1887

Abstract

In this study, kinetics of attachment and internalization of feline infectious peritonitis virus (FIPV) serotype I strain Black and serotype II strain 79-1146 were determined in feline monocytes from two cats and in Crandell feline kidney (CrFK) cells. Attached FIPV I (Black) particles were observed on almost all monocytes. Within 1 h, 17 particles were bound per cell and, within 1 min, 89 % of the bound particles were internalized. For FIPV II (79-1146), attachment was observed on 66 and 95 % of all monocytes from the two cats. After 1 h, respectively five and 20 particles were bound per cell (all cells considered). Within 1 min, 60 % of the bound particles were internalized. Internalization in monocytes was efficient and proceeded via endocytosis. In CrFK cells, attachment and internalization were less efficient, especially for FIPV I (Black), so this cell line is not suitable for studying FIPV entry.

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2007-09-01
2020-01-28
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References

  1. Black, J. W. ( 1980; ). Recovery and in vitro cultivation of a coronavirus from laboratory-induced cases of feline infectious peritonitis (FIP). Vet Med Small Anim Clin 75, 811–814.
    [Google Scholar]
  2. Cavanagh, D. ( 1997; ). Nidovirales: a new order comprising Coronaviridae and Arteriviridae. Arch Virol 142, 629–633.
    [Google Scholar]
  3. Cowley, J. A., Dimmock, C. M., Spann, K. M. & Walker, P. J. ( 2000; ). Gill-associated virus of Penaeus monodon prawns: an invertebrate virus with ORF1a and ORF1b genes related to arteri- and coronaviruses. J Gen Virol 81, 1473–1484.
    [Google Scholar]
  4. Delmas, B., Gelfi, J., L'Haridon, R., Vogel, L. K., Sjöström, H., Norén, O. & Laude, H. ( 1992; ). Aminopeptidase N is a major receptor for the enteropathogenic coronavirus TGEV. Nature 357, 417–419.[CrossRef]
    [Google Scholar]
  5. Delmas, B., Gelfi, J., Sjöström, H., Norén, O. & Laude, H. ( 1993; ). Further characterization of aminopeptidase N as a receptor for coronaviruses. Adv Exp Med Biol 342, 293–298.
    [Google Scholar]
  6. Delmas, B., Gelfi, J., Kut, E., Sjöström, H., Norén, O. & Laude, H. ( 1994; ). Determinants essential for the transmissible gastroenteritis virus-receptor interaction reside within a domain of aminopeptidase N that is distinct from the enzymatic site. J Virol 68, 5216–5224.
    [Google Scholar]
  7. Delputte, P. L. & Nauwynck, H. J. ( 2004; ). Porcine arterivirus infection of alveolar macrophages is mediated by sialic acid on the virus. J Virol 78, 8094–8101.[CrossRef]
    [Google Scholar]
  8. Delputte, P. L., Vanderheijden, N., Nauwynck, H. J. & Pensaert, M. B. ( 2002; ). Involvement of the matrix protein in attachment of porcine reproductive and respiratory syndrome virus to a heparinlike receptor on porcine alveolar macrophages. J Virol 76, 4312–4320.[CrossRef]
    [Google Scholar]
  9. Dewerchin, H. L., Cornelissen, E. & Nauwynck, H. J. ( 2005; ). Replication of feline coronaviruses in peripheral blood monocytes. Arch Virol 150, 2483–2500.[CrossRef]
    [Google Scholar]
  10. Duan, X., Nauwynck, H. J. & Pensaert, M. B. ( 1997; ). Effects of origin and state of differentiation and activation of monocytes/macrophages on their susceptibility to porcine reproductive and respiratory syndrome virus (PRRSV). Arch Virol 142, 2483–2497.[CrossRef]
    [Google Scholar]
  11. González, J. M., Gomez-Puertas, P., Cavanagh, D., Gorbalenya, A. E. & Enjuanes, L. ( 2003; ). A comparative sequence analysis to revise the current taxonomy of the family Coronaviridae. Arch Virol 148, 2207–2235.[CrossRef]
    [Google Scholar]
  12. Gorbalenya, A. E., Enjuanes, L., Ziebuhr, J. & Snijder, E. J. ( 2006; ). Nidovirales: evolving the largest RNA virus genome. Virus Res 117, 17–37.[CrossRef]
    [Google Scholar]
  13. Hansen, G. H., Delmas, B., Besnardeau, L., Vogel, L. K., Sjöström, H., Laude, H. & Norén, O. ( 1998; ). The coronavirus transmissible gastroenteritis virus causes infection after receptor-mediated endocytosis and acid-dependent fusion with an intracellular compartment. J Virol 72, 527–534.
    [Google Scholar]
  14. Hohdatsu, T., Okada, S., Ishizuka, Y., Yamada, H. & Koyoma, H. ( 1992; ). The prevalence of types I and II feline coronavirus infections in cats. J Vet Med Sci 54, 557–562.[CrossRef]
    [Google Scholar]
  15. Hohdatsu, T., Izumiya, Y. & Yokoyama, Y. ( 1998; ). Differences in virus receptor for type I and type II feline infectious peritonitis virus. Arch Virol 143, 839–850.[CrossRef]
    [Google Scholar]
  16. Kooi, C., Cervin, M. & Anderson, R. ( 1991; ). Differentiation of acid-pH-dependent and -nondependent entry pathways for mouse hepatitis virus. Virology 180, 108–119.[CrossRef]
    [Google Scholar]
  17. McKeirnan, A. J., Evermann, J. F., Hargis, A., Miller, L. M. & Ott, R. L. ( 1981; ). Isolation of feline coronaviruses from two cats with diverse disease manifestations. Feline Pract 11, 16–20.
    [Google Scholar]
  18. Morahan, P. S., Connor, J. R. & Leary, K. R. ( 1985; ). Viruses and the versatile macrophage. Br Med Bull 41, 15–21.
    [Google Scholar]
  19. Nash, T. C. & Buchmeier, M. J. ( 1997; ). Entry of mouse hepatitis virus into cells by endosomal and nonendosomal pathways. Virology 233, 1–8.[CrossRef]
    [Google Scholar]
  20. Nauwynck, H. J., Duan, X., Favoreel, H., Van Oostveldt, P. & Pensaert, M. ( 1999; ). Entry of porcine reproductive and respiratory syndrome virus into alveolar macrophages via receptor-mediated endocytosis. J Gen Virol 80, 297–305.
    [Google Scholar]
  21. Nomura, R., Kiyota, A., Suzaki, E., Kataoka, K., Ohe, Y., Miyamoto, K., Senda, T. & Fujimoto, T. ( 2004; ). Human coronavirus 229E binds to CD13 in rafts and enters the cell through caveolae. J Virol 78, 8701–8708.[CrossRef]
    [Google Scholar]
  22. Pedersen, N. C., Black, J. W., Boyle, J. F., Evermann, J. F., McKeirnan, A. J. & Ott, R. L. ( 1983; ). Pathogenic differences between various feline coronavirus isolates. In Molecular Biology and Pathogenesis of Coronaviruses, pp. 365–380. London: Plenum.
  23. Pedersen, N. C., Evermann, J. F., Alison, J., McKeirnan, A. J. & Ott, R. L. ( 1984; ). Pathogenicity studies of feline coronavirus isolates 79-1146 and 79-1683. Am J Vet Res 45, 2580–2585.
    [Google Scholar]
  24. Rottier, P. J. M., Nakamura, K., Schellen, P., Volders, H. & Haijema, B. J. ( 2005; ). Acquisition of macrophage tropism during the pathogenesis of feline infectious peritonitis is determined by mutations in the feline coronavirus spike protein. J Virol 79, 14122–14130.[CrossRef]
    [Google Scholar]
  25. Savarino, A., Boelaert, J. R., Cassone, A., Majori, G. & Cauda, R. ( 2003; ). Effects of chloroquine on viral infections: an old drug against today's diseases? Lancet Infect Dis 3, 722–727.[CrossRef]
    [Google Scholar]
  26. Spaan, W. J. M., Brian, D., Cavanagh, D., de Groot, R. J., Enjuanes, L., Gorbalenya, A. E., Holmes, K. V., Masters, P. S., Rottier, P. & other authors ( 2005; ). Family Coronaviridae. In Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses, pp. 947–964. Edited by C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger & L. A. Ball. San Diego, CA: Elsevier Academic Press.
  27. Stoddart, C. A. & Scott, F. W. ( 1989; ). Intrinsic resistance of feline infectious peritoneal macrophages to coronavirus infection correlates with in vivo virulence. J Virol 63, 436–440.
    [Google Scholar]
  28. Tresnan, D. B., Levis, R. & Holmes, K. V. ( 1996; ). Feline aminopeptidase N serves as a receptor for feline, canine, porcine and human coronaviruses in serogroup I. J Virol 70, 8669–8674.
    [Google Scholar]
  29. Vennema, H., Poland, A., Floyd Hawkins, K. & Pedersen, N. C. ( 1995; ). A comparison of the genomes of FECVs and FIPVs and what they tell us about the relationships between feline coronaviruses and their evolution. Feline Pract 23, 40–44.
    [Google Scholar]
  30. Yeager, C. L., Ashmun, R. A., Williams, R. K., Cardellichio, C. B., Shapiro, L. H., Look, A. T. & Holmes, K. V. ( 1992; ). Human aminopeptidase N is a receptor for human coronavirus 229E. Nature 357, 420–422.[CrossRef]
    [Google Scholar]
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