@article{mbs:/content/journal/jgv/10.1099/vir.0.83107-0, author = "Fischer, Matthew A. and Tscharke, David C. and Donohue, Keri B. and Truckenmiller, Mary E. and Norbury, Christopher C.", title = "Reduction of vector gene expression increases foreign antigen-specific CD8+ T-cell priming", journal= "Journal of General Virology", year = "2007", volume = "88", number = "9", pages = "2378-2386", doi = "https://doi.org/10.1099/vir.0.83107-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.83107-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Viral vectors have been shown to induce protective CD8+ T-cell populations in animal models, but significant obstacles remain to their widespread use for human vaccination. One such obstacle is immunodominance, where the CD8+ T-cell response to a vector can suppress the desired CD8+ T-cell response to a recombinantly encoded antigen. To overcome this hurdle, we broadly reduced vector-specific gene expression. We treated a recombinant vaccinia virus, encoding antigen as a minimal peptide determinant (8–10 aa), with psoralen and short-wave UV light. The resulting virus induced 66 % fewer vector-specific immunodominant CD8+ T cells, allowing the in vivo induction of an increased number of CD8+ T cells specific for the recombinant antigen.", }