1887

Abstract

The open reading frame of single nucleocapsid nucleopolyhedrovirus (HearNPV) encodes a putative protein of 51 kDa with homologues in a few group II NPVs and a granulovirus. was transcribed as polyadenylated transcripts in infected HzAM1 insect cells. The transcripts were initiated at two distinct locations, one upstream of (superoxide dismutase gene, ) and the second upstream of . By Western blot analysis, two forms of the HA107 protein were detected in infected cells, a major polypeptide of 48 kDa and a minor one of 51 kDa. Western blot and immunoelectron microscopy analyses further showed that the HA107 protein was associated with the nucleocapsids of both budded virions (BVs) and occlusion-derived virions. A knockout virus expressing enhanced green fluorescent protein and polyhedrin was constructed using bacmid technology. A one-step virus growth curve indicated that the BV titre of the knockout virus was significantly higher than that of the parental virus and a repair virus. Bioassays indicated that the knockout virus was able to infect third-instar larvae; however, its median lethal dose (LD) was significantly higher than those of the parental virus and repair virus. These data indicate that encodes a non-essential structural protein of HearNPV virions and that deletion of this gene increases the BV titre and LD of the occluded virus.

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2007-12-01
2019-11-18
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References

  1. Appel, R. D., Baroch, A. & Hochstrasser, D. F. ( 1994; ). A new generation of information retrieval tools for biologists: the example of the ExPASy WWW server. Trends Biochem Sci 19, 258–260.[CrossRef]
    [Google Scholar]
  2. Bulach, D. M., Kumar, C. A., Zaia, A., Liang, B. & Tribe, D. E. ( 1999; ). Group II nucleopolyhedrovirus subgroups revealed by phylogenetic analysis of polyhedrin and DNA polymerase gene sequences. J Invertebr Pathol 73, 59–73.[CrossRef]
    [Google Scholar]
  3. Chen, X., IJkel, W. F. J., Tarchini, R., Sun, X., Sandbrink, H., Wang, H., Peters, S., Zuidema, D., Lankhorst, R. K. & other authors ( 2001; ). The sequence of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus genome. J Gen Virol 82, 241–257.
    [Google Scholar]
  4. Chen, X., Zhang, W.-J., Wong, J., Chun, G., Lu, A., McCutchen, B. F., Presnail, J. K., Herrmann, R., Dolan, M. & other authors ( 2002; ). Comparative analysis of the complete genome sequences of Helicoverpa zea and Helicoverpa armigera single-nucleocapsid nucleopolyhedroviruses. J Gen Virol 83, 673–684.
    [Google Scholar]
  5. Datsenko, K. A. & Wendell, B. ( 2000; ). One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 97, 6640–6645.[CrossRef]
    [Google Scholar]
  6. Friesen, P. D. & Miller, L. K. ( 1985; ). Temporal regulation of baculovirus RNA: overlapping early and late transcripts. J Virol 54, 392–400.
    [Google Scholar]
  7. Funk, C. J., Braunagel, S. C. & Rohrmann, G. F. ( 1997; ). Baculovirus structure. In The Baculoviruses, pp. 7–32. Edited by L. K. Miller. New York: Plenum Press.
  8. Guan, K. L. & Dixon, J. E. ( 1991; ). Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase. Anal Biochem 192, 262–267.[CrossRef]
    [Google Scholar]
  9. Herniou, E. A., Olszewski, J. A., Cory, J. S. & O'Reilly, D. R. ( 2003; ). The genome sequence and evolution of baculoviruses. Annu Rev Entomol 48, 211–234.[CrossRef]
    [Google Scholar]
  10. Horton, P. & Nakai, K. ( 1997; ). Better prediction of protein cellular localization sites with the k nearest neighbors classifier. Proc Int Conf Intell Syst Mol Biol 5, 147–152.
    [Google Scholar]
  11. Hou, S., Chen, X., Wang, H., Tao, M. & Hu, Z. ( 2002; ). Efficient method to generate homologous recombinant baculovirus genomes in E. coli. Biotechniques 32, 783–788.
    [Google Scholar]
  12. IJkel, W. F., van Strien, E. A., Heldens, J. G., Broer, R., Zuidema, D., Goldbach, R. W. & Vlak, J. M. ( 1999; ). Sequence and organization of the Spodoptera exigua multicapsid nucleopolyhedrovirus genome. J Gen Virol 80, 3289–3304.
    [Google Scholar]
  13. Jakubowska, A. K., Peters, S. A., Ziemnicka, J., Vlak, J. M. & van Oers, M. M. ( 2006; ). Genome sequence of an enhancin gene-rich nucleopolyhedrovirus (NPV) from Agrotis segetum: collinearity with Spodoptera exigua multiple NPV. J Gen Virol 87, 537–551.[CrossRef]
    [Google Scholar]
  14. Jensen, L. J., Gupta, R., Staerfeldt, H. H. & Brunak, S. ( 2003; ). Prediction of human protein function according to gene ontology categories. Bioinformatics 19, 635–642.[CrossRef]
    [Google Scholar]
  15. Long, G., Chen, X., Peters, D., Vlak, J. M. & Hu, Z. ( 2003; ). Open reading frame 122 of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus encodes a novel structural protein of occlusion-derived virions. J Gen Virol 84, 115–121.[CrossRef]
    [Google Scholar]
  16. McIntosh, A. H., Christian, P. D. & Grasela, J. J. ( 1999; ). The establishment of heliothine cell lines and their susceptibility to two baculovirus. In Vitro Cell Dev Biol 35, 94–97.
    [Google Scholar]
  17. Pang, Y., Yu, J., Wang, L., Hu, X., Bao, W., Li, G., Chen, C., Han, H., Hu, S. & Yang, H. ( 2001; ). Sequence analysis of the Spodoptera litura multicapsid nucleopolyhedrovirus genome. Virology 287, 391–404.[CrossRef]
    [Google Scholar]
  18. Shizuya, H., Birren, B., Kim, U. J., Mancino, V., Slepak, T., Tachiiri, Y. & Simon, M. ( 1992; ). Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proc Natl Acad Sci U S A 89, 8794–8797.[CrossRef]
    [Google Scholar]
  19. Snedecor, G. W. & Cochran, W. G. ( 1989; ). Statistical Methods, 8th edn. Ames: Iowa State University Press.
  20. Sun, X., Zhang, G., Zhang, Z., Hu, Z. H., Vlak, J. M. & Arif, B. M. ( 1998; ). In vivo cloning of Helicoverpa armigera single nucleocapsid nuclear polyhedrosis virus genotypes. Virol Sin 13, 83–88.
    [Google Scholar]
  21. Theilmann, D. A., Blissard, G. W., Bonning, B., Jehle, J. A., O'Reilly, D. R., Rohrmann, G. F., Thiem, S. & Vlak, J. M. ( 2004; ). Baculoviridae. In VIIIth Report of the International Committee on Taxonomy of Viruses, pp. 177–185. Edited by C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger & L. A. Ball. San Diego: Academic Press.
  22. Tsai, J. M., Wang, H. C., Leu, J. H., Wang, A. H., Zhuang, Y., Walker, P. J., Kou, G. H. & Lo, C. F. ( 2006; ). Identification of the nucleocapsid, tegument, and envelope proteins of the shrimp white spot syndrome virus virion. J Virol 80, 3021–3029.[CrossRef]
    [Google Scholar]
  23. Volkman, L. E. & Summers, M. D. ( 1977; ). Autographa californica nuclear polyhedrosis virus: comparative infectivity of the occluded, alkali-liberated, and nonoccluded forms. J Invertebr Pathol 30, 102–103.[CrossRef]
    [Google Scholar]
  24. Wang, H., Deng, F., Pijlman, G. P., Chen, X., Sun, X., Vlak, J. M. & Hu, Z. ( 2003; ). Cloning of biologically active genomes from a Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus isolate by using a bacterial artificial chromosome. Virus Res 97, 57–63.[CrossRef]
    [Google Scholar]
  25. Zanotto, P. M., Kessing, B. D. & Maruniak, J. E. ( 1993; ). Phylogenetic interrelationships among baculoviruses: evolutionary rates and host associations. J Invertebr Pathol 62, 147–164.[CrossRef]
    [Google Scholar]
  26. Zhang, G. Y. ( 1989; ). Commercial viral insecticide-Heliothis armigera viral insecticide in China. IPM Pract 11, 13
    [Google Scholar]
  27. Zhang, C. X., Ma, X. C. & Guo, Z. J. ( 2005; ). Comparison of the complete genome sequence between C1 and G4 isolates of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus. Virology 333, 190–199.[CrossRef]
    [Google Scholar]
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