1887

Abstract

Viral vectors have been shown to induce protective CD8 T-cell populations in animal models, but significant obstacles remain to their widespread use for human vaccination. One such obstacle is immunodominance, where the CD8 T-cell response to a vector can suppress the desired CD8 T-cell response to a recombinantly encoded antigen. To overcome this hurdle, we broadly reduced vector-specific gene expression. We treated a recombinant vaccinia virus, encoding antigen as a minimal peptide determinant (8–10 aa), with psoralen and short-wave UV light. The resulting virus induced 66 % fewer vector-specific immunodominant CD8 T cells, allowing the induction of an increased number of CD8 T cells specific for the recombinant antigen.

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2007-09-01
2019-10-17
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