1887

Abstract

The protective immune response to African swine fever virus (ASFV) includes both cellular and serological components. In this study, the role of antibodies in the pathogenicity and diagnosis of African swine fever (ASF) was explored. Accordingly, total and Ig isotype antibody responses against the 12 viral proteins previously demonstrated to be the main targets of serological immunity were evaluated in longitudinally collected sera from pigs infected experimentally with the non-pathogenic ASFV/NH/P68 isolate. Strong total IgG antibody responses were observed against viral proteins E183L/p54, K205R/‘unassigned’, A104R/histone-like and B602L/‘unassigned’; therefore, IgM, IgG1 and IgG2 responses to these proteins were also determined. One protein stimulating IgM (K205R) may have practical potential for the detection of recently infected animals. There was a clear trend towards an IgG1 response to all of the proteins. This may reflect a dominant Th2-controlled immune response. In order to identify possible correlations between these serological responses and the pathogenesis of ASF, total IgG responses to the 12 recombinant proteins were compared in asymptomatic and chronically infected animals. For the proteins NP419L/DNA ligase, CP312R, B646L/p73, K196R/thymidine kinase and K205R, the antibody titres were significantly higher in animals developing lesions. One exception was the antibody response to the A104R/histone-like protein, which was higher in asymptomatic than in chronically infected pigs, suggesting that antibodies against this protein might be an indicator of an effective immune response or that this response is somehow involved in protection.

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2007-09-01
2020-10-21
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References

  1. Aguero, M., Fernandez, J., Romero, L., Sanchez Mascaraque, C., Arias, M. & Sanchez-Vizcaino, J. M. ( 2003; ). Highly sensitive PCR assay for routine diagnosis of African swine fever virus in clinical samples. J Clin Microbiol 41, 4431–4434.[CrossRef]
    [Google Scholar]
  2. Barderas, M. G., Wigdorovitz, A., Merelo, F., Beitia, F., Alonso, C., Borca, M. V. & Escribano, J. M. ( 2000; ). Serodiagnosis of African swine fever using the recombinant protein p30 expressed in insect larvae. J Virol Methods 89, 129–136.[CrossRef]
    [Google Scholar]
  3. Crawley, A. & Wilkie, B. N. ( 2003; ). Porcine Ig isotypes: function and molecular characteristics. Vaccine 21, 2911–2922.[CrossRef]
    [Google Scholar]
  4. Crawley, A., Raymond, C. & Wilkie, B. N. ( 2003; ). Control of immunoglobulin isotype production by porcine B-cells cultured with cytokines. Vet Immunol Immunopathol 91, 141–154.[CrossRef]
    [Google Scholar]
  5. Dixon, L. K., Escribano, J. M., Martins, C., Rock, D. L., Salas, M. L. & Wilkinson, P. J. ( 2005; ). Asfarviridae. In Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses, pp. 135–143. Edited by C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger & L. A. Ball. London: Elsevier/Academic Press.
  6. Gallardo, C., Blanco, E., Rodriguez, J. M., Carrascosa, A. L. & Sanchez-Vizcaino, J. M. ( 2006; ). Antigenic properties and diagnostic potential of African swine fever virus protein pp62 expressed in insect cells. J Clin Microbiol 44, 950–956.[CrossRef]
    [Google Scholar]
  7. Gómez-Puertas, P., Rodriguez, F., Oviedo, J. M., Ramiro-Ibanez, F., Ruiz-Gonzalvo, F., Alonso, C. & Escribano, J. M. ( 1996; ). Neutralizing antibodies to different proteins of African swine fever virus inhibit both virus attachment and internalization. J Virol 70, 5689–5694.
    [Google Scholar]
  8. Gómez-Puertas, P., Rodriguez, F., Oviedo, J. M., Brun, A., Alonso, C. & Escribano, J. M. ( 1998; ). The African swine fever virus proteins p54 and p30 are involved in two distinct steps of virus attachment and both contribute to the antibody-mediated protective immune response. Virology 243, 461–471.[CrossRef]
    [Google Scholar]
  9. Hjertner, B., Meehan, B., McKillen, J., McNeilly, F. & Belak, S. ( 2005; ). Adaptation of an Invader assay for the detection of African swine fever virus DNA. J Virol Methods 124, 1–10.[CrossRef]
    [Google Scholar]
  10. Jenson, J. S., Childerstone, A., Takamatsu, H., Dixon, L. K. & Parkhouse, R. M. ( 2000; ). The cellular immune recognition of proteins expressed by an African swine fever virus random genomic library. J Immunol Methods 242, 33–42.[CrossRef]
    [Google Scholar]
  11. Kollnberger, S. D., Gutierrez-Castaneda, B., Foster-Cuevas, M., Corteyn, A. & Parkhouse, R. M. ( 2002; ). Identification of the principal serological immunodeterminants of African swine fever virus by screening a virus cDNA library with antibody. J Gen Virol 83, 1331–1342.
    [Google Scholar]
  12. Leitão, A., Cartaxeiro, C., Coelho, R., Cruz, B., Parkhouse, R. M., Portugal, F., Vigario, J. D. & Martins, C. L. ( 2001; ). The non-haemadsorbing African swine fever virus isolate ASFV/NH/P68 provides a model for defining the protective anti-virus immune response. J Gen Virol 82, 513–523.
    [Google Scholar]
  13. Malmquist, W. A. ( 1963; ). Serologic and immunologic studies with African swine fever virus. Am J Vet Res 24, 450–459.
    [Google Scholar]
  14. Martins, C. L., Lawman, M. J., Scholl, T., Mebus, C. A. & Lunney, J. K. ( 1993; ). African swine fever virus specific porcine cytotoxic T cell activity. Arch Virol 129, 211–225.[CrossRef]
    [Google Scholar]
  15. Neilan, J. G., Lu, Z., Kutish, G. F., Sussman, M. D., Roberts, P. C., Yozawa, T. & Rock, D. L. ( 1993; ). An African swine fever virus gene with similarity to bacterial DNA binding proteins, bacterial integration host factors, and the Bacillus phage SPO1 transcription factor, TF1. Nucleic Acids Res 21, 1496 [CrossRef]
    [Google Scholar]
  16. Neilan, J. G., Zsak, L., Lu, Z., Burrage, T. G., Kutish, G. F. & Rock, D. L. ( 2004; ). Neutralizing antibodies to African swine fever virus proteins p30, p54, and p72 are not sufficient for antibody-mediated protection. Virology 319, 337–342.[CrossRef]
    [Google Scholar]
  17. OIE ( 2004; ). African swine fever. In Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, 5th edn, p. 1178. Paris: Office International de Epizooties.
  18. Onisk, D. V., Borca, M. V., Kutish, G., Kramer, E., Irusta, P. & Rock, D. L. ( 1994; ). Passively transferred African swine fever virus antibodies protect swine against lethal infection. Virology 198, 350–354.[CrossRef]
    [Google Scholar]
  19. Oura, C. A., Denyer, M. S., Takamatsu, H. & Parkhouse, R. M. ( 2005; ). In vivo depletion of CD8+ T lymphocytes abrogates protective immunity to African swine fever virus. J Gen Virol 86, 2445–2450.[CrossRef]
    [Google Scholar]
  20. Oviedo, J. M., Rodriguez, F., Gomez-Puertas, P., Brun, A., Gomez, N., Alonso, C. & Escribano, J. M. ( 1997; ). High level expression of the major antigenic African swine fever virus proteins p54 and p30 in baculovirus and their potential use as diagnostic reagents. J Virol Methods 64, 27–35.[CrossRef]
    [Google Scholar]
  21. Pérez-Filgueira, D. M., Gonzalez-Camacho, F., Gallardo, C., Resino-Talavan, P., Blanco, E., Gomez-Casado, E., Alonso, C. & Escribano, J. M. ( 2006; ). Optimization and validation of recombinant serological tests for African swine fever diagnosis based on detection of the p30 protein produced in Trichoplusia ni larvae. J Clin Microbiol 44, 3114–3121.[CrossRef]
    [Google Scholar]
  22. Stevens, T. L., Bossie, A., Sanders, V. M., Fernandez-Botran, R., Coffman, R. L., Mosmann, T. R. & Vitetta, E. S. ( 1988; ). Regulation of antibody isotype secretion by subsets of antigen-specific helper T cells. Nature 334, 255–258.[CrossRef]
    [Google Scholar]
  23. Vidal, M. I., Stiene, M., Henkel, J., Bilitewski, U., Costa, J. V. & Oliva, A. G. ( 1997; ). A solid-phase enzyme linked immunosorbent assay using monoclonal antibodies, for the detection of African swine fever virus antigens and antibodies. J Virol Methods 66, 211–218.[CrossRef]
    [Google Scholar]
  24. Wardley, R. C., Norley, S. G., Wilkinson, P. J. & Williams, S. ( 1985; ). The role of antibody in protection against African swine fever virus. Vet Immunol Immunopathol 9, 201–212.[CrossRef]
    [Google Scholar]
  25. Zsak, L., Onisk, D. V., Afonso, C. L. & Rock, D. L. ( 1993; ). Virulent African swine fever virus isolates are neutralized by swine immune serum and by monoclonal antibodies recognizing a 72-kDa viral protein. Virology 196, 596–602.[CrossRef]
    [Google Scholar]
  26. Zsak, L., Borca, M. V., Risatti, G. R., Zsak, A., French, R. A., Lu, Z., Kutish, G. F., Neilan, J. G., Callahan, J. D. & other authors ( 2005; ). Preclinical diagnosis of African swine fever in contact-exposed swine by a real-time PCR assay. J Clin Microbiol 43, 112–119.[CrossRef]
    [Google Scholar]
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