1887

Abstract

is an endoparasitoid of larval stages of , the diamondback moth. For successful parasitization, this parasitoid injects a polydnavirus into its host during oviposition. Here we isolated two genes, which we named BV1 and BV2. BV1 was located on segment CvBV-S5 with a size of 790 bp, while BV2 was located on segment CvBV-S51 with a size of 459 bp. A gene copy of BV2 was found on segment CvBV-S48, which we name BV2’. Gene duplication occurred in both genes, tandem gene duplication for BV1 and segmental duplication for BV2. Gene transcripts of the two genes were detected in hosts as early as 0.5 h post-parasitization (p.p.) and continued to be detected for six days, and tissue-specific expression patterns showed that they could be detected in the haemolymph and brain at 2 h p.p., suggesting that they could participate in early protection of parasitoid eggs from host cellular encapsulation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.82999-0
2007-12-01
2019-11-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/88/12/3317.html?itemId=/content/journal/jgv/10.1099/vir.0.82999-0&mimeType=html&fmt=ahah

References

  1. Beaudoing, E., Freier, S., Wyatt, J. R., Claverie, J. & Gautheret, D. ( 2000; ). Patterns of variant polyadenylation signal usage in human genes. Genome Res 10, 1001–1010.[CrossRef]
    [Google Scholar]
  2. Beckage, N. E., Templeton, T. J., Nielsen, B. D., Cook, D. I. & Stoltz, D. B. ( 1987; ). Parasitism-induced hemolymph polypeptides in Manduca sexta (L.) larvae parasitized by the braconid wasp Cotesia congregata (Say). Insect Biochem 17, 439–455.[CrossRef]
    [Google Scholar]
  3. Beckage, N. E., Tan, F. F., Schleifer, K. W., Lane, R. D. & Cherubin, L. L. ( 1994; ). Characterization and biological effects of Cotesia congregata polydnavirus on host larvae of the tobacco hornworm, Manduca sexta. Arch Insect Biochem Physiol 26, 165–195.[CrossRef]
    [Google Scholar]
  4. Chen, Y. P. & Gundersen-Rindal, D. E. ( 2003; ). Morphological and genomic characterization of the polydnavirus associated with the parasitoid wasp Glyptapanteles indiensis (Hymenoptera: Braconidae). J Gen Virol 84, 2051–2060.[CrossRef]
    [Google Scholar]
  5. Chen, Y. P., Higgins, J. A. & Gundersen-Rindal, D. E. ( 2003; ). Quantitation of a Glyptapanteles indiensis polydnavirus gene expressed in parasitized host, Lymantria dispar, by real-time quantitative RT-PCR. J Virol Methods 114, 125–133.[CrossRef]
    [Google Scholar]
  6. Choi, J. Y., Roh, J. Y., Kang, J. N., Shim, H. J., Woo, S. D., Jin, B. R., Li, M. S. & Je, Y. H. ( 2005; ). Genomic segments cloning and analysis of Cotesia plutellae polydnavirus using plasmid capture system. Biochem Biophys Res Commun 332, 487–493.[CrossRef]
    [Google Scholar]
  7. Choo, K. H., Tan, T. W. & Ranganathan, S. ( 2005; ). SPdb--a signal peptide database. BMC Bioinformatics 6, 249 [CrossRef]
    [Google Scholar]
  8. Espagne, E., Dupuy, C., Huguet, E., Cattolico, L., Provost, B., Martins, N., Poirie, M., Periquet, G. & Drezen, J. M. ( 2004; ). Genome sequence of a polydnavirus: insights into symbiotic virus evolution. Science 306, 286–289.[CrossRef]
    [Google Scholar]
  9. Friedman, R. & Hughes, A. L. ( 2006; ). Pattern of gene duplication in the Cotesia congregata Bracovirus. Infect Genet Evol 6, 315–322.[CrossRef]
    [Google Scholar]
  10. Gundersen-Rindal, D. E. & Pedroni, M. J. ( 2006; ). Characterization and transcriptional analysis of protein tyrosine phosphatase genes and an ankyrin repeat gene of the parasitoid Glyptapanteles indiensis polydnavirus in the parasitized host. J Gen Virol 87, 311–322.[CrossRef]
    [Google Scholar]
  11. Harwood, S. H., Grosovsky, A. J., Cowles, E. A., Davis, J. W. & Beckage, N. E. ( 1994; ). An abundantly expressed hemolymph glycoprotein isolated from newly parasitized Manduca sexta larvae is a polydnavirus gene product. Virology 205, 381–392.[CrossRef]
    [Google Scholar]
  12. Henikoff, S. & Henikoff, J. G. ( 1994; ). Protein family classification based on searching a database of blocks. Genomics 19, 97–107.[CrossRef]
    [Google Scholar]
  13. Hilgarth, R. S. & Webb, B. A. ( 2002; ). Characterization of Campoletis sonorensis ichnovirus segment I genes as members of the repeat element gene family. J Gen Virol 83, 2393–2402.
    [Google Scholar]
  14. Kroemer, G. & Jaattela, M. ( 2005; ). Lysosomes and autophagy in cell death control. Nat Rev Cancer 5, 886–897.[CrossRef]
    [Google Scholar]
  15. Kroemer, J. A. & Webb, B. A. ( 2004; ). Polydnavirus genes and genomes: emerging gene families and new insights into polydnavirus replication. Annu Rev Entomol 49, 431–456.[CrossRef]
    [Google Scholar]
  16. Lapointe, R., Wilson, R., Vilaplana, L., O'Reilly, D. R., Falabella, P., Douris, V., Bernier-Cardou, M., Pennacchio, F., Iatrou, K. & other authors ( 2005; ). Expression of a Toxoneuron nigriceps polydnavirus-encoded protein causes apoptosis-like programmed cell death in lepidopteran insect cells. J Gen Virol 86, 963–971.[CrossRef]
    [Google Scholar]
  17. Lavine, M. D. & Strand, M. R. ( 2002; ). Insect hemocytes and their role in immunity. Insect Biochem Mol Biol 32, 1295–1309.[CrossRef]
    [Google Scholar]
  18. Le, N. T., Asgari, S., Amaya, K., Tan, F. F. & Beckage, N. E. ( 2003; ). Persistence and expression of Cotesia congregata polydnavirus in host larvae of the tobacco hornworm, Manduca sexta. J Insect Physiol 49, 533–543.[CrossRef]
    [Google Scholar]
  19. Lin, Y. S., Huang, Y. T., Chen, P. S., Lin, C. F., Jan, M. S. & Lei, H. Y. ( 1999; ). Requirement of I-E molecule for thymocyte apoptosis induced by staphylococcal enterotoxin B in vivo. Cell Immunol 193, 71–79.[CrossRef]
    [Google Scholar]
  20. Nakai, K. & Horton, P. ( 1999; ). psort: a program for detecting sorting signals in proteins and predicting their subcellular localization. Trends Biochem Sci 24, 34–36.[CrossRef]
    [Google Scholar]
  21. Nielsen, H., Engelbrecht, J., Brunak, S. & von Heijne, G. ( 1997; ). Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng 10, 1–6.[CrossRef]
    [Google Scholar]
  22. Ottesen, E. A., Duke, B. O., Karam, M. & Behbehani, K. ( 1997; ). Strategies and tools for the control/elimination of lymphatic filariasis. Bull World Health Organ 75, 491–503.
    [Google Scholar]
  23. Provost, B., Varricchio, P., Arana, E., Espagne, E., Falabella, P., Huguet, E., La Scaleia, R., Cattolico, L., Poirie, M. & other authors ( 2004; ). Bracoviruses contain a large multigene family coding for protein tyrosine phosphatases. J Virol 78, 13090–13103.[CrossRef]
    [Google Scholar]
  24. Salamov, A. A. & Solovyev, V. V. ( 2000; ). Ab initio gene finding in Drosophila genomic DNA. Genome Res 10, 516–522.[CrossRef]
    [Google Scholar]
  25. Shaw, M. R. ( 2003; ). Revised synonymy in the genus Cotesia (Hymenoptera: Braconidae: Microgastrinae): the identity of Microgaster vestalis Haliday, 1834, as a senior synonym of Apanteles plutellae Kurdjumov, 1912. Entomol Gaz 54, 187–189.
    [Google Scholar]
  26. Solovyev, V. V. & Salamov, A. A. ( 1999; ). INFOGENE: a database of known gene structures and predicted genes and proteins in sequences of genome sequencing projects. Nucleic Acids Res 27, 248–250.[CrossRef]
    [Google Scholar]
  27. Stoltz, D. B. ( 1990; ). Evidence for chromosomal transmission of polydnavirus DNA. J Gen Virol 71, 1051–1056.[CrossRef]
    [Google Scholar]
  28. Stoltz, D. B., Guzo, D. & Cook, D. ( 1986; ). Studies on polydnavirus transmission. Virology 155, 120–131.[CrossRef]
    [Google Scholar]
  29. Strand, M. R. & Pech, L. L. ( 1995; ). Microplitis demolitor polydnavirus induces apoptosis of a specific haemocyte morphotype in Pseudoplusia includens. J Gen Virol 76, 283–291.[CrossRef]
    [Google Scholar]
  30. Strand, M. R., Witherell, R. A. & Trudeau, D. ( 1997; ). Two Microplitis demolitor polydnavirus mRNAs expressed in hemocytes of Pseudoplusia includens contain a common cysteine-rich domain. J Virol 71, 2146–2156.
    [Google Scholar]
  31. Tardy, C., Codogno, P., Autefage, H., Levade, T. & Andrieu-Abadie, N. ( 2006; ). Lysosomes and lysosomal proteins in cancer cell death (new players of an old struggle). Biochim Biophys Acta 1765, 101–125.
    [Google Scholar]
  32. Turnbull, M. & Webb, B. ( 2002; ). Perspectives on polydnavirus origins and evolution. Adv Virus Res 58, 203–254.
    [Google Scholar]
  33. Webb, B. A. & Cui, L. ( 1998; ). Relationships between polydnavirus genomes and viral gene expression. J Insect Physiol 44, 785–793.[CrossRef]
    [Google Scholar]
  34. Whitfield, J. B. ( 1997; ). Molecular and morphological data suggest a single origin of the polydnaviruses among braconid wasps. Naturwissenschaften 84, 502–507.[CrossRef]
    [Google Scholar]
  35. Whitfield, J. B. ( 2002; ). Estimating the age of the polydnavirus/braconid wasp symbiosis. Proc Natl Acad Sci U S A 99, 7508–7513.[CrossRef]
    [Google Scholar]
  36. Yu, R. X., Chen, Y. F., Chen, X. X., Huang, F., Lou, Y. G. & Liu, S. S. ( 2007; ). Effects of venom/calyx fluid from the endoparasitic wasp Cotesia plutellae on the hemocytes of its host Plutella xylostella in vitro. J Insect Physiol 53, 22–29.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.82999-0
Loading
/content/journal/jgv/10.1099/vir.0.82999-0
Loading

Data & Media loading...

Supplements

vol. , part 12, pp. 3317–3322

. Primers used for RT-PCR [PDF](47 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error