1887

Abstract

Adenovirus infection subverts nucleolar structure and function. B23 is a nucleolar protein present in two isoforms (B23.1 and B23.2) and both isoforms have been identified as stimulatory factors for adenovirus DNA replication. Here, it is demonstrated that the two isoforms of B23, B23.1 and B23.2, interact and co-localize differently with viral DNA replication proteins pTP and DBP in adenovirus-infected cells. Thus, the mechanism by which the two proteins stimulate viral DNA replication is likely to differ. These data also demonstrate the importance of testing both isoforms of B23 for interactions with viral proteins and nucleic acids.

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2007-12-01
2019-11-20
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References

  1. Bevington, J. M., Needham, P. G., Verrill, K. C., Collaco, R. F., Basrur, V. & Trempe, J. P. ( 2007; ). Adeno-associated virus interactions with B23/nucleophosmin: identification of sub-nucleolar virion regions. Virology 357, 102–113.[CrossRef]
    [Google Scholar]
  2. Castiglia, C. L. & Flint, S. J. ( 1983; ). Effects of adenovirus infection on rRNA synthesis and maturation in HeLa cells. Mol Cell Biol 3, 662–671.
    [Google Scholar]
  3. Chang, J. H. & Olson, M. O. ( 1989; ). A single gene codes for two forms of rat nucleolar protein B23 mRNA. J Biol Chem 264, 11732–11737.
    [Google Scholar]
  4. Chen, D. & Huang, S. ( 2001; ). Nucleolar components involved in ribosome biogenesis cycle between the nucleolus and nucleoplasm in interphase cells. J Cell Biol 153, 169–176.[CrossRef]
    [Google Scholar]
  5. de Jong, R. N., Meijer, L. A. & van der Vliet, P. C. ( 2003a; ). DNA binding properties of the adenovirus DNA replication priming protein pTP. Nucleic Acids Res 31, 3274–3286.[CrossRef]
    [Google Scholar]
  6. de Jong, R. N., van der Vliet, P. C. & Brenkman, A. B. ( 2003b; ). Adenovirus DNA replication: protein priming, jumping back and the role of the DNA binding protein DBP. Curr Top Microbiol Immunol 272, 187–211.
    [Google Scholar]
  7. Fankhauser, C., Izaurralde, E., Adachi, Y., Wingfield, P. & Laemmli, U. K. ( 1991; ). Specific complex of human immunodeficiency virus type 1 rev and nucleolar B23 proteins: dissociation by the Rev response element. Mol Cell Biol 11, 2567–2575.
    [Google Scholar]
  8. Flint, J. & Shenk, T. ( 1997; ). Viral transactivating proteins. Annu Rev Genet 31, 177–212.[CrossRef]
    [Google Scholar]
  9. Lawrence, F. J., McStay, B. & Matthews, D. A. ( 2006; ). Nucleolar protein upstream binding factor is sequestered into adenovirus DNA replication centres during infection without affecting RNA polymerase I location or ablating rRNA synthesis. J Cell Sci 119, 2621–2631.[CrossRef]
    [Google Scholar]
  10. Liu, H., Naismith, J. H. & Hay, R. T. ( 2003; ). Adenovirus DNA replication. Curr Top Microbiol Immunol 272, 131–164.
    [Google Scholar]
  11. Lutz, P., Puvion-Dutilleul, F., Lutz, Y. & Kedinger, C. ( 1996; ). Nucleoplasmic and nucleolar distribution of the adenovirus IVa2 gene product. J Virol 70, 3449–3460.
    [Google Scholar]
  12. Mai, R. T., Yeh, T. S., Kao, C. F., Sun, S. K., Huang, H. H. & Wu Lee, Y. H. ( 2006; ). Hepatitis C virus core protein recruits nucleolar phosphoprotein B23 and coactivator p300 to relieve the repression effect of transcriptional factor YY1 on B23 gene expression. Oncogene 25, 448–462.
    [Google Scholar]
  13. Matthews, D. A. ( 2001; ). Adenovirus protein V induces redistribution of nucleolin and B23 from nucleolus to cytoplasm. J Virol 75, 1031–1038.[CrossRef]
    [Google Scholar]
  14. Monaghan, A., Webster, A. & Hay, R. T. ( 1994; ). Adenovirus DNA binding protein: helix destabilising properties. Nucleic Acids Res 22, 742–748.[CrossRef]
    [Google Scholar]
  15. Okuwaki, M., Iwamatsu, A., Tsujimoto, M. & Nagata, K. ( 2001; ). Identification of nucleophosmin/B23, an acidic nucleolar protein, as a stimulatory factor for in vitro replication of adenovirus DNA complexed with viral basic core proteins. J Mol Biol 311, 41–55.[CrossRef]
    [Google Scholar]
  16. Okuwaki, M., Tsujimoto, M. & Nagata, K. ( 2002; ). The RNA binding activity of a ribosome biogenesis factor, nucleophosmin/B23, is modulated by phosphorylation with a cell cycle-dependent kinase and by association with its subtype. Mol Biol Cell 13, 2016–2030.[CrossRef]
    [Google Scholar]
  17. Temperley, S. M. & Hay, R. T. ( 1992; ). Recognition of the adenovirus type 2 origin of DNA replication by the virally encoded DNA polymerase and preterminal proteins. EMBO J 11, 761–768.
    [Google Scholar]
  18. Tsuda, Y., Mori, Y., Abe, T., Yamashita, T., Okamoto, T., Ichimura, T., Moriishi, K. & Matsuura, Y. ( 2006; ). Nucleolar protein B23 interacts with Japanese encephalitis virus core protein and participates in viral replication. Microbiol Immunol 50, 225–234.[CrossRef]
    [Google Scholar]
  19. Walton, T. H., Moen, P. T., Jr, Fox, E. & Bodnar, J. W. ( 1989; ). Interactions of minute virus of mice and adenovirus with host nucleoli. J Virol 63, 3651–3660.
    [Google Scholar]
  20. Wang, D., Umekawa, H. & Olson, M. O. ( 1993; ). Expression and subcellular locations of two forms of nucleolar protein B23 in rat tissues and cells. Cell Mol Biol Res 39, 33–42.
    [Google Scholar]
  21. Webster, A., Leith, I. R. & Hay, R. T. ( 1994; ). Activation of adenovirus-coded protease and processing of preterminal protein. J Virol 68, 7292–7300.
    [Google Scholar]
  22. Webster, A., Leith, I. R. & Hay, R. T. ( 1997; ). Domain organization of the adenovirus preterminal protein. J Virol 71, 539–547.
    [Google Scholar]
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