1887

Abstract

In this report, the open reading frame 21 () of nucleopolyhedrovirus (BmNPV), one of the unique genes of group I NPVs, was characterized. is predicted to encode a protein of 55.8 kDa and was found to contain imperfectly conserved leucine-rich repeats. 3′ Rapid amplification of cDNA ends (3′RACE) showed that the transcript of was first detected from 6 h post-infection and that it also encompassed the complete . 5′RACE revealed three transcription initiation sites, one of which mapped to the baculovirus early transcription motifs CGTGC and CAGT. Transient-expression and superinfection assays indicated that BM21 localized in the nucleus of infected BmN cells. To study the function of BM21, a -null virus was constructed using bacmid technology. Viral one-step growth curve analyses showed that the -null virus had similar budded virus production kinetics to those of the parental virus. Bioassay analyses showed that the median lethal concentration (LC) of the -null virus was similar to that of the control virus; however, the median survival time (ST) of the knockout virus was significantly longer than the control virus. These results indicate that BM21 is not essential for virus replication , but that deletion of the gene delays the killing of the infected larvae.

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2008-04-01
2020-10-26
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