We previously reported the detection of bat coronaviruses (bat CoVs 1A, 1B, HKU7, HKU8 and bat-severe acute respiratory syndrome coronavirus) in Miniopterus spp. that cohabit a cave in Hong Kong. Here, we report the full genomic sequences of bat CoVs 1A, 1B and HKU8. Bat CoVs 1A and 1B, which are commonly found in the Miniopterus, are phylogenetically closely related. Using species-specific RT-PCR assays, bat CoVs 1A and 1B were confirmed to have distinct host specificities to Miniopterusmagnater and Miniopteruspusillus, respectively. Interestingly, co-infections of bat CoVs 1B and HKU8 in M. pusillus are detected in seven of 38 virus-positive specimens collected from 2004 to 2006. These findings highlight that co-infections of some coronaviruses might be common events in nature. The biological basis for the host restriction of bat coronaviruses, however, is yet to be determined.
BoschB. J.,
van der ZeeR.,
de HaanC. A.,
RottierP. J.2003; The coronavirus spike protein is a class I virus fusion protein: structural and functional characterization of the fusion core complex. J Virol 77:8801–8811[CrossRef]
ChenL. L.,
OuH. Y.,
ZhangR.,
ZhangC. T.2003; ZCURVE_CoV: a new system to recognize protein coding genes in coronavirus genomes, and its applications in analyzing SARS-CoV genomes. Biochem Biophys Res Commun 307:382–388[CrossRef]
DominguezS. R.,
O'SheaT. J.,
OkoL. M.,
HolmesK. V.2007; Detection of group 1 coronaviruses in bats in North America. Emerg Infect Dis 13:1295–1300[CrossRef]
DongB. Q.,
LiuW.,
FanX. H.,
VijaykrishnaD.,
TangX. C.,
GaoF.,
LiL. F.,
LiG. J.,
ZhangJ. X.other authors2007; Detection of a novel and highly divergent coronavirus from Asian leopard cats and Chinese ferret badgers in Southern China. J Virol 81:6920–6926[CrossRef]
EscorsD.,
CamafeitaE.,
OrtegoJ.,
LaudeH.,
EnjuanesL.2001; Organization of two transmissible gastroenteritis coronavirus membrane protein topologies within the virion and core. J Virol 75:12228–12240[CrossRef]
HaijemaB. J.,
VoldersH.,
RottierP. J.2004; Live, attenuated coronavirus vaccines through the directed deletion of group-specific genes provide protection against feline infectious peritonitis. J Virol 78:3863–3871[CrossRef]
HiscoxJ. A.,
MawdittK. L.,
CavanaghD.,
BrittonP.1995; Investigation of the control of coronavirus subgenomic mRNA transcription by using T7-generated negative-sense RNA transcripts. J Virol 69:6219–6227
HofmannM. A.,
ChangR. Y.,
KuS.,
BrianD. A.1993; Leader-mRNA junction sequences are unique for each subgenomic mRNA species in the bovine coronavirus and remain so throughout persistent infection. Virology 196:163–171[CrossRef]
LauS. K.,
WooP. C.,
LiK. S.,
HuangY.,
TsoiH. W.,
WongB. H.,
WongS. S.,
LeungS. Y.,
ChanK. H.,
YuenK. Y.2005; Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats. Proc Natl Acad Sci U S A 102:14040–14045[CrossRef]
LauS. K.,
WooP. C.,
LiK. S.,
HuangY.,
WangM.,
LamC. S.,
XuH.,
GuoR.,
ChanK. H.other authors2007; Complete genome sequence of bat coronavirus HKU2 from Chinese horseshoe bats revealed a much smaller spike gene with a different evolutionary lineage from the rest of the genome. Virology 367:428–439[CrossRef]
LiuP.,
LiL.,
MillershipJ. J.,
KangH.,
LeibowitzJ. L.,
GiedrocD. P.2007; A U-turn motif-containing stem-loop in the coronavirus 5′ untranslated region plays a functional role in replication. RNA 13:763–780[CrossRef]
PeirisJ. S.,
LaiS. T.,
PoonL. L.,
GuanY.,
YamL. Y.,
LimW.,
NichollsJ.,
YeeW. K.,
YanW. W.other authors2003; Coronavirus as a possible cause of severe acute respiratory syndrome. Lancet 361:1319–1325[CrossRef]
PoonL. L.,
ChuD. K.,
ChanK. H.,
WongO. K.,
EllisT. M.,
LeungY. H.,
LauS. K.,
WooP. C.,
SuenK. Y.other authors2005; Identification of a novel coronavirus in bats. J Virol 79:2001–2009[CrossRef]
PyrcK.,
JebbinkM. F.,
BerkhoutB.,
van der HoekL.2004; Genome structure and transcriptional regulation of human coronavirus NL63. Virol J 1: 7[CrossRef]
TungF. Y.,
AbrahamS.,
SethnaM.,
HungS. L.,
SethnaP.,
HogueB. G.,
BrianD. A.1992; The 9-kDa hydrophobic protein encoded at the 3′ end of the porcine transmissible gastroenteritis coronavirus genome is membrane-associated. Virology 186:676–683[CrossRef]