1887

Abstract

To characterize Japanese encephalitis virus (JEV) strains recently prevalent in Japan, JEV surveillance was performed in pigs from 2002 to 2004. Eleven new JEV isolates were obtained and compared with previous isolates from Japan and other Asian countries. All of the isolates were classified into genotype 1 by nucleotide sequence analysis of the E gene. Two new isolates with different levels of neurovirulence and neuroinvasiveness, but with only one nucleotide difference in the E gene, Sw/Mie/34/2004 and Sw/Mie/40/2004, were isolated at the same farm on the same day. Sw/Mie/40/2004 displayed higher neurovirulence and neuroinvasiveness in mice than the other four new isolates. Another new isolate, Sw/Hiroshima/25/2002, was neutralized by antiserum to Beijing-1 at a level similar to the homologous Beijing-1 strain, whilst seven other new isolates were neutralized at 10-fold-lower titres. However, there were no amino acid differences in the E protein among these eight isolates. The present study indicated that the 11 new JEV isolates were genetically similar, but biologically and serologically heterogeneous.

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2007-10-01
2024-12-12
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References

  1. Ali A., Igarashi A. 1997; Antigenic and genetic variations among Japanese encephalitis virus strains belonging to genotype 1. Microbiol Immunol 41:241–252 [CrossRef]
    [Google Scholar]
  2. Chen W. R., Tesh R. B., Rico-Hesse R. 1990; Genetic variation of Japanese encephalitis virus in nature. J Gen Virol 71:2915–2922 [CrossRef]
    [Google Scholar]
  3. Gritsun T. S., Venugopal K., Zanotto P. M., Mikhailov M. V., Sall A. A., Holmes E. C., Polkinghorne I., Frolova T. V., Pogodina V. V. other authors 1997; Complete sequence of two tick-borne flaviviruses isolated from Siberia and the UK: analysis and significance of the 5′ and 3′-UTRs. Virus Res 49:27–39 [CrossRef]
    [Google Scholar]
  4. Hanna J. N., Ritchie S. A., Phillips D. A., Shield J., Bailey M. C., Mackenzie J. S., Poidinger M., McCall B. J., Mills P. J. 1996; An outbreak of Japanese encephalitis in the Torres Strait. Australia; 1995 Med J Aust 165:256–260
    [Google Scholar]
  5. Hasegawa H., Yoshida M., Fujita S., Kobayashi Y. 1994; Comparison of structural proteins among antigenically different Japanese encephalitis virus strains. Vaccine 12:841–844 [CrossRef]
    [Google Scholar]
  6. Hashimoto H., Nomoto A., Watanabe K., Mori T., Takezawa T., Aizawa C., Takegami T., Hiramatsu K. 1988; Molecular cloning and complete nucleotide sequence of the genome of Japanese encephalitis virus Beijing-1 strain. Virus Genes 1:305–317 [CrossRef]
    [Google Scholar]
  7. Infectious Diseases Surveillance Center; 2004; Japanese encephalitis. In National Epidemiological Surveillance of Vaccine-Preventable Diseases pp 92–113 Tokyo: NIID;
    [Google Scholar]
  8. Ito M., Takasaki T., Yamada K., Nerome R., Tajima S., Kurane I. 2004; Development and evaluation of fluorogenic TaqMan reverse transcriptase PCR assays for detection of dengue virus types 1 to 4. J Clin Microbiol 42:5935–5937 [CrossRef]
    [Google Scholar]
  9. Kuwayama M., Ito M., Takao S., Shimazu Y., Fukuda S., Miyazaki K., Kurane I., Takasaki T. 2005; Japanese encephalitis virus in meningitis patients, Japan. Emerg Infect Dis 11:471–473
    [Google Scholar]
  10. Ma S. P., Yoshida Y., Makino Y., Tadano M., Ono T., Ogawa M. 2003; Short report: a major genotype of Japanese encephalitis virus currently circulating in Japan. Am J Trop Med Hyg 69:151–154
    [Google Scholar]
  11. Mangada M. N., Takegami T. 1999; Molecular characterization of the Japanese encephalitis virus representative immunotype strain JaGAr 01. Virus Res 59:101–112 [CrossRef]
    [Google Scholar]
  12. McMinn P. C. 1997; The molecular basis of virulence of the encephalitogenic flaviviruses. J Gen Virol 78:2711–2722
    [Google Scholar]
  13. Nam J. H., Chung Y. J., Ban S. J., Kim E. J., Park Y. K., Cho H. W. 1996; Envelope gene sequence variation among Japanese encephalitis viruses isolated in Korea. Acta Virol 40:303–309
    [Google Scholar]
  14. Nga P. T., del Carmen Parquet M., Cuong V. D., Ma S. P., Hasebe F., Inoue S., Makino Y., Takagi M., Nam V. S., Morita K. 2004; Shift in Japanese encephalitis virus (JEV) genotype circulating in northern Vietnam: implications for frequent introductions of JEV from Southeast Asia to East Asia. J Gen Virol 85:1625–1631 [CrossRef]
    [Google Scholar]
  15. Paranjpe S., Banerjee K. 1996; Phylogenetic analysis of the envelope gene of Japanese encephalitis virus. Virus Res 42:107–117 [CrossRef]
    [Google Scholar]
  16. Pyke A. T., Williams D. T., Nisbet D. J., van den Hurk A. F., Taylor C. T., Johansen C. A., Macdonald J., Hall R. A., Simmons R. J. other authors 2001; The appearance of a second genotype of Japanese encephalitis virus in the Australasian region. Am J Trop Med Hyg 65:747–753
    [Google Scholar]
  17. Reed L. J., Muench H. 1938; A simple method of estimating fifty per cent endpoints. Am J Hyg 27:493–497
    [Google Scholar]
  18. Rey F. A., Heinz F. X., Mandl C., Kunz C., Harrison S. C. 1995; The envelope glycoprotein from tick-borne encephalitis virus at 2 Å resolution. Nature 375:291–298 [CrossRef]
    [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  20. Solomon T., Ni H., Beasley D. W., Ekkelenkamp M., Cardosa M. J., Barrett A. D. 2003; Origin and evolution of Japanese encephalitis virus in southeast Asia. J Virol 77:3091–3098 [CrossRef]
    [Google Scholar]
  21. Spicer P. E., Phillips D., Pike A., Johansen C., Melrose W., Hall R. A. 1999; Antibodies to Japanese encephalitis virus in human sera collected from Irian Jaya. Follow-up of a previously reported case of Japanese encephalitis in that region. Trans R Soc Trop Med Hyg 93:511–514 [CrossRef]
    [Google Scholar]
  22. Sumiyoshi H., Mori C., Fuke I., Morita K., Kuhara S., Kondou J., Kikuchi Y., Nagamatu H., Igarashi A. 1987; Complete nucleotide sequence of the Japanese encephalitis virus genome RNA. Virology 161:497–510 [CrossRef]
    [Google Scholar]
  23. Takegami T. 2003; Japanese encephalitis. Uirusu 53:25–30 (in Japanese [CrossRef]
    [Google Scholar]
  24. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  25. Tsai T. F. 2000; New initiatives for the control of Japanese encephalitis by vaccination: minutes of a WHO/CVI meeting. Bangkok, Thailand; 13–15 October 1998. Vaccine 18Suppl. 21–25 [CrossRef]
    [Google Scholar]
  26. Williams D. T., Wang L. F., Daniels P. W., Mackenzie J. S. 2000; Molecular characterization of the first Australian isolate of Japanese encephalitis virus, the FU strain. J Gen Virol 81:2471–2480
    [Google Scholar]
  27. WHO 1998; Japanese encephalitis vaccine. Wkly Epidemiol Rec 73:337–344
    [Google Scholar]
  28. Yang D. K., Kim B. H., Kweon C. H., Kwon J. H., Lim S. I., Han H. R. 2004; Molecular characterization of full-length genome of Japanese encephalitis virus (KV1899) isolated from pigs in Korea. J Vet Sci 5:197–205
    [Google Scholar]
  29. Yoshida Y., Tabei Y., Hasegawa M., Nagashima M., Morozumi S. 2005; Genotypic analysis of Japanese encephalitis virus strains isolated from swine in Tokyo, Japan. Jpn J Infect Dis 58:259–261
    [Google Scholar]
  30. Zhao Z., Date T., Li Y., Kato T., Miyamoto M., Yasui K., Wakita T. 2005; Characterization of the E-138 (Glu/Lys) mutation in Japanese encephalitis virus by using a stable, full-length, infectious cDNA clone. J Gen Virol 86:2209–2220 [CrossRef]
    [Google Scholar]
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