1887

Abstract

The delayed-early DNA polymerase promoter of Chilo iridescent virus (CIV), officially known as , was fine mapped by constructing a series of increasing deletions and by introducing point mutations. The effects of these mutations were examined in a luciferase reporter gene system using cells transfected with promoter constructs and infected with CIV. When the size of the upstream element was reduced from position −19 to −15, relative to the transcriptional start site, the luciferase activity was reduced to almost zero. Point mutations showed that each of the 5 nt (AAAAT) located between –19 and –15 were equally essential for promoter activity. Mutations at individual bases around the transcription initiation site showed that the promoter extended until position −2 upstream of the transcription start site. South-Western analysis showed that a protein of approximately 100 kDa interacted with the −19 nt promoter fragment in CIV-infected cells. This binding did not occur with a point mutant that lacked promoter activity. The AAAAT motif was also found in the DNA polymerase promoter region of other iridoviruses and in other putative CIV delayed-early genes.

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2007-09-01
2024-03-29
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References

  1. Barray S., Devauchelle G. 1987; Protein synthesis in cells infected by Chilo iridescent virus: evidence for temporal control of three classes of induced polypeptides. Virology 138:253–261
    [Google Scholar]
  2. Beckman W., Tham T. N., Aubertin A. M., Willis D. B. 1988; Structure and regulation of the immediate-early frog virus 3 gene that encodes ICR489. J Virol 62:1271–1277
    [Google Scholar]
  3. Blissard G. W., Kogan P. H., Wei R., Rohrmann G. F. 1992; A synthetic early promoter from a baculovirus: roles of the TATA box and conserved start site CAGT sequence in basal levels of transcription. Virology 190:783–793 [CrossRef]
    [Google Scholar]
  4. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72:248–254 [CrossRef]
    [Google Scholar]
  5. Cerutti M., Cerutti P., Devauchelle G. 1989; Infectivity of vesicles prepared from Chilo iridescent virus inner membrane: evidence for recombination between associated DNA fragments. Virus Res 12:299–313 [CrossRef]
    [Google Scholar]
  6. D'Costa S. M., Yao H., Bilimoria S. L. 2001; Transcription and temporal cascade in Chilo iridescent virus infected cells. Arch Virol 146:2165–2178 [CrossRef]
    [Google Scholar]
  7. D'Costa S. M., Yao H. J., Bilimoria S. L. 2004; Transcriptional mapping in Chilo iridescent virus infections. Arch Virol 149:723–742 [CrossRef]
    [Google Scholar]
  8. Darai G., Anders K., Koch H. G., Delius H., Gelderblom H., Samalecos C., Flügel R. M. 1983; Analysis of the genome of fish lymphocystis disease virus isolated directly from epidermal tumours of pleuronectes. Virology 126:466–479 [CrossRef]
    [Google Scholar]
  9. Darai G., Delius H., Clarke J., Apfel H., Schnitzler P., Flugel R. M. 1985; Molecular cloning and physical mapping of the genome of fish lymphocystis disease virus. Virology 146:292–301 [CrossRef]
    [Google Scholar]
  10. Delhon G., Tulman E. R., Afonso C. L., Lu Z., Becnel J. J., Moser B. A., Kutish G. F., Rock D. L. 2006; Genome of invertebrate iridescent virus type 3 (mosquito iridescent virus). J Virol 80:8439–8449 [CrossRef]
    [Google Scholar]
  11. Delius H., Darai G., Flügel R. M. 1984; DNA analysis of insect iridescent virus 6: evidence for circular permutation and terminal redundancy. J Virol 49:609–614
    [Google Scholar]
  12. Fauquet C. M., Mayo M. A., Maniloff J., Desselberger U., Ball L. A. (editors) 2005 Virus Taxonomy : Eighth Report of the International Committee for Virus Taxonomy. San Diego, CA: Elsevier Academic Press;
    [Google Scholar]
  13. Goorha R. 1981; Frog virus 3 requires RNA polymerase II for its replication. J Virol 37:496–499
    [Google Scholar]
  14. Goorha R. 1982; Frog virus 3 DNA replication occurs in two stages. J Virol 43:519–528
    [Google Scholar]
  15. Goorha R., Murti K. G. 1982; The genome of frog virus 3, an animal DNA virus, is circularly permuted and terminally redundant. Proc Natl Acad Sci U S A 79:248–252 [CrossRef]
    [Google Scholar]
  16. Goorha R., Murti G., Granoff A., Tirey R. 1978; Macromolecular synthesis in cells infected by frog virus 3. VIII. The nucleus is a site of frog virus 3 DNA and RNA synthesis. Virology 84:32–50 [CrossRef]
    [Google Scholar]
  17. Huang W. M., Buchanan J. M. 1974; Synergistic interactions of T4 early proteins concerned to DNA. Proc Natl Acad Sci U S A 71:2226–2230 [CrossRef]
    [Google Scholar]
  18. Jakob N. J., Muller K., Bahr U., Darai G. 2001; Analysis of the first complete DNA sequence of an invertebrate iridovirus: coding strategy of the genome of Chilo iridescent virus. Virology 286:182–196 [CrossRef]
    [Google Scholar]
  19. Jarvis D. L., Weinkauf C., Guarino L. A. 1996; Immediate-early baculovirus vectors for foreign gene expression in transformed or infected insect cells. Protein Expr Purif 8:191–203 [CrossRef]
    [Google Scholar]
  20. Lua D. T., Yasuike M., Hirono I., Aoki T. 2005; Transcription program of red sea bream iridovirus as revealed by DNA microarrays. J Virol 79:15151–15164 [CrossRef]
    [Google Scholar]
  21. Marina C. F., Arredondo-Jimenez J. I., Castillo A., Williams T. 1999; Sublethal effects of iridovirus disease in a mosquito. Oecologia 119:383–388 [CrossRef]
    [Google Scholar]
  22. Nalçacioğlu R., Demirbağ Z., Vlak J. M., van Oers M. M. 2003; Promoter analysis of the Chilo iridescent virus DNA polymerase and major capsid protein genes. Virology 317:321–329 [CrossRef]
    [Google Scholar]
  23. Pallister J., Goldie S., Coupar B., Hyatt A. 2005; Promoter activity in the 5′ flanking regions of the Bohle iridovirus ICP 18, ICP 46 and major capsid protein genes. Arch Virol 150:1911–1919 [CrossRef]
    [Google Scholar]
  24. Williams T., Barbosa-Solomieu V., Chincar V. G. 2005; A decade of advances in iridovirus research. Adv Virus Res 65:173–248
    [Google Scholar]
  25. Willis D. B. 1987; DNA sequences required for trans activation of an immediate- early frog virus 3 gene. Virology 161:1–7 [CrossRef]
    [Google Scholar]
  26. Willis D. B., Granoff A. 1985; Transactivation of an immediate-early frog virus 3 promoter by a virion protein. J Virol 56:495–501
    [Google Scholar]
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