1887

Abstract

APOBEC3G (A3G), a member of cytidine deaminase family, has potent anti-human immunodeficiency virus type 1 (HIV-1) activity. It has been demonstrated that alpha interferon (IFN-) can significantly enhance the expression of A3G in human primary resting CD4 T-cells, macrophages and primary hepatocytes, subsequently decreasing their viral susceptibility. Plasmacytoid dendritic cells (pDCs) are key effectors in innate host immunity, mediating adaptive immune responses and stimulating IFN- production in reaction to various stimuli. In this report, we demonstrate that IFN-, either exogenously added to- or endogenously secreted by pDCs, can enhance the expression of A3G and its family members such as A3A, A3C and A3F. We have also shown that IFN- can inhibit HIV-1 expression in pDCs. This inhibitory effect could be countered by addition of an A3G-specific short interfering RNA, indicating that IFN--induced A3G plays a key role in mediating pDCs response to HIV-1. Given the central role played by pDCs in orchestrating the IFN-/A3G intercellular network and intracellular signal pathway, our data indicate that pDCs themselves are also protected by an IFN-/A3G-mediated innate immunity barrier from HIV-1 infection.

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2008-03-01
2020-01-17
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