1887

Abstract

Earlier reports of a human exogenous retrovirus (HMTV) related closely to mouse mammary tumor virus (MMTV) led us to search for these viral sequences in breast cancer tissues and normal tissues. A real-time PCR was developed based on MMTV and published HMTV envelope sequences. The real-time PCR method can detect one to ten copies of MMTV target DNA. Tissue samples were collected prospectively from 18 breast cancer patients and 11 non-malignant control cases, as well as peripheral blood leukocytes from the same women. Despite the high sensitivity of the real-time PCR method used, none of the samples were positive for HMTV DNA or RNA. The absence of HMTV DNA in both breast cancer samples and controls indicates either that the concentration of putative HMTV DNA in the breast cancers was too low for detection or that it did not exist there.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.82767-0
2007-06-01
2019-12-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/88/6/1806.html?itemId=/content/journal/jgv/10.1099/vir.0.82767-0&mimeType=html&fmt=ahah

References

  1. Andersson, M. L., Medstrand, P., Yin, H. & Blomberg, J. ( 1996; ). Differential expression of human endogenous retroviral sequences similar to mouse mammary tumor virus in normal peripheral blood mononuclear cells. AIDS Res Hum Retroviruses 12, 833–840.[CrossRef]
    [Google Scholar]
  2. Andersson, M. L., Lindeskog, M., Medstrand, P., Westley, B., May, F. & Blomberg, J. ( 1999; ). Diversity of human endogenous retrovirus class II-like sequences. J Gen Virol 80, 255–260.
    [Google Scholar]
  3. Andersson, A. C., Yun, Z., Sperber, G. O., Larsson, E. & Blomberg, J. ( 2005; ). ERV3 and related sequences in humans: structure and RNA expression. J Virol 79, 9270–9284.[CrossRef]
    [Google Scholar]
  4. Bittner, J. J. ( 1936; ). Some possible effects of nursing on the mammary gland tumor incidence in mice. Science 84, 162
    [Google Scholar]
  5. Etkind, P., Du, J., Khan, A., Pillitteri, J. & Wiernik, P. H. ( 2000; ). Mouse mammary tumor virus-like ENV gene sequences in human breast tumors and in a lymphoma of a breast cancer patient. Clin Cancer Res 6, 1273–1278.
    [Google Scholar]
  6. Ford, C. E., Faedo, M. & Rawlinson, W. D. ( 2004a; ). Mouse mammary tumor virus-like RNA transcripts and DNA are found in affected cells of human breast cancer. Clin Cancer Res 10, 7284–7289.[CrossRef]
    [Google Scholar]
  7. Ford, C. E., Faedo, M., Crouch, R., Lawson, J. S. & Rawlinson, W. D. ( 2004b; ). Progression from normal breast pathology to breast cancer is associated with increasing prevalence of mouse mammary tumor virus-like sequences in men and women. Cancer Res 64, 4755–4759.[CrossRef]
    [Google Scholar]
  8. Holland, J. F. & Pogo, B. G. ( 2004; ). Mouse mammary tumor virus-like viral infection and human breast cancer. Clin Cancer Res 10, 5647–5649.[CrossRef]
    [Google Scholar]
  9. Liu, B., Wang, Y., Melana, S. M., Pelisson, I., Najfeld, V., Holland, J. F. & Pogo, B. G. ( 2001; ). Identification of a proviral structure in human breast cancer. Cancer Res 61, 1754–1759.
    [Google Scholar]
  10. Mant, C. & Cason, J. ( 2004; ). A human murine mammary tumour virus-like agent is an unconvincing aetiological agent for human breast cancer. Rev Med Virol 14, 169–177.[CrossRef]
    [Google Scholar]
  11. Medstrand, P. & Blomberg, J. ( 1993; ). Characterization of novel reverse transcriptase encoding human endogenous retroviral sequences similar to type A and type B retroviruses: differential transcription in normal human tissues. J Virol 67, 6778–6787.
    [Google Scholar]
  12. Medstrand, P., Lindeskog, M. & Blomberg, J. ( 1992; ). Expression of human endogenous retroviral sequences in peripheral blood mononuclear cells of healthy individuals. J Gen Virol 73, 2463–2466.[CrossRef]
    [Google Scholar]
  13. Pogo, B. G. & Holland, J. F. ( 1997; ). Possibilities of a viral etiology for human breast cancer. A review. Biol Trace Elem Res 56, 131–142.[CrossRef]
    [Google Scholar]
  14. Wang, Y., Holland, J. F., Bleiweiss, I. J., Melana, S., Liu, X., Pelisson, I., Cantarella, A., Stellrecht, K., Mani, S. & Pogo, B. G. ( 1995; ). Detection of mammary tumor virus env gene-like sequences in human breast cancer. Cancer Res 55, 5173–5179.
    [Google Scholar]
  15. Wärnberg, F., Nordgren, H., Bergkvist, L. & Holmberg, L. ( 2001; ). Tumour markers in breast carcinoma correlate with grade rather than with invasiveness. Br J Cancer 85, 869–874.[CrossRef]
    [Google Scholar]
  16. Xu, L., Sakalian, M., Shen, Z., Loss, G., Neuberger, J. & Mason, A. ( 2004; ). Cloning the human betaretrovirus proviral genome from patients with primary biliary cirrhosis. Hepatology 39, 151–156.[CrossRef]
    [Google Scholar]
  17. Yin, H., Medstrand, P., Andersson, M. L., Borg, A., Olsson, H. & Blomberg, J. ( 1997; ). Transcription of human endogenous retroviral sequences related to mouse mammary tumor virus in human breast and placenta: similar pattern in most malignant and nonmalignant breast tissues. AIDS Res Hum Retroviruses 13, 507–516.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.82767-0
Loading
/content/journal/jgv/10.1099/vir.0.82767-0
Loading

Data & Media loading...

Supplements

vol. , part 6, pp. 1806 – 1809

Synthetic control DNA templates of 140 bp [ PDF] (56 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error