1887

Abstract

Avian hepatitis E virus (HEV) was identified from chickens with hepatitis–splenomegaly syndrome. In this study, the complete genomic sequence of an apparently avirulent strain of avian HEV was determined to be 6649 nt in length, excluding the poly(A) tail, which is 5 nt shorter than the prototype avian HEV. Sequence analyses revealed that the ORF1 has 89.6 % nucleotide sequence identity, with numerous non-silent mutations and deletions, compared to the prototype strain. The ORF2 capsid gene showed 90.7 % sequence identity with six non-silent mutations, and ORF3 had four non-silent mutations with 97 % sequence identity. Overall, the apparently avirulent strain shares only 90.1 % nucleotide sequence identity with the prototype strain. The identification of significant non-silent mutations in the capsid gene and other regions suggests that these mutations may play a role in HEV attenuation. This is the first report of the full-length sequence of an apparently avirulent strain of HEV.

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2007-05-01
2024-12-06
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References

  1. Arankalle V. A., Chadha M. S., Tsarev S. A., Emerson S. U., Risbud A. R., Banerjee K., Purcell R. H. 1994; Seroepidemiology of water-borne hepatitis in India and evidence for a third enterically-transmitted hepatitis agent. Proc Natl Acad Sci U S A 91:3428–3432 [CrossRef]
    [Google Scholar]
  2. Billam P., Huang F. F., Sun Z. F., Pierson F. W., Duncan R. B., Elvinger F., Guenette D. K., Toth T. E., Meng X. J. 2005; Systematic pathogenesis and replication of avian hepatitis E virus in specific-pathogen-free adult chickens. J Virol 79:3429–3437 [CrossRef]
    [Google Scholar]
  3. Christodoulou C., Colbere-Garapin F., Macadam A., Taffs L. F., Marsden S., Minor P., Horaud F. 1990; Mapping of mutations associated with neurovirulence in monkeys infected with Sabin 1 poliovirus revertants selected at high temperature. J Virol 64:4922–4929
    [Google Scholar]
  4. Emerson S. U., Purcell R. H. 2003; Hepatitis E virus. Rev Med Virol 13:145–154 [CrossRef]
    [Google Scholar]
  5. Emerson S. U., Zhang M., Meng X. J., Nguyen H., St Claire M., Govindarajan S., Huang Y. K., Purcell R. H. 2001; Recombinant hepatitis E virus genomes infectious for primates: importance of capping and discovery of a cis-reactive element. Proc Natl Acad Sci U S A 98:15270–15275 [CrossRef]
    [Google Scholar]
  6. Emerson S. U., Anderson D., Arankalle A., Meng X. J., Purdy M., Schlauder G. G., Tsarev S. A. 2004; Hepevirus . In Virus Taxonomy. Eigth Report of the International Committee on Taxonomy of Viruses pp 853–857 Edited by Fauquet C. M., Mayo M. A., Maniloff J., Desselberger U., Ball L. A. London: Elsevier/Academic Press;
    [Google Scholar]
  7. Galán C., Enjuanes L., Almazán F. 2005; A point mutation within the replicase gene differentially affects coronavirus genome versus minigenome replication. J Virol 79:15016–15026 [CrossRef]
    [Google Scholar]
  8. Haqshenas G., Shivaprasad H. L., Woolcock P. R., Read D. H., Meng X. J. 2001; Genetic identification and characterization of a novel virus related to human hepatitis E virus from chickens with hepatitis-splenomegaly syndrome in the United States. J Gen Virol 82:2449–2462
    [Google Scholar]
  9. Harrison T. J. 1999; Hepatitis E virus – an update. Liver 19:171–176
    [Google Scholar]
  10. Hsieh S. Y., Meng X. J., Wu Y. H., Liu S. T., Tam A. W., Lin D. Y., Liaw Y. F. 1999; Identity of a novel swine hepatitis E virus in Taiwan forming a monophyletic group with Taiwan isolates of human hepatitis E virus. J Clin Microbiol 37:3828–3834
    [Google Scholar]
  11. Huang F. F., Haqshenas G., Guenette D. K., Halbur P. G., Schommer S. K., Pierson F. W., Toth T. E., Meng X. J. 2002a; Detection by reverse transcription-PCR and genetic characterization of field isolates of swine hepatitis E virus from pigs in different geographic regions of the United States. J Clin Microbiol 40:1326–1332 [CrossRef]
    [Google Scholar]
  12. Huang F. F., Haqshenas G., Shivaprasad H. L., Guenette D. K., Woolcock P. R., Larsen C. T., Pierson F. W., Elvinger F., Toth T. E., Meng X. J. 2002b; Heterogeneity and seroprevalence of a newly identified avian hepatitis e virus from chickens in the United States. J Clin Microbiol 40:4197–4202 [CrossRef]
    [Google Scholar]
  13. Huang F. F., Sun Z. F., Emerson S. U., Purcell R. H., Shivaprasad H. L., Pierson F. W., Toth T. E., Meng X. J. 2004; Determination and analysis of the complete genomic sequence of avian hepatitis E virus (avian HEV) and attempts to infect rhesus monkeys with avian HEV. J Gen Virol 85:1609–1618 [CrossRef]
    [Google Scholar]
  14. Khuroo M. S., Kamili S. 2003; Aetiology, clinical course and outcome of sporadic acute viral hepatitis in pregnancy. J Viral Hepat 10:61–69 [CrossRef]
    [Google Scholar]
  15. Matsuda H., Okada K., Takahashi K., Mishiro S. 2003; Severe hepatitis E virus infection after ingestion of uncooked liver from a wild boar. J Infect Dis 188:944 [CrossRef]
    [Google Scholar]
  16. Meng X. J. 2000; Novel strains of hepatitis E virus identified from humans and other animal species: is hepatitis E a zoonosis?. J Hepatol 33:842–845 [CrossRef]
    [Google Scholar]
  17. Meng X. J. 2003; Swine hepatitis E virus: cross-species infection and risk in xenotransplantation. Curr Top Microbiol Immunol 278:185–216
    [Google Scholar]
  18. Meng X. J. 2005; Hepatitis E as a zoonosis. In Viral Hepatitis , 3rd edn. pp 611–623 Edited by Thomas H., Zuckermann A., Lemon S. Oxford, UK: Blackwell Publishing Ltd;
    [Google Scholar]
  19. Meng X. J., Purcell R. H., Halbur P. G., Lehman J. R., Webb D. M., Tsareva T. S., Haynes J. S., Thacker B. J., Emerson S. U. 1997; A novel virus in swine is closely related to the human hepatitis E virus. Proc Natl Acad Sci U S A 94:9860–9865 [CrossRef]
    [Google Scholar]
  20. Nishizawa T., Takahashi M., Mizuo H., Miyajima H., Gotanda Y., Okamoto H. 2003; Characterization of Japanese swine and human hepatitis E virus isolates of genotype IV with 99 % identity over the entire genome. J Gen Virol 84:1245–1251 [CrossRef]
    [Google Scholar]
  21. Okamoto H., Takahashi M., Nishizawa T., Fukai K., Muramatsu U., Yoshikawa A. 2001; Analysis of the complete genome of indigenous swine hepatitis E virus isolated in Japan. Biochem Biophys Res Commun 289:929–936 [CrossRef]
    [Google Scholar]
  22. Payne C. J., Ellis T. M., Plant S. L., Gregory A. R., Wilcox G. E. 1999; Sequence data suggests big liver and spleen disease virus (BLSV) is genetically related to hepatitis E virus. Vet Microbiol 68:119–125 [CrossRef]
    [Google Scholar]
  23. Purcell R. H., Emerson S. U. 2001; Hepatitis E virus. In Fields Virology , 4th edn. pp 3051–3061 Edited by Knipe D. M., Howley P. M. Philadelphia, PA: Lippincott Williams & Wilkins;
    [Google Scholar]
  24. Sa-Carvalho D., Rieder E., Baxt B., Rodarte R., Tanuri A., Mason P. W. 1997; Tissue culture adaptation of foot-and-mouth disease virus selects viruses that bind to heparin and are attenuated in cattle. J Virol 71:5115–5123
    [Google Scholar]
  25. Schlauder G. G., Dawson G. J., Erker J. C., Kwo P. Y., Knigge M. F., Smalley D. L., Rosenblatt J. E., Desai S. M., Mushahwar I. K. 1998; The sequence and phylogenetic analysis of a novel hepatitis E virus isolated from a patient with acute hepatitis reported in the United States. J Gen Virol 79:447–456
    [Google Scholar]
  26. Sun Z. F., Larsen C. T., Dunlop A., Huang F. F., Pierson F. W., Toth T. E., Meng X. J. 2004; Genetic identification of avian hepatitis E virus (HEV) from healthy chicken flocks and characterization of the capsid gene of 14 avian HEV isolates from chickens with hepatitis-splenomegaly syndrome in different geographical regions of the United States. J Gen Virol 85:693–700 [CrossRef]
    [Google Scholar]
  27. Takahashi M., Nishizawa T., Miyajima H., Gotanda Y., Iita T., Tsuda F., Okamoto H. 2003a; Swine hepatitis E virus strains in Japan form four phylogenetic clusters comparable with those of Japanese isolates of human hepatitis E virus. J Gen Virol 84:851–862 [CrossRef]
    [Google Scholar]
  28. Takahashi M., Nishizawa T., Okamoto H. 2003b; Identification of a genotype III swine hepatitis E virus that was isolated from a Japanese pig born in 1990 and that is most closely related to Japanese isolates of human hepatitis E virus. J Clin Microbiol 41:1342–1343 [CrossRef]
    [Google Scholar]
  29. Tamada Y., Yano K., Yatsuhashi H., Inoue O., Mawatari F., Ishibashi H. 2004; Consumption of wild boar linked to cases of hepatitis E. J Hepatol 40:869–870 [CrossRef]
    [Google Scholar]
  30. Tatem J. M., Weeks-Levy C., Georgiu A., DiMichele S. J., Gorgacz E. J., Racaniello V. R., Cano F. R., Mento S. J. 1992; A mutation present in the amino terminus of Sabin 3 poliovirus VP1 protein is attenuating. J Virol 66:3194–3197
    [Google Scholar]
  31. Tei S., Kitajima N., Takahashi K., Mishiro S. 2003; Zoonotic transmission of hepatitis E virus from deer to human beings. Lancet 362:371–373 [CrossRef]
    [Google Scholar]
  32. van der Poel W. H., Verschoor F., van der Heide R., Herrera M. I., Vivo A., Kooreman M., de Roda Husman A. M. 2001; Hepatitis E virus sequences in swine related to sequences in humans, The Netherlands. Emerg Infect Dis 7:970–976 [CrossRef]
    [Google Scholar]
  33. Wang Y., Levine D. F., Bendall R. P., Teo C. G., Harrison T. J. 2001; Partial sequence analysis of indigenous hepatitis E virus isolated in the United Kingdom. J Med Virol 65:706–709 [CrossRef]
    [Google Scholar]
  34. Wang Y. C., Zhang H. Y., Xia N. S., Peng G., Lan H. Y., Zhuang H., Zhu Y. H., Li S. W., Tian K. G. other authors 2002; Prevalence, isolation, and partial sequence analysis of hepatitis E virus from domestic animals in China. J Med Virol 67:516–521 [CrossRef]
    [Google Scholar]
  35. Westrop G. D., Wareham K. A., Evans D. M., Dunn G., Minor P. D., Magrath D. I., Taffs F., Marsden S., Skinner M. A., Schild G. C. 1989; Genetic basis of attenuation of the Sabin type 3 oral poliovirus vaccine. J Virol 63:1338–1344
    [Google Scholar]
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