1887

Abstract

Deformed wing virus (DWV) induces wing deformation when bees are infected during their pupal development. Field observations and laboratory experiments suggest that the mite is a vector of the virus. Moreover, it has been stated that DWV replicates within this mite. In order to understand the role of in the transmission of DWV, the objective of this work was to locate the sites of retention and/or replication of DWV within the mite by immunohistochemistry. There was no evidence that DWV was replicating in the mite as no tissues showed specific antibody binding to DWV. Also, there were no specific structures that could be suggested as retention sites. DWV was found only in the midgut lumen of in structures resembling large, dense spheres, which were presumably faecal pellets.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.83223-0
2008-07-01
2019-11-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/89/7/1685.html?itemId=/content/journal/jgv/10.1099/vir.0.83223-0&mimeType=html&fmt=ahah

References

  1. Allen, M. F. & Ball, B. V. ( 1996; ). The incidence and world distribution of honey bee viruses. Bee World 77, 141–162.[CrossRef]
    [Google Scholar]
  2. Ball, B. V. ( 1989; ). Varroa jacobsoni as a virus vector. In Present Status of Varroatosis in Europe and Progress in the Varroa Mite Control, pp. 241–244. Proceedings of a Meeting of the EC Experts' Group, Udine, Italy.
  3. Ball, B. V. ( 1993; ). The damaging effects of Varroa jacobsoni infestation. In Living with Varroa, pp. 9–16. Proceedings of the International Bee Research Association Symposium. Edited by A. Matheson. London: IBRA Cardiff.
  4. Bowen-Walker, P. L., Martin, S. J. & Gunn, A. ( 1999; ). The transmission of deformed wing virus between honey bees (Apis mellifera L.) by the ectoparasitic mite Varroa jacobsoni Oud. J Invertebr Pathol 73, 101–106.[CrossRef]
    [Google Scholar]
  5. Calderon, R. F., Van Veen, J., Garce, H. & Esquivel, M. E. ( 2003; ). Presence of deformed wing virus and Kashmir bee virus in Africanized honey bee colonies in Costa Rica infested with Varroa destructor. Bee World 84, 112–116.[CrossRef]
    [Google Scholar]
  6. Chen, Y., Smith, I. B., Jr, Collins, A. M., Pettis, J. S. & Feldlaufer, M. F. ( 2004; ). Detection of deformed wing virus infection in honey bees, Apis mellifera L., in the United States. Am Bee J 144, 557–559.
    [Google Scholar]
  7. Chen, Y. P., Higgins, J. A. & Feldlaufer, M. F. ( 2005; ). Quantitative real-time reverse transcription-PCR analysis of deformed wing virus infection in the honeybee (Apis mellifera L.). Appl Environ Microbiol 71, 436–441.[CrossRef]
    [Google Scholar]
  8. De Jong, D., De Jong, P. H. & Goncalves, L. S. ( 1982; ). Weight loss and other damage to developing worker honeybees from infestation with Varroa jacobsoni. J Apic Res 21, 165–167.
    [Google Scholar]
  9. Evans, G. O. ( 1992; ). Principles of Acarology. Wallingford, UK: CAB International.
  10. Kulincevic, J. M., Ball, B. & Mladjan, V. ( 1990; ). Viruses in honey bee colonies infested with Varroa jacobsoni: first findings in Yugoslavia. Acta Vet 40, 37–42.
    [Google Scholar]
  11. Lanzi, G., de Miranda, J. R., Boniotti, M. B., Cameron, C. E., Lavazza, A., Capucci, L., Camazine, S. M. & Rossi, C. ( 2006; ). Molecular and biological characterization of deformed wing virus of honey bees (Apis mellifera L.). J Virol 80, 4998–5009.[CrossRef]
    [Google Scholar]
  12. Martin, S., Hogarth, A., van Breda, J. & Perrett, J. ( 1998; ). A scientific note on Varroa jacobsoni Oudemans and the collapse of Apis mellifera L. colonies in the United Kingdom. Apidologie 29, 369–370.[CrossRef]
    [Google Scholar]
  13. Nault, L. R. ( 1997; ). Arthropod transmission of plant viruses: a new synthesis. Ann Entomol Soc Am 90, 521–541.[CrossRef]
    [Google Scholar]
  14. Nordstrom, S. ( 2003; ). Distribution of deformed wing virus within honey bee (Apis mellifera) brood cells infested with the ectoparasitic mite Varroa destructor. Exp Appl Acarol 29, 293–302.[CrossRef]
    [Google Scholar]
  15. Nuttall, P. A., Jones, L. D., Labuda, M. & Kaufman, W. R. ( 1994; ). Adaptations of arboviruses to ticks. J Med Entomol 31, 1–9.[CrossRef]
    [Google Scholar]
  16. Ongus, J. R., Peters, D., Bonmatin, J., Bengsch, E., Vlak, J. M. & Oers, M. ( 2004; ). Complete sequence of a picorna-like virus of the genus Iflavirus replicating in the mite Varroa destructor. J Gen Virol 85, 3747–3755.[CrossRef]
    [Google Scholar]
  17. Rehacek, J. ( 1965; ). Development of animal viruses and rickettsiae in ticks and mites. Annu Rev Entomol 10, 1–24.[CrossRef]
    [Google Scholar]
  18. Santillán-Galicia, M. T. ( 2006; ). Characterisation of deformed wing and slow paralysis virus transmission by Varroa destructor. PhD thesis, University of Nottingham, UK.
  19. Tentcheva, D., Gauthier, L., Jouve, S., Canabady-Rochelle, L., Dainat, B., Cousserans, F., Colin, M. E., Ball, B. V. & Bergoin, M. ( 2004; ). Polymerase chain reaction detection of deformed wing virus (DWV) in Apis mellifera and Varroa destructor. Apidologie 35, 431–439.[CrossRef]
    [Google Scholar]
  20. Tentcheva, D., Gauthier, L., Bagny, L., Fievet, J., Dainat, B., Cousserans, F., Colin, M. E. & Bergoin, M. ( 2006; ). Comparative analysis of deformed wing virus (DWV) RNA in Apis mellifera and Varroa destructor. Apidologie 37, 41–50.[CrossRef]
    [Google Scholar]
  21. Wiegers, F. P. ( 1986; ). Transmission of honey bee viruses by Varroa jacobsoni Oud. In European Research on Varroatosis Control, pp. 99–104. Edited by R. Cavalloro. Rotterdam: A. A. Balkema.
  22. Yue, C. & Genersch, E. ( 2005; ). RT-PCR analysis of deformed wing virus in honeybees (Apis mellifera) and mites (Varroa destructor). J Gen Virol 86, 3419–3424.[CrossRef]
    [Google Scholar]
  23. Zhang, Q., Ongus, J. R., Boot, W. J., Calis, J., Bonmatin, J. M., Bengsch, E. & Peters, D. ( 2007; ). Detection and localisation of picorna-like virus particles in tissues of Varroa destructor, an ectoparasite of the honey bee, Apis mellifera. J Invertebr Pathol 96, 97–105.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.83223-0
Loading
/content/journal/jgv/10.1099/vir.0.83223-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error