RT Journal Article SR Electronic(1) A1 Milbradt, Jens A1 Auerochs, Sabrina A1 Marschall, ManfredYR 2007 T1 Cytomegaloviral proteins pUL50 and pUL53 are associated with the nuclear lamina and interact with cellular protein kinase C JF Journal of General Virology, VO 88 IS 10 SP 2642 OP 2650 DO https://doi.org/10.1099/vir.0.82924-0 PB Microbiology Society, SN 1465-2099, AB Human cytomegalovirus-encoded pUL50 and pUL53 belong to a group of conserved herpesviral nuclear proteins. This study describes: (i) the co-localization of pUL50 with components of the nuclear lamina such as lamins A/C and lamin B receptor by double immunofluorescent staining, (ii) a strong pUL50-mediated relocalization of pUL53 from a diffuse nuclear pattern towards a nuclear rim localization, (iii) a direct interaction between pUL50 and pUL53, as well as between pUL50 and protein kinase C (PKC), shown by yeast two-hybrid and co-immunoprecipitation analyses, (iv) in vitro phosphorylation of pUL50, which is highly suggestive of PKC activity, and finally (v) partial relocalization of PKC by pUL50/pUL53 from its main cytoplasmic localization to a marked nuclear lamina accumulation. These data suggest a role for pUL50 and pUL53 in the recruitment of PKC, an event that is considered to be important for cytomegalovirus-induced distortion of the nuclear lamina., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.82924-0