1887

Abstract

In this report, the open reading frame 21 () of nucleopolyhedrovirus (BmNPV), one of the unique genes of group I NPVs, was characterized. is predicted to encode a protein of 55.8 kDa and was found to contain imperfectly conserved leucine-rich repeats. 3′ Rapid amplification of cDNA ends (3′RACE) showed that the transcript of was first detected from 6 h post-infection and that it also encompassed the complete . 5′RACE revealed three transcription initiation sites, one of which mapped to the baculovirus early transcription motifs CGTGC and CAGT. Transient-expression and superinfection assays indicated that BM21 localized in the nucleus of infected BmN cells. To study the function of BM21, a -null virus was constructed using bacmid technology. Viral one-step growth curve analyses showed that the -null virus had similar budded virus production kinetics to those of the parental virus. Bioassay analyses showed that the median lethal concentration (LC) of the -null virus was similar to that of the control virus; however, the median survival time (ST) of the knockout virus was significantly longer than the control virus. These results indicate that BM21 is not essential for virus replication , but that deletion of the gene delays the killing of the infected larvae.

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2008-04-01
2024-12-06
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References

  1. Afonso C. L., Tulman E. R., Lu Z., Oma E., Kutish G. F., Rock D. L. 1999; The genome of Melanoplus sanguinipes entomopoxvirus. J Virol 73:533–552
    [Google Scholar]
  2. Bawden A. L., Glassberg K. J., Diggans J., Shaw R., Farmerie W., Moyer R. W. 2000; Complete genomic sequence of the Amsacta moorei entomopoxvirus: analysis and comparison with other poxviruses. Virology 274:120–139 [CrossRef]
    [Google Scholar]
  3. Beniya H., Braunagel S. C., Summers M. D. 1998; Autographa californica nuclear polyhedrosis virus: subcellular localization and protein trafficking of BV/ODV-E26 to intranuclear membranes and viral envelopes. Virology 240:64–75 [CrossRef]
    [Google Scholar]
  4. Bilder D., Perrimon N. 2000; Localization of apical epithelial determinants by the basolateral PDZ protein Scribble. Nature 403:676–680 [CrossRef]
    [Google Scholar]
  5. Blissard G. W., Wenz J. R. 1992; Baculovirus gp64 envelope glycoprotein is sufficient to mediate pH-dependent membrane fusion. J Virol 66:6829–6835
    [Google Scholar]
  6. Bulach D. M., Kumar C. A., Zaia A., Liang B., Tribe D. E. 1999; Group II nucleopolyhedrovirus subgroups revealed by phylogenetic analysis of polyhedrin and DNA polymerase gene sequences. J Invertebr Pathol 73:59–73 [CrossRef]
    [Google Scholar]
  7. Burks J. K., Summers M. D., Braunagel S. C. 2007; BV/ODV-E26: a palmitoylated, multifunctional structural protein of Autographa californica nucleopolyhedrovirus. Virology 361:194–203 [CrossRef]
    [Google Scholar]
  8. Carson D. D., Guarino L. A., Summers M. D. 1988; Functional mapping of an AcNPV immediately early gene which augments expression of the IE-1 trans-activated 39K gene. Virology 162:444–451 [CrossRef]
    [Google Scholar]
  9. Chen X., Sun X., Hu Z., Li M., O'Reilly D. R., Zuidema D., Vlak J. M. 2000; Genetic engineering of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus as an improved pesticide. J Invertebr Pathol 76:140–146 [CrossRef]
    [Google Scholar]
  10. Choudary P. V., Kamita S. G., Maeda S. 1995 Baculovirus Expression Protocols Totowa, NJ: Humana Press;
    [Google Scholar]
  11. Dall D., Luque T., O'Reilly D. 2001; Insect–virus relationships: sifting by informatics. Bioessays 23:184–193 [CrossRef]
    [Google Scholar]
  12. Detvisitsakun C., Cain E. L., Passarelli A. L. 2007; The Autographa californica M nucleopolyhedrovirus fibroblast growth factor accelerates host mortality. Virology 365:70–78 [CrossRef]
    [Google Scholar]
  13. Evdokimov A. G., Anderson D. E., Routzahn K. M., Waugh D. S. 2001; Unusual molecular architecture of the Yersinia pestis cytotoxin YopM: a leucine-rich repeat protein with the shortest repeating unit. J Mol Biol 312:807–821 [CrossRef]
    [Google Scholar]
  14. Flipsen J. T., Mans R. M., Kleefsman A. W., Knebel-Morsdorf D., Vlak J. M. 1995; Deletion of the baculovirus ecdysteroid UDP-glucosyltransferase gene induces early degeneration of Malpighian tubules in infected insects. J Virol 69:4529–4532
    [Google Scholar]
  15. Gomi S., Majima K., Maeda S. 1999; Sequence analysis of the genome of Bombyx mori nucleopolyhedrovirus. J Gen Virol 80:1323–1337
    [Google Scholar]
  16. Hake S. B., Masternak K., Kammerbauer C., Janzen C., Reith W., Steimle V. 2000; CIITA leucine-rich repeats control nuclear localization, in vivo recruitment to the major histocompatibility complex (MHC) class II enhanceosome, and MHC class II gene transactivation. Mol Cell Biol 20:7716–7725 [CrossRef]
    [Google Scholar]
  17. Hefferon K. L., Oomens A. G. P., Monsma S. A., Finnerty C. M., Blissard G. W. 1999; Host cell receptor binding by baculovirus GP64 and kinetics of virion entry. Virology 258:455–468 [CrossRef]
    [Google Scholar]
  18. Herniou E. A., Luque T., Chen X., Vlak J. M., Winstanley D., Cory J. S., O'Reilly D. R. 2001; Use of whole genome sequence data to infer baculovirus phylogeny. J Virol 75:8117–8126 [CrossRef]
    [Google Scholar]
  19. Hou S., Chen X., Wang H., Tao M., Hu Z. 2002; Efficient method to generate homologous recombinant baculovirus genomes in E. coli . Biotechniques32783–784 786:788
    [Google Scholar]
  20. Huang J., Hao B., Sun X., Deng F., Wang H., Hu Z. 2007; Construction of the Bac-to-Bac system of Bombyx mori nucleopolyhedrovirus. Virol Sin 22:218–225 [CrossRef]
    [Google Scholar]
  21. Ikeda M., Shikata M., Shirata N., Chaeychomsri S., Kobayashi M. 2006; Gene organization and complete sequence of the Hyphantria cunea nucleopolyhedrovirus genome. J Gen Virol 87:2549–2562 [CrossRef]
    [Google Scholar]
  22. Imai N., Kurihara M., Matsumoto S., Kang W. K. 2004; Bombyx mori nucleopolyhedrovirus orf8 encodes a nucleic acid binding protein that colocalizes with IE1 during infection. Arch Virol 149:1581–1594
    [Google Scholar]
  23. Inohara N., Koseki T., del Peso L., Hu Y., Yee C., Chen S., Carrio R., Merino J., Liu D. other authors 1999; Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor- κ B. J Biol Chem 274:14560–14567 [CrossRef]
    [Google Scholar]
  24. Jans D. A., Hubner S. 1996; Regulation of protein transport to the nucleus: central role of phosphorylation. Physiol Rev 76:651–685
    [Google Scholar]
  25. Jones D. A., Jones J. D. G. 1997; The role of leucine-rich repeat proteins in plant defences. Adv Bot Res 24:89–167
    [Google Scholar]
  26. Kalbfleisch J. D., Ross L. 1980 The Statistical Analysis of Failure Time Data New York: Wiley;
    [Google Scholar]
  27. Kamita S. G., Nagasaka K., Chua J. W., Shimada T., Mita K., Kobayashi M., Maeda S., Hammock B. D. 2005; A baculovirus-encoded protein tyrosine phosphatase gene induces enhanced locomotory activity in a lepidopteran host. Proc Natl Acad Sci U S A 102:2584–2589 [CrossRef]
    [Google Scholar]
  28. Katsuma S., Shimada T., Kobayashi M. 2004; Characterization of the baculovirus Bombyx mori nucleopolyhedrovirus gene homologous to the mammalian FGF gene family. Virus Genes 29:211–217 [CrossRef]
    [Google Scholar]
  29. Katsuma S., Horie S., Daimon T., Iwanaga M., Shimada T. 2006; In vivo and in vitro analyses of a Bombyx mori nucleopolyhedrovirus mutant lacking functional vfgf . Virology 355:62–70 [CrossRef]
    [Google Scholar]
  30. Kobe B., Kajava A. V. 2001; The leucine-rich repeat as a protein recognition motif. Curr Opin Struct Biol 11:725–732 [CrossRef]
    [Google Scholar]
  31. Kool M., Ahrens C. H., Goldbach R. W., Rohrmann G. F., Vlak J. M. 1994; Identification of genes involved in DNA replication of the Autographa californica baculovirus. Proc Natl Acad Sci U S A 91:11212–11216 [CrossRef]
    [Google Scholar]
  32. Lapointe R., Back D. W., Ding Q., Carstens E. B. 2000; Identification and molecular characterization of the Choristoneura fumiferana multicapsid nucleopolyhedrovirus genomic region encoding the regulatory genespkip , p47 ,lef-12 , and gta . Virology 271: 109–121 [CrossRef]
    [Google Scholar]
  33. Li Y., Miller L. K. 1995; Expression and localization of a baculovirus protein phosphatase. J Gen Virol 76:2941–2948 [CrossRef]
    [Google Scholar]
  34. Linhoff M. W., Harton J. A., Cressman D. E., Martin B. K., Ting J. P. 2001; Two distinct domains within CIITA mediate self-association: involvement of the GTP-binding and leucine-rich repeat domains. Mol Cell Biol 21:3001–3011 [CrossRef]
    [Google Scholar]
  35. Lu A., Miller L. K. 1995; The roles of eighteen baculovirus late expression factor genes in transcription and DNA replication. J Virol 69:975–982
    [Google Scholar]
  36. Lung O. Y., Cruz-Alvarez M., Blissard G. W. 2003; Ac23, an envelope fusion protein homolog in the baculovirus Autographa californica multicapsid nucleopolyhedrovirus, is a viral pathogenicity factor. J Virol 77:328–339 [CrossRef]
    [Google Scholar]
  37. Mainz D., Quadt I., Knebel-Morsdorf D. 2002; Nuclear IE2 structures are related to viral DNA replication sites during baculovirus infection. J Virol 76:5198–5207 [CrossRef]
    [Google Scholar]
  38. Marino M., Braun L., Cossart P., Ghosh P. 1999; Structure of the InlB leucine-rich repeats, a domain that triggers host cell invasion by the bacterial pathogen L. monocytogenes . Mol Cell 4:1063–1072 [CrossRef]
    [Google Scholar]
  39. Mutai H., Toyoshima Y., Sun W., Hattori N., Tanaka S., Shiota K. 2000; PAL31, a novel nuclear protein, expressed in the developing brain. Biochem Biophys Res Commun 274:427–433 [CrossRef]
    [Google Scholar]
  40. O'Reilly D. R., Miller L. K. 1989; A baculovirus blocks insect molting by producing ecdysteroid UDP-glucosyl transferase. Science 245:1110 [CrossRef]
    [Google Scholar]
  41. O'Reilly D. R., Miller L. K. 1991; Improvement of a baculovirus pesticide by deletion of the EGT gene. Biotechnology 9:1086–1089 [CrossRef]
    [Google Scholar]
  42. O'Reilly D. R., Luckow V. A., Miller L. K. 1992 Baculovirus Expression Vectors: a Laboratory Manual New York: W. H. Freeman;
    [Google Scholar]
  43. Oomens A. G. P., Blissard G. W. 1999; Requirement for GP64 to drive efficient budding of Autographa californica multicapsid nucleopolyhedrovirus. Virology 254:297–314 [CrossRef]
    [Google Scholar]
  44. Prikhod'ko E. A., Miller L. K. 1998; Role of baculovirus IE2 and its RING finger in cell cycle arrest. J Virol 72:684–692
    [Google Scholar]
  45. Reisner B. S., Straley S. C. 1992; Yersinia pestis YopM: thrombin binding and overexpression. Infect Immun 60:5242–5252
    [Google Scholar]
  46. Saville G. P., Patmanidi A. L., Possee R. D., King L. A. 2004; Deletion of the Autographa californica nucleopolyhedrovirus chitinase KDEL motif and in vitro and in vivo analysis of the modified virus. J Gen Virol 85:821–831 [CrossRef]
    [Google Scholar]
  47. Shippam-Brett C. E., Willis L. G., Theilmann D. A. 2001; Analysis of sequences involved in IE2 transactivation of a baculovirus immediate-early gene promoter and identification of a new regulatory motif. Virus Res 75:13–28 [CrossRef]
    [Google Scholar]
  48. Snedecor G. W. 1989 Statistical Methods, 8th edn. Ames, Iowa: Iowa State University Press;
    [Google Scholar]
  49. Yoo S., Guarino L. A. 1994a; The Autographa californica nuclear polyhedrosis virus ie2 gene encodes a transcriptional regulator. Virology 202:746–753 [CrossRef]
    [Google Scholar]
  50. Yoo S., Guarino L. A. 1994b; Functional dissection of the ie2 gene product of the baculovirus Autographa californica nuclear polyhedrosis virus. Virology 202:164–172 [CrossRef]
    [Google Scholar]
  51. Zanotto P. M., Kessing B. D., Maruniak J. E. 1993; Phylogenetic interrelationships among baculoviruses: evolutionary rates and host associations. J Invertebr Pathol 62:147–164 [CrossRef]
    [Google Scholar]
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