@article{mbs:/content/journal/jgv/10.1099/vir.0.82830-0, author = "Tiwari, Vaibhav and Shukla, Shripaad Y. and Yue, Beatrice Y. J. T. and Shukla, Deepak", title = "Herpes simplex virus type 2 entry into cultured human corneal fibroblasts is mediated by herpesvirus entry mediator", journal= "Journal of General Virology", year = "2007", volume = "88", number = "8", pages = "2106-2110", doi = "https://doi.org/10.1099/vir.0.82830-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.82830-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Herpes simplex virus type 2 (HSV-2) infections in the eye are becoming increasingly common in adults. The most likely point of entry for HSV-2 into the eye is through the cornea. By using primary cultures of human corneal fibroblasts (CFs), a natural target-cell type for infection, it was demonstrated that CFs are highly susceptible to HSV-2 entry and replication. RT-PCR and flow-cytometry analyses demonstrated expression of herpesvirus entry mediator (HVEM), a known mediator for HSV-2 entry into cells. Blocking of virus entry into CFs by anti-HVEM antibody implicated HVEM as a potential receptor for HSV-2 infection. These results indicate that HVEM may play a crucial role in HSV-2-induced corneal infections.", }