1887

Abstract

Insect responses that are specific for virus infection have been investigated using the genetically tractable . Most studies focus on interactions with C virus (DCV), which is a member of the family . DCV is a non-enveloped, =3 icosahedral virus with a positive-sense RNA genome. It was demonstrated recently that several genes controlled by the Jak-STAT pathway are specifically upregulated upon DCV infection. To investigate the virus factors that induce these responses, we used the Jak-STAT regulated genes as reporter genes. Challenge of flies with non-infectious DCV particles or double-stranded RNA did not stimulate significant upregulation of the antiviral response genes. In addition, there was no difference in reporter gene upregulation between challenged with three different strains of DCV. This suggests that upregulation of these genes may require virus replication and may involve the non-structural proteins of DCV.

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2008-06-01
2019-11-17
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References

  1. Cherry, S. & Perrimon, N. ( 2004; ). Entry is a rate-limiting step for viral infection in a Drosophila melanogaster model of pathogenesis. Nat Immunol 5, 81–87.
    [Google Scholar]
  2. Cherry, S. & Silverman, N. ( 2006; ). Host–pathogen interactions in drosophila: new tricks from an old friend. Nat Immunol 7, 911–917.[CrossRef]
    [Google Scholar]
  3. Christian, P. D. ( 1992; ). A simple vacuum dot-blot hybridisation assay for the detection of Drosophila A and C viruses in single Drosophila. J Virol Methods 38, 153–165.[CrossRef]
    [Google Scholar]
  4. Dostert, C., Jouanguy, E., Irving, P., Troxler, L., Galiana-Arnoux, D., Hetru, C., Hoffmann, J. A. & Imler, J. L. ( 2005; ). The Jak-STAT signaling pathway is required but not sufficient for the antiviral response of Drosophila. Nat Immunol 6, 946–953.[CrossRef]
    [Google Scholar]
  5. Dzitoyeva, S., Dimitrijevic, N. & Manev, H. ( 2001; ). Intra-abdominal injection of double-stranded RNA into anesthetized adult Drosophila triggers RNA interference in the central nervous system. Mol Psychiatry 6, 665–670.[CrossRef]
    [Google Scholar]
  6. Galiana-Arnoux, D., Dostert, C., Schneemann, A., Hoffmann, J. A. & Imler, J. L. ( 2006; ). Essential function in vivo for Dicer-2 in host defense against RNA viruses in drosophila. Nat Immunol 7, 590–597.[CrossRef]
    [Google Scholar]
  7. Goto, A., Blandin, S., Royet, J., Reichhart, J. M. & Levashina, E. A. ( 2003; ). Silencing of Toll pathway components by direct injection of double-stranded RNA into Drosophila adult flies. Nucleic Acids Res 31, 6619–6623.[CrossRef]
    [Google Scholar]
  8. Johnson, K. N. & Christian, P. D. ( 1998; ). The novel genome organization of the insect picorna-like virus Drosophila C virus suggests this virus belongs to a previously undescribed virus family. J Gen Virol 79, 191–203.
    [Google Scholar]
  9. Johnson, K. N. & Christian, P. D. ( 1999; ). Molecular characterization of Drosophila C virus isolates. J Invertebr Pathol 73, 248–254.[CrossRef]
    [Google Scholar]
  10. Johnson, K. N., Zeddam, J. & Ball, L. A. ( 2000; ). Characterization and construction of functional cDNA clones of Pariacoto virus, the first Alphanodavirus isolated outside Australasia. J Virol 74, 5123–5132.[CrossRef]
    [Google Scholar]
  11. Jousset, F. X., Plus, N., Croizier, G. & Thomas, M. ( 1972; ). Existence chez Drosophila de deux groupes de picornavirus de propriétés sérologiques et biologiques différentes. C R Acad Sci Hebd Seances Acad Sci D 275, 3043–3046 (in French).
    [Google Scholar]
  12. L'Héritier, P. ( 1952; ). A convenient device for injecting large numbers of flies. Drosoph Inform Serv 26, 131
    [Google Scholar]
  13. Pfaffl, M. W., Horgan, G. W. & Dempfle, L. ( 2002; ). Relative expression software tool (rest) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res 30, e36 [CrossRef]
    [Google Scholar]
  14. Plus, N., Croizier, G., Jousset, F. X. & David, J. ( 1975; ). Picornaviruses of laboratory and wild Drosophila melanogaster: geographical distribution and serotypic composition. Ann Microbiol (Paris) 126A, 107–117.
    [Google Scholar]
  15. Reavy, B. & Moore, N. F. ( 1983; ). Cell-free translation of Drosophila C virus RNA: identification of a virus protease activity involved in capsid protein synthesis and further studies on in vitro processing of cricket paralysis virus specified proteins. Arch Virol 76, 101–115.[CrossRef]
    [Google Scholar]
  16. Roxstrom-Lindquist, K., Terenius, O. & Faye, I. ( 2004; ). Parasite-specific immune response in adult Drosophila melanogaster: a genomic study. EMBO Rep 5, 207–212.[CrossRef]
    [Google Scholar]
  17. Sabatier, L., Jouanguy, E., Dostert, C., Zachary, D., Dimarcq, J. L., Bulet, P. & Imler, J. L. ( 2003; ). Pherokine-2 and -3. Eur J Biochem 270, 3398–3407.[CrossRef]
    [Google Scholar]
  18. Scotti, P. D. ( 1977; ). End-point dilution and plaque assay methods for titration of cricket paralysis virus in cultured Drosophila cells. J Gen Virol 35, 393–396.[CrossRef]
    [Google Scholar]
  19. Tate, J., Liljas, L., Scotti, P., Christian, P., Lin, T. & Johnson, J. E. ( 1999; ). The crystal structure of cricket paralysis virus: the first view of a new virus family. Nat Struct Biol 6, 765–774.[CrossRef]
    [Google Scholar]
  20. Thomas-Orillard, M., Jeune, B. & Cusset, G. ( 1995; ). Drosophila-host genetic control of susceptibility to Drosophila C virus. Genetics 140, 1289–1295.
    [Google Scholar]
  21. van Rij, R. P., Saleh, M. C., Berry, B., Foo, C., Houk, A., Antoniewski, C. & Andino, R. ( 2006; ). The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster. Genes Dev 20, 2985–2995.[CrossRef]
    [Google Scholar]
  22. Wang, X. H., Aliyari, R., Li, W. X., Li, H. W., Kim, K., Carthew, R., Atkinson, P. & Ding, S. W. ( 2006; ). RNA interference directs innate immunity against viruses in adult Drosophila. Science 312, 452–454.[CrossRef]
    [Google Scholar]
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vol. , part 6, pp. 1497–1501

Primer Sequences for RT-qPCR and dsRNA synthesis [PDF](87 KB)



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