RT Journal Article SR Electronic(1) A1 Nerome, Reiko A1 Tajima, Shigeru A1 Takasaki, Tomohiko A1 Yoshida, Takashi A1 Kotaki, Akira A1 Lim, Chang-Kweng A1 Ito, Mikako A1 Sugiyama, Akira A1 Yamauchi, Akinori A1 Yano, Takuya A1 Kameyama, Taeko A1 Morishita, Ichiko A1 Kuwayama, Masaru A1 Ogawa, Tomoko A1 Sahara, Keiji A1 Ikegaya, Asaka A1 Kanda, Masahiro A1 Hosoya, Yoshiyuki A1 Itokazu, Kiyomasa A1 Onishi, Hajime A1 Chiya, Seizou A1 Yoshida, Yasuko A1 Tabei, Yukiko A1 Katsuki, Kazuko A1 Tabata, Koji A1 Harada, Seiya A1 Kurane, IchiroYR 2007 T1 Molecular epidemiological analyses of Japanese encephalitis virus isolates from swine in Japan from 2002 to 2004 JF Journal of General Virology, VO 88 IS 10 SP 2762 OP 2768 DO https://doi.org/10.1099/vir.0.82941-0 PB Microbiology Society, SN 1465-2099, AB To characterize Japanese encephalitis virus (JEV) strains recently prevalent in Japan, JEV surveillance was performed in pigs from 2002 to 2004. Eleven new JEV isolates were obtained and compared with previous isolates from Japan and other Asian countries. All of the isolates were classified into genotype 1 by nucleotide sequence analysis of the E gene. Two new isolates with different levels of neurovirulence and neuroinvasiveness, but with only one nucleotide difference in the E gene, Sw/Mie/34/2004 and Sw/Mie/40/2004, were isolated at the same farm on the same day. Sw/Mie/40/2004 displayed higher neurovirulence and neuroinvasiveness in mice than the other four new isolates. Another new isolate, Sw/Hiroshima/25/2002, was neutralized by antiserum to Beijing-1 at a level similar to the homologous Beijing-1 strain, whilst seven other new isolates were neutralized at 10-fold-lower titres. However, there were no amino acid differences in the E protein among these eight isolates. The present study indicated that the 11 new JEV isolates were genetically similar, but biologically and serologically heterogeneous., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.82941-0