1887

Abstract

A truncated structural protein of hepatitis E virus (HEV), p239, occurs as 23 nm particles consisting of partial homodimers. As the latter resemble the HEV capsomere structurally and antigenically, it was postulated that the recombinant protein may serve as a probe for the HEV receptor. This hypothesis was supported by findings that purified p239 bound and penetrated different cell lines that are susceptible to HEV, and inhibited HEV infection of these cells. The binding was blocked by four of six monoclonal antibodies (mAbs) reactive against the dimeric domain of p239, and by two of three mAbs reactive against its monomeric domain, suggesting that binding may involve a portion of each domain. Mutation affecting the monomeric domain had no effect on binding or capacity to block HEV infection, whereas that affecting the dimeric domain diminished binding of the mutant peptide markedly and abrogated its capacity to block HEV infection. These results suggest that HEV infection might involve distinct receptor-binding sites.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.83308-0
2008-01-01
2020-01-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/89/1/245.html?itemId=/content/journal/jgv/10.1099/vir.0.83308-0&mimeType=html&fmt=ahah

References

  1. Emerson, S. U., Nguyen, H., Graff, J., Stephany, D. A., Brockington, A. & Purcell, R. H. ( 2004; ). In vitro replication of hepatitis E virus (HEV) genomes and of an HEV replicon expressing green fluorescent protein. J Virol 78, 4838–4846.[CrossRef]
    [Google Scholar]
  2. Huang, R., Nakazono, N., Ishii, K., Li, D., Kawamata, O., Kawaguchi, R. & Tsukada, Y. ( 1995; ). Hepatitis E virus (87A strain) propagated in A549 cells. J Med Virol 47, 299–302.[CrossRef]
    [Google Scholar]
  3. Li, S. W., Zhang, J., He, Z. Q., Gu, Y., Liu, R. S., Lin, J., Chen, Y. X., Ng, M. H. & Xia, N. S. ( 2005a; ). Mutational analysis of essential interactions involved in the assembly of hepatitis E virus capsid. J Biol Chem 280, 3400–3406.[CrossRef]
    [Google Scholar]
  4. Li, S. W., Zhang, J., Li, Y. M., Ou, S. H., Huang, G. Y., He, Z. Q., Ge, S. X., Xian, Y. L., Pang, S. Q. & other authors ( 2005b; ). A bacterially expressed particulate hepatitis E vaccine: antigenicity, immunogenicity and protectivity on primates. Vaccine 23, 2893–2901.[CrossRef]
    [Google Scholar]
  5. Meng, J., Dubreuil, P. & Pillot, J. ( 1997; ). A new PCR-based seroneutralization assay in cell culture for diagnosis of hepatitis E. J Clin Microbiol 35, 1373–1377.
    [Google Scholar]
  6. Purcell, R. H. & Emerson, S. U. ( 2001; ). Animal models of hepatitis A and E. ILAR J 42, 161–177.[CrossRef]
    [Google Scholar]
  7. Tam, A. W., Smith, M. M., Guerra, M. E., Huang, C. C., Bradley, D. W., Fry, K. E. & Reyes, G. R. ( 1991; ). Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome. Virology 185, 120–131.[CrossRef]
    [Google Scholar]
  8. Tanaka, T., Takahashi, M., Kusano, E. & Okamoto, H. ( 2007; ). Development and evaluation of an efficient cell-culture system for Hepatitis E virus. J Gen Virol 88, 903–911.[CrossRef]
    [Google Scholar]
  9. Wu, T., Wu, X. L., Ou, S. H., Lin, C. X., Cheng, T., Li, S. W., Ng, M. H., Zhang, J. & Xia, N. S. ( 2007; ). Difference of T cell and B cell activation in two homologous proteins with similar antigenicity but great distinct immunogenicity. Mol Immunol 44, 3261–3266.[CrossRef]
    [Google Scholar]
  10. Xing, L., Kato, K., Li, T., Takeda, N., Miyamura, T., Hammar, L. & Cheng, R. H. ( 1999; ). Recombinant hepatitis E capsid protein self-assembles into a dual-domain T=1 particle presenting native virus epitopes. Virology 265, 35–45.[CrossRef]
    [Google Scholar]
  11. Zhang, J., Ge, S. X., Huang, G. Y., Li, S. W., He, Z. Q., Wang, Y. B., Zheng, Y. J., Gu, Y., Ng, M. H. & Xia, N. S. ( 2003; ). Evaluation of antibody-based and nucleic acid-based assays for diagnosis of hepatitis E virus infection in a rhesus monkey model. J Med Virol 71, 518–526.[CrossRef]
    [Google Scholar]
  12. Zhang, J., Gu, Y., Ge, S. X., Li, S. W., He, Z. Q., Huang, G. Y., Zhuang, H., Ng, M. H. & Xia, N. S. ( 2005; ). Analysis of hepatitis E virus neutralization sites using monoclonal antibodies directed against a virus capsid protein. Vaccine 23, 2881–2892.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.83308-0
Loading
/content/journal/jgv/10.1099/vir.0.83308-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error