RT Journal Article SR Electronic(1) A1 Jung, Jin Kyu A1 Kwun, Hyun Jin A1 Lee, Jung-Ok A1 Arora, Payal A1 Jang, Kyung LibYR 2007 T1 Hepatitis B virus X protein differentially affects the ubiquitin-mediated proteasomal degradation of β-catenin depending on the status of cellular p53 JF Journal of General Virology, VO 88 IS 8 SP 2144 OP 2154 DO https://doi.org/10.1099/vir.0.82836-0 PB Microbiology Society, SN 1465-2099, AB Abnormal accumulation of β-catenin is considered to be a strong driving force in hepatocellular carcinogenesis; however, the mechanism of β-catenin accumulation in tumours is unclear. Here, it was demonstrated that hepatitis B virus X protein (HBx) differentially regulates the level of β-catenin through two ubiquitin-dependent proteasome pathways depending on p53 status. In the presence of p53, HBx downregulated β-catenin through the activation of a p53–Siah-1 proteasome pathway. For this purpose, HBx upregulated Siah-1 expression at the transcriptional level via activation of p53. In the absence of p53, however, HBx stabilized β-catenin through the inhibition of a glycogen synthase kinase-3β-dependent pathway. Interestingly, HBx variants with a Pro-101 to Ser substitution were unable to activate p53 and thus could stabilize β-catenin irrespective of p53 status. Based on these findings, a model of β-catenin regulation by HBx is proposed whereby the balance between the two opposite activities of HBx determines the overall expression level of β-catenin. Differential regulation of β-catenin by HBx depending on host (p53 status) and viral factors (HBx sequence variation) helps not only to explain the observation that cancers accumulating β-catenin also exhibit a high frequency of p53 mutations but also to understand the contradictory reports on the roles of HBx during hepatocellular carcinogenesis., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.82836-0