- Volume 12, Issue 1, 1971

Electrophoretic analyses of the polypeptide components of adenovirus indicate that both the hexon and the penton base are degraded during storage of either purified virus preparations or isolated antigens. These two protein components are also degraded by trypsin and the products of tryptic digestion of native hexons were examined in detail. They were shown to consist of six polypeptide species which may result from tryptic action at three distinct sites on the hexon polypeptide.

Both spontaneously degraded and trypsinized hexon and penton base proteins retained their morphological and antigenic characteristics.

To evaluate the latex test for detecting distant serological relationships among plant viruses, latex coated first with antibody and then with virus was used to determine antiserum titres. In tests with latex coated with elongated viruses of the potato virus X group and with isometric viruses of the turnip yellow mosaic virus group the homologous titres of antisera were always much higher than in slide precipitin or agar diffusion tests. Usually, the heterologous titres were also increased and more heterologous reactions were found, especially when the pure γ-globulin fraction was used instead of unfractionated sera, even though this γ-globulin fraction contained relatively few antibodies.

With both groups of viruses, the results corroborate previous observations made using conventional serological methods. In addition, turnip yellow mosaic virus was shown for the first time to be distantly serologically related to Andean potato latent and belladonna mottle viruses. The latex test may offer improved opportunities to detect distant serological relationships among members of other groups of viruses.

Mycophage PS 1, produced by the mould Penicillium stoloniferum, was concentrated and purified by zonal centrifugation or by a combination of isopropanol precipitation and zonal centrifugation. Continuous flow isopycnic banding was accomplished using the K-II zonal centrifuge. Isopropanol precipitates of fermentation broths were further concentrated by continuous flow isopycnic banding in the B-IX rotor followed by rate zonal separation and a second isopycnic banding in the B-XV rotor. Mycophage PS 1 was a potent inducer of interferon. Double-stranded RNA isolated from both the virus-like particle and from the rate zonal gradient also induced interferon. The characters of the double-stranded RNA from both sources were identical.

Infection of primary human embryo kidney cells with adenovirus types 2, 7, 12, 18 and 31, at a multiplicity of infection of 2, resulted in the induction of chromosome aberrations in metaphases examined 24 hr after infection. The aberrations induced by types 2 and 7 were randomly distributed over the karyotype, but types 12 and 31, and to a lesser extent type 18, all induced non-random effects. Both type 12 and type 31 produced chromatid and isochromatid gaps and breaks at a specific site on chromosome no. 17.