1887

Abstract

F proteins of group II nucleopolyhedroviruses (NPVs) are envelope fusion proteins essential for virus entry and egress. An F-null single nucleocapsid NPV (HearNPV) bacmid, HaBacΔF, was constructed. This bacmid could not produce infectious budded virus (BV) when transfected into HzAM1 cells, showing that F protein is essential for cell-to-cell transmission of BVs. When HaBacΔF was pseudotyped with the homologous F protein (HaBacΔF-HaF, positive control) or with the heterologous F protein from multinucleocapsid NPV (SeMNPV) (HaBacΔF-SeF), infectious BVs were produced with similar kinetics. In the late phase of infection, the BV titre of HaBacΔF-SeF virus was about ten times lower than that of HaBacΔF-HaF virus. Both pseudotyped viruses were able to fuse HzAM1 cells in a similar fashion. The F proteins of both HearNPV and SeMNPV were completely cleaved into F and F in the BVs of vHaBacΔF-HaF and vHaBacΔF-SeF, respectively, but the cleavage of SeF in vHaBacΔF-SeF-infected HzAM1 cells was incomplete, explaining the lower BV titre of vHaBacΔF-SeF. Polyclonal antisera against HaF and SeF specifically neutralized the infection of vHaBacΔF-HaF and vHaBacΔF-SeF, respectively. HaF antiserum showed some cross-neutralization with vHaBacΔF-SeF. These results demonstrate that group II NPV F proteins can be functionally replaced with a homologue of other group II NPVs, suggesting that the interaction of F with other viral or host proteins is not absolutely species-specific.

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2008-03-01
2020-07-13
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References

  1. Ahrens C. H., Russell R., Funk C. J., Evans J. T., Harwood S. H., Rohrmann G. F. 1997; The sequence of the Orgyia pseudotsugata multinucleocapsid polyhedrosis virus genome. Virology 229:381–399 [CrossRef]
    [Google Scholar]
  2. Ayres M. D., Howard S. C., Kuzio J., Lopez-Ferber M., Possee R. D. 1994; The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology 202:586–605 [CrossRef]
    [Google Scholar]
  3. Blissard G. W., Wenz J. R. 1992; Baculovirus gp64 envelope glycoprotein is sufficient to mediate pH-dependent membrane fusion. J Virol 66:6829–6835
    [Google Scholar]
  4. Gomi S., Majima K., Maeda S. 1999; Sequence analysis of the genome of Bombyx mori nucleopolyhedrovirus. J Gen Virol 80:1323–1337
    [Google Scholar]
  5. Hallenberger S., Bosch V., Angliker H., Shaw E., Klenk H.-D., Garten W. 1992; Inhibition of furin-mediated cleavage activation of HIV-1 glycoprotein gp160. Nature 360:358–361 [CrossRef]
    [Google Scholar]
  6. Hefferon K. L., Oomens A. G. P., Monsma S. A., Finnerty C. M., Blissard G. W. 1999; Host cell receptor binding by baculovirus GP64 and kinetics of virion entry. Virology 258:455–468 [CrossRef]
    [Google Scholar]
  7. Hosaka M., Nagahama M., Kim W. S., Watanabe T., Hatsuzawa K., Ikemizu J., Murakami K., Nakayama K. 1991; Arg-X-Lys/Arg-Arg motif as a signal for precursor cleavage catalyzed by furin within the constitutive secretory pathway. J Biol Chem 266:12127–12130
    [Google Scholar]
  8. Hou S., Chen X., Wang H., Tao M., Hu Z. 2002; Efficient method to generate homologous recombinant baculovirus genomes in E. coli . Biotechniques32783–789
    [Google Scholar]
  9. IJkel W. F. J., Westenberg M., Goldbach R. W., Blissard G. W., Vlak J. M., Zuidema D. 2000; A novel baculovirus envelope fusion protein with a proprotein convertase cleavage site. Virology 275:30–41 [CrossRef]
    [Google Scholar]
  10. Kingsley D. H., Behbahani A., Rashtian A., Blissard G. W., Zimmerberg J. 1999; A discrete stage of baculovirus GP64-mediated membrane fusion. Mol Biol Cell 10:4191–4200 [CrossRef]
    [Google Scholar]
  11. Lazarowitz S. G., Comapans R. W., Choppin P. W. 1971; Influenza virus structural and nonstructural proteins in infected cells and their plasma membrane. Virology 46:830–843 [CrossRef]
    [Google Scholar]
  12. Liang C., Song J., Chen X. 2005; The GP64 protein of Autographa californica multiple nucleopolyhedrovirus rescues Helicoverpa armigera nucleopolyhedrovirus transduction in mammalian cells. J Gen Virol 86:1629–1635 [CrossRef]
    [Google Scholar]
  13. Long G., Westenberg M., Wang H., Vlak J. M., Hu Z. 2006a; Function, oligomerization and N -linked glycosylation of the Helicoverpa armigera single nucleopolyhedrovirus envelope fusion protein. J Gen Virol 87:839–846 [CrossRef]
    [Google Scholar]
  14. Long G., Pan X., Westenberg M., Vlak J. M. 2006b; Functional role of the cytoplasmic tail domain of the major envelope fusion protein of group II baculoviruses. J Virol 80:11226–11234 [CrossRef]
    [Google Scholar]
  15. Lung O., Westenberg M., Vlak J. M., Zuidema D., Blissard G. W. 2002; Pseudotyping Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV): F proteins from group II NPVs are functionally analogous to AcMNPV GP64. J Virol 76:5729–5736 [CrossRef]
    [Google Scholar]
  16. Lung O. Y., Cruz-Alvarez M., Blissard G. W. 2003; Ac23, an envelope fusion protein homolog in the baculovirus Autographa californica multicapsid nucleopolyhedrovirus, is a viral pathogenicity factor. J Virol 77:328–339 [CrossRef]
    [Google Scholar]
  17. Meyer H., Masuho Y., Mach M. 1990; The gp116 of the gp58/116 complex of human cytomegalovirus represents the amino-terminal part of the precursor molecule and contains a neutralizing epitope. J Gen Virol 71:2443–2450 [CrossRef]
    [Google Scholar]
  18. Monsma S. A., Oomens A. G. P., Blissard G. W. 1996; The GP64 envelope fusion protein is an essential baculovirus protein required for cell-to-cell transmission of infection. J Virol 70:4607–4616
    [Google Scholar]
  19. Morse M. A., Marriott A. C., Nuttall P. A. 1992; The glycoprotein of Thogoto virus (a tick-borne orthomyxo-like virus) is related to the baculovirus glycoprotein GP64. Virology 186:640–646 [CrossRef]
    [Google Scholar]
  20. Moulard M., Hallenberger S., Garten W., Klenk H.-D. 1999; Processing and routage of HIV glycoproteins by furin to the cell surface. Virus Res 60:55–65 [CrossRef]
    [Google Scholar]
  21. Oomens A. G. P., Blissard G. W. 1999; Requirement for GP64 to drive efficient budding of Autographa californica multicapsid nucleopolyhedrovirus. Virology 254:297–314 [CrossRef]
    [Google Scholar]
  22. Oomens A. G. P., Monsma S. A., Blissard G. W. 1995; The baculovirus GP64 envelope fusion protein: synthesis, oligomerization, and processing. Virology 209:592–603 [CrossRef]
    [Google Scholar]
  23. Pearson M. N., Groten C., Rohrmann G. F. 2000; Identification of the Lymantria dispar nucleopolyhedrovirus envelope fusion protein provides evidence for a phylogenetic division of the Baculoviridae . J Virol 74:6126–6131 [CrossRef]
    [Google Scholar]
  24. Scheid A., Choppin P. W. 1977; Two disulfide-linked polypeptide chains constitute the active F protein of paramyxoviruses. Virology 80:54–66 [CrossRef]
    [Google Scholar]
  25. Toyoda T., Gotoh B., Sakaguchi T., Kida H., Nagai Y. 1988; Identification of amino acids relevant to three antigenic determinants on the fusion protein of Newcastle disease virus that are involved in fusion inhibition and neutralization. J Virol 62:4427–4430
    [Google Scholar]
  26. Veronese F. D., DeVico A. L., Copeland T. D., Oroszlan S., Gallo R. C., Sarngadharan M. G. 1985; Characterization of gp41 as the transmembrane protein coded by the HTLV-III/LAV envelope gene. Science 229:1402–1405 [CrossRef]
    [Google Scholar]
  27. Vey M., Schäfer W., Berghöfer S., Klenk H. D., Garten W. 1994; Maturation of the trans-Golgi network protease furin: compartmentalization of propeptide removal, substrate cleavage, and COOH-terminal truncation. J Cell Biol 127:1829–1842 [CrossRef]
    [Google Scholar]
  28. Wang H., Deng F., Pijlman G. P., Chen X., Sun X., Vlak J. M., Hu Z. 2003; Cloning of biologically active genomes from a Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus isolate by using a bacterial artificial chromosome. Virus Res 97:57–63 [CrossRef]
    [Google Scholar]
  29. Westenberg M., Vlak J. M. 2008; GP64 of group I nucleopolyhedroviruses cannot readily rescue infectivity of group II f -null nucleopolyhedroviruses. J Gen Virol 89:424–431 [CrossRef]
    [Google Scholar]
  30. Westenberg M., Wang H., IJkel W. F. J., Goldbach R. W., Vlak J. M., Zuidema D. 2002; Furin is involved in baculovirus envelope fusion protein activation. J Virol 76:178–184 [CrossRef]
    [Google Scholar]
  31. Westenberg M., Veenman F., Roode E. C., Goldbach R. W., Vlak J. M., Zuidema D. 2004; Functional analysis of the putative fusion domain of the baculovirus envelope fusion protein. J Virol 78:6946–6954 [CrossRef]
    [Google Scholar]
  32. Westenberg M., Uijtdewilligen P., Vlak J. M. 2007; Baculovirus envelope fusion proteins F and GP64 exploit distinct receptors to gain entry into cultured insect cells. J Gen Virol 88:3302–3306 [CrossRef]
    [Google Scholar]
  33. Zolla-Pazner S., Gomy M. K., Nyambi P. N. 1999; The implications of antigenic diversity for vaccine development. Immunol Lett 66:159–164 [CrossRef]
    [Google Scholar]
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