminidases could be separated into five separate antigen groups, without cross-reactions between the groups.
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Studies with Avian Influenza A Viruses: Cross Protection Experiments in Chickens
More LessSummaryPrevious work on the serological relationships of avian influenza A viruses provided an opportunity to assess the protective effects of anti-neuraminidase anti-body in a single species system. Three viruses naturally lethal for the chicken (A/dutch, A/turkey/england/63 and A/tern/south africa/61) were selected so that two (A/dutch and A/turkey/england/63) had an antigenically similar haemagglutinin, two (A/turkey/england/63 and A/tern/southafrica/61) had a similar neuraminidase and two (A/dutch and A/tern/south africa/61) were unrelated by surface antigens. Each virus was used to immunize a group of 7-week-old chickens using one dose of killed virus followed by one dose of live virus. Each group was then divided into three subgroups and each subgroup challenged with one of the viruses. The results of cross-challenge with live virus were assessed objectively on mortality. (1) Immunization with any of the viruses protected the birds completely against the homologous virus. (2) Immunization protected the birds completely against a heterologous virus possessing a similar haemagglutinin. (3) Immunization gave partial protection against a heterologous virus possessing a similar neuraminidase and this protection was significantly greater (P = 0.01) than unimmunized controls when birds immunized with A/turkey/england/63 virus were challenged with A/tern/south africa/61. In birds immunized with A/tern/south africa/61 and challenged with A/turkey/england/63 the protection was approaching significance (P < 0.1 > 0.05). The results of testing the sera from the immunized birds confirmed the seriological relationships of the viruses.
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Mechanism of Carcinogenesis by RNA Tumour Viruses: The RNA- and DNA-dependent DNA Polymerase Activities of Feline Sarcoma Virus
K. Fujinaga and M. GreenSummaryIn the absence of added template, the DNA polymerase of the feline sarcoma virus particle appeared to exhibit two-phase kinetics—a rapid reaction for about 4 min., followed by a slower reaction. Equilibrium sedimentation in Cs2SO4 density gradients showed the formation of RNA-DNA complexes within 12 sec., followed by the formation of DNA free of an RNA template. Virtually all of the DNA product formed at 4 min. and later was free of RNA template. About half of the 60 min. DNA product was double-stranded, since it was resistant to digestion by exonuclease I and eluted from hydroxyapatite columns at the salt concentration characteristic for duplex DNA. After denaturation, the 15, 30 and 60 min. DNA product sedimented at 6s and formed a broad band in CsCl gradients with an average buoyant density of about 1.725. Calf thymus DNA stimulated DNA polymerase activity of detergent-treated virus particle about 10- to 20-fold, indicating the presence of DNA-dependent as well as RNA-dependent DNA polymerase activity within the virus particle.
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Alterations in the Susceptibility of Cultured Mouse Cells to Transformation by Murine Sarcoma Virus (harvey)
More LessSummaryDuring serial passage of mouse embryo fibroblasts in vitro it was found that an established line of cells was more susceptible to murine sarcoma virus transformation than early passages of the cells from which it was derived. An explanation can be made in terms of the growth rates of the cells involved, although primary cells were more susceptible to murine sarcoma virus than would be expected from their rate of growth. Non-dividing (amitotic) cells were not susceptible to murine sarcoma virus transformation.
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The Morphogenesis and Classification of Bovine Syncytial Virus
More LessSummaryBovine syncytial virus was found to replicate in BHK 21 cells. A thin-section study of the morphogenesis of the virus in BHK 21 cells indicated that virus precursor is formed in the nucleus and, in some cells at least, is liberated into the cytoplasm by breakdown of the nuclear envelope. Internal components were found in the nucleus and the cytoplasm and also embedded in the chromosomes of dividing nuclei. Virus internal components gained their envelopes by budding through one of a variety of cytoplasmic membranes, including the plasma membrane. Some stages of the morphogenesis of bovine syncytial virus in BESp cells, and of a type 1 simian foamy virus in BHK 21 cells, are also illustrated. It is concluded that bovine syncytial virus, a feline agent and simian foamy agents all belong to the same virus group.
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Reovirus: Analysis of Proteins from Released and Cell-associated Virus
More LessSummaryThe proteins of purified preparations of reovirus type 3 have been examined using polyacrylamide gel electrophoresis. Preparations of cell-associated virus grown with foetal calf serum show three major peaks, λ, µ, σ, and several minor peaks. Preparations of released virus grown without foetal calf serum show only the three major peaks, suggesting that the minor peaks are not virus proteins.
Analysis of preparations of released virus labelled with both [14C]lysine and [3H] leucine shows that each of three major peaks contains more than one polypeptide chain. The molecular weights of the polypeptides are: 140,000 to 150,000 for the λ group, 75,000 to 85,000 for µ and 36,000 to 44,000 for σ, as determined by migration in 5% sodium dodecyl sulphate-polyacrylamide gels. There are at least eight polypeptide chains distributed as two in peak λ, three in peak µ, and three in peak σ.
Top component particles isolated during purification of virus show the same three protein peaks as demonstrated in complete virus. Virus cores produced by trypsin or chymotrypsin treatment of the virus lack µ-group polypeptides and some σ polypeptides.
Radioactive glucosamine and fucose are not incorporated into any of the three groups of virus particle proteins during the growth cycle. The content of amino sugar or methylpentose in purified virus is less than 0.1% and that of neutral carbohydrates less than 1%. These results indicate that purified preparations of reovirus type 3 contain little if any carbohydrate. Such purified preparations agglutinate ox red blood cells.
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Comparison of Interferon Induction in Mice by Purified Penicillium chrysogenum Virus and Derived Double-stranded RNA
More LessSummaryA virus preparation, obtained from the mycelium of Penicillium chrysogenum, strain Q176, was purified by isopycnic centrifugation on linear gradients of potassium tartrate. Double-stranded RNA, prepared from the virus particles, revealed three components on electrophoresis on polyacrylamide gels.
The serum interferon levels in mice after intravenous injection of free virus RNA were higher and earlier than for purified virus particles, containing an equivalent amount of RNA. After reaching their maxima, the interferon activities declined at similar rates to very low levels after 45 hr for free RNA and 39 hr for virus particles.
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The Virulence of Original and Derived Strains of Semliki Forest Virus for Mice, Guinea-pigs and Rabbits
More LessSummaryThe virulence of several early and serologically indistinguishable strains of the four original isolations of Semliki Forest virus was defined quantitatively through the response of mice, guinea-pigs and rabbits to intraperitoneal, respiratory and intracerebral administration of virus. There was a clear gradient of virulence against a corresponding gradient in the efficiency of the hosts' protective responses. When the responsiveness of the host was critically balanced against the virulence of the invading virus a transitional state developed in which the groups of infected test animals showed a dual response in which some died and some were protected. This may have been due to the presence in some virus samples of subpopulations of extreme virulence and avirulence. Regardless of the ultimate response being death, incapacitation or benign protection, the efficiency of initial invasion and replication of virus was uniformly high for virus strains of extreme virulence or avirulence.
We have provided a specification of the virulence of virus and of the efficiency of the hosts' protective responses which may be of value in the standardization of other studies on virus heterogeneity and the induction of the immune state.
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Electron Microscopic Studies of Coronavirus
More LessSummaryElectron-microscopic studies of the morphology and development of a coronavirus (linder strain), isolated in human foetal diploid lung cells from a case of upper respiratory illness, revealed virus particles of diameter 80 to 160 nm. They were initially observed 12 hr after infection. Extracellular and intracytoplasmic virus concentration increased sharply at 18 hr and reached a maximum at 24 hr. The number of particles decreased slightly at 48 hr and by 72 hr many cells were lysed. The particles formed by budding into the cisternae of the endoplasmic reticulum or into an inclusion composed of tubular structures resembling part of the complete virus particle. There were cytoplasmic inclusions of dense particles within a granular matrix and surrounded by a double membrane. The release of particles by lysis is illustrated. Extracellular virus was specifically tagged with ferritin-labelled antibody.
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An A Particle, Probably of Bovine Origin, Detected in BHK21 Cells Infected with Bovine Syncytial Virus
More LessTo initiate an investigation of bovine syncytial virus, two cultures of bovine embryonic spleen cells were obtained from Dr M. J. van der Maaten. One of these cultures was already inoculated with bovine syncytial virus. To propagate the virus, inoculated and uninoculated cultures of bovine embryonic spleen cells were mixed. The cells were initially grown in Eagle's medium with 20% foetal calf serum, but this was changed after a few passages to Eagle's medium plus 10% tryptose phosphate broth and 10% foetal calf serum (ETC). After several passages the spleen cells ceased to grow well, so an attempt was made to propagate the virus in BHK 21 cells. Infected spleen-cell cultures were mixed in ETC medium with BHK 21 cells and syncytia developed in the mixed cultures. The cells were subcultured at 2- or 3-day intervals, and after 3 weeks the cytopathic effect was widespread.
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New Evidence on the Structure of Nepoviruses
More LessThe nepoviruses (Harrison et al. 1971) are a group of nematode-transmitted plant viruses that have isometric particles with slightly angular outlines and diameters of 28 to 30 nm. Purified preparations of these viruses typically contain particles of three types, differing in RNA content (Stace-Smith, Reichmann & Wright, 1965; Diener & Schneider, 1966). The particle structure of nepoviruses has been little studied but, using evidence obtained by electron microscopy of negatively stained preparations, it was proposed that the protein shells of two nepoviruses, tobacco ringspot (Chambers, Francki & Randles, 1965) and arabis mosaic (Agrawal, 1967), are composed of 42 morphological subunits. This work did not, however, take into account all other possibilities and our results, described below, show that the particles cannot be built of 42 morphological subunits.
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Differentiation between Type 1 and Type 2 Strains of Herpes Simplex Virus by an Indirect Immunofluorescent Technique
More LessThe cytoplasmic membranes of cells infected with herpes simplex virus develop altered antigenic specificities (O'Dea & Dineen, 1957; Roizman & Spring, 1967). Strain-specific differences in these surface antigens have been reported (Roizman & Roane, 1963; Peterknecht, Bitter-Suerman & Falke, 1968). In the course of a study of antigenic specificities in BHK cells infected with several herpes simplex viruses (cf. Watson et al. 1966), we have found that the use of appropriately absorbed antisera permits the detection of type-specific membrane antigens. This forms the basis of the simple and rapid immunofluorescent method for typing strains of herpes simplex virus which we report in this note.
Exploratory work was done with two established type 1 strains (hfem which originated from the Rockefeller strain hf and wal, a recurrent oral isolate) and two established type 2 strains (lov, kindly given us by Dr A. J. Nahmias and bry, a recurrent genital isolate).
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Effects of Cycloheximide on the Accumulation of Tobacco Rattle Virus in Leaf Discs of Nicotiana clevelandii
More LessThe longer of the two types of particle of the cam strain of tobacco rattle virus is associated with mitochondria in the cells of Nicotiana clevelandii Gray leaves (Harrison & Roberts, 1968), and the RNA-producing defective isolate, cam/df, causes gross structural abnormalities in mitochondria (Harrison, Stefanac & Roberts, 1970). Thus mitochondria may play a special role in the synthesis or assembly of tobacco rattle virus.
In plant tissue, chloramphenicol is reputed to inhibit protein synthesis by chloroplast, and possibly by mitochondrial but not by cytoplasmic ribosomes, whereas cycloheximide is claimed to inhibit protein synthesis by cytoplasmic but not chloroplast ribosomes (Ellis, 1969; Boulter, 1970). We have therefore tested the effects of these and some other compounds on the accumulation of tobacco rattle virus, to see whether the results would shed any fresh light on the possible role of organelles in the replication of the virus.
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Further Studies on a Purification Procedure for Encephalomyocarditis Virus
More LessThe procedure used in this laboratory for the purification of encephalomyocarditis (EMC) virus has been described by Burness (1969a). Contamination with host cell material was shown to be absent by the lack of radioactivity in purified virus when homogenates of uninfected host cells containing protein and nucleic acid labelled with [3H]amino acids and 32P, respectively, were added to the crude virus before purification (Burness, 1969a). Although several methods were employed to check the purity of the product, contamination with proteins unique to infected cells, such as non-structural virus proteins was not excluded. We describe here a procedure to investigate and virtually exclude such possible contamination, by using EMC virus variants separable from one another by calcium phosphate chromatography.
In the original description of the procedure it was suggested that ribosomes would be the most likely subcellular contaminants of picornavirus preparations since both kinds of particles have similar biophysical characteristics (Burness, 1969a).
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Interferon Clearance Rate Decreased after Repeated Injections
More LessA rapid rate of clearance of circulating interferon has been observed in mice (Baron et al. 1966; Gresser et al. 1967; Subrahmanyan & Mims, 1966) and in rabbits (Ho & Postic, 1967) after a single injection of interferon. However, in a recent study (De Clercq, Nuwer & Merigan, 1970) the rate seemed to be slower after multiple intravenous injections of interferon. These observations are extended in this paper.
Interferon was injected into the lateral tail vein of 2- to 4-week-old randomly bred Swiss-Webster albino mice. Animals were then bled at different times from the right retro-orbital plexus, and sera from two mice were pooled for each assay sample.
Mouse interferon was produced in L-929 cells as described elsewhere (Hanna, Merigan & Jawetz, 1966) with the Cassell 73-t strain of Newcastle disease virus (NDV). After inactivation of residual NDV at pH 2 for 5 days, the tissue culture fluid was concentrated tenfold by pressure dialysis in a pressure cell (Amicon Corporation, Cambridge, Massachusetts) employing a UM-10 filter.
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Differences in the Properties of Thymidine Kinase Produced in Cells Infected with Type 1 and Type 2 Herpes Virus
More LessThymidine kinase activity is known to increase in BHK 21 cells infected with herpes simplex virus type 1 (Klemperer et al. 1967). Moreover, there is now evidence showing that this is a virus-specified enzyme (Klemperer et al. 1967; Buchan & Watson 1969; Buchan et al. 1970). We now report a similar increase in thymidine kinase activity in BHK 21 cells infected with herpes simplex virus type 2. The pH optima are the same for both enzymes. The growth kinetics are similar and the type 2 virus always induces at least as much enzyme activity as the type 1 and usually up to twice as much. There are striking differences in the stability and serological specificity of the two enzymes.
Infected BHK cell extracts were prepared and the thymidine kinase activity measured by the method of Klemperer et al. (1967). Briefly, BHK cells were infected with herpes simplex virus at a multiplicity of infection of 10 to 20 p.f.u./cell.
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