1887

Abstract

Nectin-1 is an alphaherpesvirus receptor that binds to virion glycoprotein D by the first immunoglobulin (Ig)-like domain. The possibility of making animals resistant to pseudorabies virus (PRV) infection has been investigated by generating transgenic mice expressing soluble forms of porcine nectin-1. Previously, transgenic mice were generated that expressed a fusion protein made of the entire ectodomain of nectin-1 fused to the Fc portion of human IgG, or the first Ig-like domain fused to the Fc portion of porcine IgG. Here, the contribution of the second and third Ig-like domains of nectin-1 was analysed by generating transgenic mice expressing the entire ectodomain of nectin-1 fused to the porcine Fc portion. Transgenic mice expressing each of three different fusion proteins were challenged with PRV for comparison of their resistance. Altogether, mice transgenic for a chimera that carried the entire ectodomain were more resistant than those transgenic for a chimera that carried the first Ig-like domain.

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2007-10-01
2021-07-26
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References

  1. Cai W., Gu B., Person S. 1988; Role of glycoprotein B of herpes simplex virus type 1 in viral entry and cell fusion. J Virol 62:2596–2604
    [Google Scholar]
  2. Campadelli-Fiume G., Cocchi F., Menotti L., Lopez M. 2000; The novel receptors that mediate the entry of herpes simplex viruses and animal alphaherpesviruses into cells. Rev Med Virol 10:305–319 [CrossRef]
    [Google Scholar]
  3. Cocchi F., Lopez M., Menotti L., Aoubala M., Dubreuil P., Campadelli-Fiume G. 1998a; The V domain of herpesvirus Ig-like receptor (HigR) contains a major functional region in herpes simplex virus-1 entry into cells and interacts physically with the viral glycoprotein D. Proc Natl Acad Sci U S A 95:15700–15705 [CrossRef]
    [Google Scholar]
  4. Cocchi F., Menotti L., Mirandola P., Lopez M., Campadelli-Fiume G. 1998b; The ectodomain of a novel member of the immunoglobulin subfamily related to the poliovirus receptor has the attribute of a bona fide receptor for herpes simplex virus types 1 and 2 in human cells. J Virol 72:9992–10002
    [Google Scholar]
  5. Cocchi F., Lopez M., Dubreuil P., Campadelli-Fiume G., Menotti L. 2001; Chimeric nectin 1-poliovirus receptor molecules identify a nectin 1 region functional in herpes simplex virus entry. J Virol 75:7987–7994 [CrossRef]
    [Google Scholar]
  6. Fabre S., Reymond N., Cocchi F., Menotti L., Dubreuil P., Campadelli-Fiume G., Lopez M. 2002; Prominent role of the Ig-like V domain in trans-interactions of nectins. Nectin3 and nectin 4 bind to the predicted C–C′-C″-D β -strands of the nectin1 V domain. J Biol Chem 277:27006–27013 [CrossRef]
    [Google Scholar]
  7. Forrester A., Farrell H., Wilkinson G., Kaye J., Davis-Poynter N., Minson T. 1992; Construction and properties of a mutant of herpes simplex virus type 1 with glycoprotein H coding sequence deleted. J Virol 66:341–348
    [Google Scholar]
  8. Geraghty R. J., Krummenacher C., Eisenberg R. J., Cohen G. H., Spear P. G. 1998; Entry of alphaherpesviruses mediated by poliovirus receptor related protein 1 and polioviris receptor. Science 280:1618–1620 [CrossRef]
    [Google Scholar]
  9. Herold B. C., Visalli R. J., Susmarski N., Brandt C. R., Spear P. G. 1994; Glycoprotein C-independent binding of herpes simplex virus to cells requires cell surface heparan sulphate and glycoprotein B. J Gen Virol 75:1211–1222 [CrossRef]
    [Google Scholar]
  10. Krummenacher C., Rux A. H., Whitbeck J. C., Ponce de Leon M., Lou H., Baribaud I., Hou W., Zou C., Geraghty R. J. other authors 1999; The first immunoglobulin-like domain of HveC is sufficient to bind herpes simplex virus glycoprotein D with full affinity, while the third domain is involved in oligomerization of HveC. J Virol 73:8127–8137
    [Google Scholar]
  11. Krummenacher C., Baribaud I., Ponce de Leon M., Whitbeck J. C., Lou H., Cohen G. H., Eisenberg R. J. 2000; Localization of a binding site for herpes simplex virus glycoprotein D on herpesvirus entry mediator C by using antireceptor monoclonal antibodies. J Virol 74:10863–10872 [CrossRef]
    [Google Scholar]
  12. Krummenacher C., Baribaud I., Sanzo J. F., Cohen G. H., Eisenberg R. J. 2002; Effects of herpes simplex virus on structure and function of nectin-1/HveC. J Virol 76:2424–2433 [CrossRef]
    [Google Scholar]
  13. Ligas M. W., Johnson D. C. 1988; A herpes simplex virus mutant in which glycoprotein D sequences are replaced by β -galactosidase sequences binds to but is unable to penetrate into cells. J Virol 62:1486–1494
    [Google Scholar]
  14. Mettenleiter T. C., Zsak L., Zuckermann F., Sugg N., Kern H., Ben-Porat T. 1990; Interaction of glycoprotein gIII with a cellular heparin-like substance mediates adsorption of pseudorabies virus. J Virol 64:278–286
    [Google Scholar]
  15. Miyahara M., Nakanishi H., Takahashi K., Satoh-Horikawa K., Tachibana K., Takai Y. 2000; Interaction of nectin with afadin is necessary for its clustering at cell-cell contact sites but not for its cis dimerization or trans interaction. J Biol Chem 275:613–618 [CrossRef]
    [Google Scholar]
  16. Momose Y., Honda T., Inagaki M., Shimizu K., Irie K., Nakanishi H., Takai Y. 2002; Role of the second immunoglobulin-like loop of nectin in cell-cell adhesion. Biochem Biophys Res Commun 293:45–49 [CrossRef]
    [Google Scholar]
  17. Niwa H., Yamamura K., Miyazaki J. 1991; Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193–200 [CrossRef]
    [Google Scholar]
  18. Ono E., Amagai K., Taharaguchi S., Tomioka Y., Yoshino S., Watanabe Y., Cherel P., Houdebine L.-M., Adam M. other authors 2004a; Transgenic mice expressing a soluble form of porcine nectin-1/HveC as a model for pseudorabies-resistant livestock. Proc Natl Acad Sci U S A 101:16150–16155 [CrossRef]
    [Google Scholar]
  19. Ono E., Yoshino S., Amagai K., Taharaguchi S., Kimura C., Morimoto J., Inobe M., Uenishi T., Uede T. 2004b; Enhanced resistance to herpes simplex virus type 1 infection in transgenic mice expressing a soluble form of herpesvirus entry mediator. Virology 320:267–275 [CrossRef]
    [Google Scholar]
  20. Ono E., Tomioka Y., Watanabe Y., Amagai K., Taharaguchi S., Glenisson J., Cherel P. 2006; The first immunoglobulin-like domain of porcine nectin-1 is sufficient to confer resistance to pseudorabies virus infection in transgenic mice. Arch Virol 151:1827–1839 [CrossRef]
    [Google Scholar]
  21. Roop C., Hutchinson L., Johnson D. C. 1993; A mutant herpes simplex virus type 1 unable to express glycoprotein L cannot enter cells, and its particles lack glycoprotein H. J Virol 67:2285–2297
    [Google Scholar]
  22. Shieh M.-T., WuDunn D., Montgomery R. I., Esko J. D., Spear P. G. 1992; Cell surface receptors for herpes simplex virus are heparan sulfate proteoglycans. J Cell Biol 116:1273–1281 [CrossRef]
    [Google Scholar]
  23. Spear P. G., Eisenberg R. J., Cohen G. H. 2000; Three classes of cell surface receptors for alphaherpesvirus entry. Virology 275:1–8 [CrossRef]
    [Google Scholar]
  24. Struyf F., Plate A. E., Spear P. G. 2005; Deletion of the second immunoglobulin-like domain of nectin-1 alters its intracellular processing and localization and ability to mediate entry of herpes simplex virus. J Virol 79:3841–3845 [CrossRef]
    [Google Scholar]
  25. WuDunn D., Spear P. G. 1989; Initial interaction of herpes simplex virus with cells is binding to heparan sulfate. J Virol 63:52–58
    [Google Scholar]
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