1887

Abstract

During an investigation of arboviruses in China, a novel densovirus (DNV) was isolated from the adult female . The virus, designated densovirus (CppDNV), caused cytopathic effect in C6/36 cells. The virus particles were icosahedral, non-enveloped and had a mean diameter of 24 nm. The complete coding region of CppDNV was found to be 3335 nt and it contained three open reading frames (ORFs). CppDNV shares 82–93 % identical nucleotides with isolates of the densovirus [isolates AalDNV-1, AalDNV-2 (C6/36 DNV) and AalDNV-3], densovirus (AaeDNV) and densovirus (HeDNV). The nucleotide sequence identity among CppDNV isolates exceeds 98 %. Phylogenetic trees based on non-structural (NS1 and NS2) and capsid (VP) genes show that CppDNV clustered with the species AaeDNV and represents a novel variant of this species within the genus .

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2008-01-01
2020-01-27
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Supplements

vol. , part 1, pp. 195 - 199

Densoviruses used in this study

Primers used for sequence amplification of CppDNV

Percentage sequence identity among amino acid sequence of VP (a), NS1 (b) and NS2 (c) of CppDNV [Single PDF file](129 KB)



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