1887

Abstract

Arenaviruses include several causative agents of haemorrhagic fever disease in humans. In addition, the prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) is a superb model for the study of virus–host interactions, including the basis of viral persistence and associated diseases. There is little understanding about the molecular mechanisms concerning the regulation and specific role of viral proteins in modulating arenavirus–host cell interactions either associated with an acute or persistent infection, and associated disease. Here, we report the genomic and biological characterization of LCMV strains ‘Docile’ (persistent) and ‘Aggressive’ (not persistent) recovered from cloned cDNA via reverse genetics. Our results confirmed that the cloned viruses accurately recreated the phenotypes associated with the corresponding natural Docile and Aggressive viral isolates. In addition, we provide evidence that the ability of the Docile strain to persist is determined by the nature of both S and L RNA segments. Thus, our findings provide the foundation for studies aimed at gaining a detailed understanding of viral determinants of LCMV persistence in its natural host, which may aid in the development of vaccines to prevent or treat the diseases caused by arenaviruses in humans.

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2008-06-01
2020-01-24
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vol. , part 6, pp. 1421 - 1433

Viral genomic sense cDNA sequence of the S RNA of Doc

Viral genomic sense cDNA sequence of the L RNA of Doc

Viral genomic sense cDNA sequence of the S RNA of Agg

Viral genomic sense cDNA sequence of the L RNA of Agg

Biological properties of rDoc, rAgg, rArm, rSDocLAgg or rSArmLAgg strains

The molecular structure of both S and L RNA segments of Doc contribute to shaping the persistent viral phenotype [Single PDF file](2525 KB)



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