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Journal of General Virology header logo Journal of General Virology

Volume 89, Issue 4

Characterization of interleukin-1 mRNA expression in chicken macrophages in response to avian reovirus No Access

Abstract

Inhibitors of viral disassembly or RNA and protein synthesis, viral disassembly intermediates (infectious subviral particles, ISVP), binary ethylenimine-inactivated virions, and viral particles lacking genomic double-stranded (ds) RNA (empty particles) were used to assess the expression of interleukin-1 (IL-1) mRNA in chicken (chIL-1) macrophages in response to avian reovirus. The results demonstrate that two distinct expression patterns of chIL-1 mRNA mediated by different steps in viral replication were found. Viral disassembly was required for the induction of a rapid, transient expression pattern of chIL-1 mRNA that was rapidly induced at 30 min, with maximal levels reached by 2 h, and fell to a low level within 6 h post-inoculation, while viral RNA synthesis rather than protein translation, which was subsequent to membrane penetration, was required to induce a stable, sustained expression pattern of chIL-1 mRNA that occurred at and after 6 h post-inoculation. In addition, the induction of chIL-1 mRNA expression by the empty particles and ISVP was extremely weak, compared with the active dsRNA virions or binary ethylenimine-inactivated virions, suggesting that the presence of dsRNA, even if transcriptionally inactive, may be an important factor in this response.

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2008-04-01
2025-03-24
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/content/journal/jgv/10.1099/vir.0.82957-0
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Characterization of interleukin-1β mRNA expression in chicken macrophages in response to avian reovirus
J. Gen. Virol. 89, 1059 (2008); https://doi.org/10.1099/vir.0.82957-0
/content/journal/jgv/10.1099/vir.0.82957-0
/content/journal/jgv/10.1099/vir.0.82957-0
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