1887

Abstract

Of all human endogenous retroviruses known today, HERV-K is the only one that has been shown to produce viral particles. While the first of the approximately 30 HERV-K sequences integrated into the human genome more than 40 million years ago, evidence is accumulating that HERV-K was active more recently, provirus HERV-K113 being the youngest sequence found. However, it is unclear which HERV-K sequences code for the viral particles that are produced by human germ-cell tumours or melanomas. Here, we show that the provirus HERV-K113, cloned into a baculovirus expression vector, is capable of producing intact particles of retroviral morphology, exhibiting the typical structure of those particles that were characterized in cell lines derived from human germ-cell tumours. Thus, the HERV-K113 sequence is a candidate for particle production and for an active human endogenous retrovirus of today.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.83534-0
2008-02-01
2020-09-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/89/2/567.html?itemId=/content/journal/jgv/10.1099/vir.0.83534-0&mimeType=html&fmt=ahah

References

  1. Bannert N., Kurth R. 2004; Retroelements and the human genome: new perspectives on an old relation. Proc Natl Acad Sci U S A 101:Suppl. 214572–14579 [CrossRef]
    [Google Scholar]
  2. Belshaw R., Pereira V., Katzourakis A., Talbot G., Paces J., Burt A., Tristem M. 2004; Long-term reinfection of the human genome by endogenous retroviruses. Proc Natl Acad Sci U S A 101:4894–4899 [CrossRef]
    [Google Scholar]
  3. Bieda K., Hoffmann A., Boller K. 2001; Phenotypic heterogeneity of human endogenous retrovirus particles produced by teratocarcinoma cell lines. J Gen Virol 82:591–596
    [Google Scholar]
  4. Boller K., Frank H., Löwer J., Löwer R., Kurth R. 1983; Structural organization of unique retrovirus-like particles budding from human teratocarcinoma cell lines. J Gen Virol 64:2549–2559 [CrossRef]
    [Google Scholar]
  5. Boller K., König H., Sauter M., Mueller-Lantzsch N., Löwer R., Löwer J., Kurth R. 1993; Evidence that HERV-K is the endogenous retrovirus sequence that codes for the human teratocarcinoma-derived retrovirus HTDV. Virology 196:349–353 [CrossRef]
    [Google Scholar]
  6. Boller K., Janssen O., Schuldes H., Tönjes R. R., Kurth R. 1997; Characterization of the antibody response specific for the human endogenous retrovirus HTDV/HERV-K. J Virol 71:4581–4588
    [Google Scholar]
  7. Büscher K., Hahn S., Hofmann M., Trefzer U., Özel M., Sterry W., Löwer J., Löwer R., Kurth R., Denner J. 2006; Expression of the human endogenous retrovirus-K transmembrane envelope, Rec and Np9 proteins in melanomas and melanoma cell lines. Melanoma Res 16:223–234 [CrossRef]
    [Google Scholar]
  8. Dewannieux M., Blaise S., Heidmann T. 2005; Identification of a functional envelope protein from the HERV-K family of human endogenous retroviruses. J Virol 79:15573–15577 [CrossRef]
    [Google Scholar]
  9. Dewannieux M., Harper F., Richaud A., Letzelter C., Ribet D., Pierron G., Heidmann T. 2006; Identification of an infectious progenitor for the multiple-copy HERV-K human endogenous retroelements. Genome Res 16:1548–1556 [CrossRef]
    [Google Scholar]
  10. Jeang K. T., Holmgren-Konig M., Khoury G. 1987; A baculovirus vector can express intron-containing genes. J Virol 61:1761–1764
    [Google Scholar]
  11. Kleiman A., Senyuta N., Tryakin A., Sauter M., Karseladze A., Tjulandin S., Gurtsevitch V., Mueller-Lantzsch N. 2004; HERV-K(HML-2) GAG/ENV antibodies as indicator for therapy effect in patients with germ cell tumors. Int J Cancer 110:459–461 [CrossRef]
    [Google Scholar]
  12. Lander E. S., Linton L. M., Birren B., Nusbaum C., Zody M. C., Baldwin J., Devon K., Dewar K., Doyle M. other authors 2001; Initial sequencing and analysis of the human. Nature 409:860–921 [CrossRef]
    [Google Scholar]
  13. Lee Y. N., Bieniasz P. D. 2007; Reconstitution of an infectious human endogenous retrovirus. PLoS Pathog 3:e10 [CrossRef]
    [Google Scholar]
  14. Löwer R., Löwer J., Frank H., Harzmann R., Kurth R. 1984; Human teratocarcinomas cultured in vitro produce unique retrovirus-like viruses. J Gen Virol 65:887–898 [CrossRef]
    [Google Scholar]
  15. Löwer R., Boller K., Hasenmaier B., Korbmacher C., Müller-Lantzsch N., Löwer J., Kurth R. 1993; Identification of human endogenous retroviruses with complex mRNA expression and particle formation. Proc Natl Acad Sci U S A 90:4480–4484 [CrossRef]
    [Google Scholar]
  16. Lugert R., König H., Kurth R., Tönjes R. R. 1996; Specific suppression of false-positive signals in the product-enhanced reverse transcriptase assay. Biotechniques 20:210–217
    [Google Scholar]
  17. Medstrand P., Lindeskog M., Blomberg J. 1992; Expression of human endogenous retroviral sequences in peripheral blood mononuclear cells of healthy individuals. J Gen Virol 73:2463–2466 [CrossRef]
    [Google Scholar]
  18. Moyes D. L., Martin A., Sawcer S., Temperton N., Worthington J., Griffiths D. J., Venables P. J. 2005; The distribution of the endogenous retroviruses HERV-K113 and HERV-K115 in health and disease. Genomics 86:337–341 [CrossRef]
    [Google Scholar]
  19. Mueller-Lantzsch N., Sauter M., Weiskircher A., Kramer K., Best B., Buck M., Grasser F. 1993; Human endogenous retroviral element K10 (HERV-K10) encodes a full-length gag homologous 73-kDa protein and a functional protease. AIDS Res Hum Retroviruses 9:343–350 [CrossRef]
    [Google Scholar]
  20. Muster T., Waltenberger A., Grassauer A., Hirschl S., Caucig P., Romirer I., Fodinger D., Seppele H., Schanab O. other authors 2003; An endogenous retrovirus derived from human melanoma cells. Cancer Res 63:8735–8741
    [Google Scholar]
  21. Sauter M., Schommer S., Kremmer E., Remberger K., Dolken G., Lemm I., Buck M., Best B., Neumann-Haefelin D., Mueller-Lantzsch N. 1995; Human endogenous retrovirus K10: expression of Gag protein and detection of antibodies in patients with seminomas. J Virol 69:414–421
    [Google Scholar]
  22. Schommer S., Sauter M., Kräusslich H. G., Best B., Mueller-Lantzsch N. 1996; Characterization of the human endogenous retrovirus K proteinase. J Gen Virol 77:375–379 [CrossRef]
    [Google Scholar]
  23. Silver J., Maudru T., Fujita K., Repaske R. 1993; An RT-PCR assay for the enzyme activity of reverse transcriptase capable of detecting single virions. Nucleic Acids Res 21:3593–3594 [CrossRef]
    [Google Scholar]
  24. Tokuyasu K. T., Dutton A. H., Geiger B., Singer S. J. 1981; Ultrastructure of chicken cardiac muscle as studied by double immunolabeling in electron microscopy. Proc Natl Acad Sci U S A 78:7619–7623 [CrossRef]
    [Google Scholar]
  25. Tönjes R. R., Boller K., Limbach C., Lugert R., Kurth R. 1997; Characterization of human endogenous retrovirus type K virus-like particles generated from recombinant baculoviruses. Virology 233:280–291 [CrossRef]
    [Google Scholar]
  26. Turner G., Barbulescu M., Su M., Jensen-Seaman M. I., Kidd K. K., Lenz J. 2001; Insertional polymorphisms of full-length endogenous retroviruses in humans. Curr Biol 11:1531–1535 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.83534-0
Loading
/content/journal/jgv/10.1099/vir.0.83534-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error