Restricted Semliki Forest virus replication in perforin and Fas-ligand double-deficient mice Free

Abstract

Previously, we have shown that mice defective in granule exocytosis and/or Fas.L/Fas-mediated cytolytic pathways are significantly more resistant to alphavirus, Semliki Forest virus (SFV), infection compared with wild-type mice. Here, we evaluated SFV replication in different tissues of mice defective in both cytolytic pathways (perfx) relative to that in wild-type counterparts and found that viral replication in perfx mice is remarkably restricted. Although the mechanism responsible for this observation is yet to be established, the lower virus titres found in these mice indicate that the role of cytolytic effector molecules in antiviral immunity needs to be re-evaluated.

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2008-08-01
2024-03-29
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References

  1. Alsharifi M., Lobigs M., Simon M. M., Kersten A., Muller K., Koskinen A., Lee E., Müllbacher A. 2006; NK cell-mediated immunopathology during an acute viral infection of the CNS. Eur J Immunol 36:887–896 [CrossRef]
    [Google Scholar]
  2. Atkins G. J., Sheahan B. J., Liljestrom P. 1999; The molecular pathogenesis of Semliki Forest virus: a model virus made useful?. J Gen Virol 80:2287–2297
    [Google Scholar]
  3. Bancroft G. J., Sheehan K. C., Schreiber R. D., Unanue E. R. 1989; Tumor necrosis factor is involved in the T cell-independent pathway of macrophage activation in scid mice. J Immunol 143:127–130
    [Google Scholar]
  4. Fazakerley J. K. 2002; Pathogenesis of Semliki Forest virus encephalitis. J Neurovirol 8:Suppl 266–74 [CrossRef]
    [Google Scholar]
  5. Fazakerley J. K., Pathak S., Scallan M., Amor S., Dyson H. 1993; Replication of the A7(74) strain of Semliki Forest virus is restricted in neurons. Virology 195:627–637 [CrossRef]
    [Google Scholar]
  6. Licon Luna R. M., Lee E., Müllbacher A., Blanden R. V., Langman R., Lobigs M. 2002; Lack of both Fas ligand and perforin protects from flavivirus-mediated encephalitis in mice. J Virol 76:3202–3211 [CrossRef]
    [Google Scholar]
  7. Mokhtarian F., Wesselingh S. L., Choi S., Maeda A., Griffin D. E., Sobel R. A., Grob D. 1996; Production and role of cytokines in the CNS of mice with acute viral encephalomyelitis. J Neuroimmunol 66:11–22 [CrossRef]
    [Google Scholar]
  8. Mosser D. M. 2003; The many faces of macrophage activation. J Leukoc Biol 73:209–212 [CrossRef]
    [Google Scholar]
  9. Müllbacher A., Blanden R. V. 1978; Murine cytotoxic T-cell response to alphavirus is associated mainly with H-2Dk . Immunogenetics 7:551–561 [CrossRef]
    [Google Scholar]
  10. Pathak S., Webb H. E. 1974; Possible mechanisms for the transport of Semliki forest virus into and within mouse brain. An electron-microscopic study. J Neurol Sci 23:175–184 [CrossRef]
    [Google Scholar]
  11. Soilu-Hanninen M., Eralinna J. P., Hukkanen V., Roytta M., Salmi A. A., Salonen R. 1994; Semliki Forest virus infects mouse brain endothelial cells and causes blood-brain barrier damage. J Virol 68:6291–6298
    [Google Scholar]
  12. Spielman J., Lee R. K., Podack E. R. 1998; Perforin/Fas-ligand double deficiency is associated with macrophage expansion and severe pancreatitis. J Immunol 161:7063–7070
    [Google Scholar]
  13. Wong G. H., Goeddel D. V. 1986; Tumour necrosis factors α and β inhibit virus replication and synergize with interferons. Nature 323:819–822 [CrossRef]
    [Google Scholar]
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