1887

Abstract

The open reading frame of single nucleocapsid nucleopolyhedrovirus (HearNPV) encodes a putative protein of 51 kDa with homologues in a few group II NPVs and a granulovirus. was transcribed as polyadenylated transcripts in infected HzAM1 insect cells. The transcripts were initiated at two distinct locations, one upstream of (superoxide dismutase gene, ) and the second upstream of . By Western blot analysis, two forms of the HA107 protein were detected in infected cells, a major polypeptide of 48 kDa and a minor one of 51 kDa. Western blot and immunoelectron microscopy analyses further showed that the HA107 protein was associated with the nucleocapsids of both budded virions (BVs) and occlusion-derived virions. A knockout virus expressing enhanced green fluorescent protein and polyhedrin was constructed using bacmid technology. A one-step virus growth curve indicated that the BV titre of the knockout virus was significantly higher than that of the parental virus and a repair virus. Bioassays indicated that the knockout virus was able to infect third-instar larvae; however, its median lethal dose (LD) was significantly higher than those of the parental virus and repair virus. These data indicate that encodes a non-essential structural protein of HearNPV virions and that deletion of this gene increases the BV titre and LD of the occluded virus.

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2007-12-01
2021-01-24
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