1887

Abstract

Penaeid shrimp infectious myonecrosis virus (IMNV) is a monosegmented double-stranded RNA virus that forms icosahedral virions and is tentatively assigned to the family . New examinations of the IMNV genome sequence revealed features not noted in the original report. These features include (i) two encoded ‘2A-like’ motifs, which are likely involved in open reading frame (ORF) 1 polyprotein ‘cleavage’; (ii) a 199 nt overlap between the end of ORF1 in frame 1 and the start of ORF2 in frame 3; and (iii) a ‘shifty heptamer’ motif and predicted RNA pseudoknot in the region of ORF1–ORF2 overlap, which probably allow ORF2 to be translated as a fusion with ORF1 by −1 ribosomal frameshifting. Features (ii) and (iii) bring the predicted ORF2 coding strategy of IMNV more in line with that of its closest phylogenetic relative, , as well as with that of several other members of the family including .

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2007-04-01
2024-04-16
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References

  1. Bekaert M., Rousset J. P. 2005; An extended signal involved in eukaryotic −1 frameshifting operates through modification of the E site tRNA. Mol Cell 17:61–68 [CrossRef]
    [Google Scholar]
  2. Bekaert M., Bidou L., Denise A., Duchateau-Nguyen G., Forest J. P., Froidevaux C., Hatin I., Rousset J. P., Termier M. 2003; Towards a computational model for −1 eukaryotic frameshifting sites. Bioinformatics 19:327–335 [CrossRef]
    [Google Scholar]
  3. Brierley I., Digard P., Inglis S. C. 1989; Characterization of an efficient coronavirus ribosomal frameshifting signal: requirement for an RNA pseudoknot. Cell 57:537–547 [CrossRef]
    [Google Scholar]
  4. Crawford L. J., Osman T. A., Booy F. P., Coutts R. H., Brasier C. M., Buck K. W. 2006; Molecular characterization of a partitivirus from Ophiostoma himal-ulmi . Virus Genes 33:33–39 [CrossRef]
    [Google Scholar]
  5. Dinman J. D., Icho T., Wickner R. B. 1991; A −1 ribosomal frameshift in a double-stranded RNA virus of yeast forms a gag–pol fusion protein. Proc Natl Acad Sci U S A 88:174–178 [CrossRef]
    [Google Scholar]
  6. Donnelly M. L., Hughes L. E., Luke G., Mendoza H., ten Dam E., Gani D., Ryan M. D. 2001; The ‘cleavage’ activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring ‘2A-like’sequences. J Gen Virol 82:1027–1041
    [Google Scholar]
  7. Giedroc D. P., Theimer C. A., Nixon P. L. 2000; Structure, stability and function of RNA pseudoknots involved in stimulating ribosomal frameshifting. J Mol Biol 298:167–185 [CrossRef]
    [Google Scholar]
  8. Huang C.-H., Lu C. L., Chiu H.-T. 2005; A heuristic approach for detecting RNA H-type pseudoknots. Bioinformatics 21:3501–3508 [CrossRef]
    [Google Scholar]
  9. Jacks T., Power M. D., Masiarz F. R., Luciw P. A., Barr P. J., Varmus H. E. 1988; Characterization of ribosomal frameshifting in HIV-1 gag–pol expression. Nature 331:280–283 [CrossRef]
    [Google Scholar]
  10. Li L., Wang A. L., Wang C. C. 2001; Structural analysis of the −1 ribosomal frameshift elements in giardiavirus mRNA. J Virol 75:10612–10622 [CrossRef]
    [Google Scholar]
  11. Lightner D. V., Pantoja C. R., Poulos B. T., Tang K. F. J., Redman R. M., Pasos de Andrade T., Bonami J. R. 2004; Infectious myonecrosis: new disease in Pacific white shrimp. Glob Aquac Advocate 7:85
    [Google Scholar]
  12. Naitow H., Tang J., Canady M., Wickner R. B., Johnson J. E. 2002; L-A virus at 3.4 Å resolution reveals particle architecture and mRNA decapping mechanism. Nat Struct Biol 9:725–728 [CrossRef]
    [Google Scholar]
  13. Palmenberg A. C., Parks G. D., Hall D. J., Ingraham R. H., Seng T. W., Pallai P. V. 1992; Proteolytic processing of the cardioviral P2 region: primary 2A/2B cleavage in clone-derived precursors. Virology 190:754–762 [CrossRef]
    [Google Scholar]
  14. Plant E. P., Jacobs K. L., Harger J. W., Meskauskas A., Jacobs J. L., Baxter J. L., Petrov A. N., Dinman J. D. 2003; The 9 Å solution: how mRNA pseudoknots promote efficient programmed −1 ribosomal frameshifting. RNA 9:168–174 [CrossRef]
    [Google Scholar]
  15. Poulos B. T., Tang K. F., Pantoja C. R., Bonami J. R., Lightner D. V. 2006; Purification and characterization of infectious myonecrosis virus of penaeid shrimp. J Gen Virol 87:987–996 [CrossRef]
    [Google Scholar]
  16. Reeder J., Giegerich R. 2004; Design, implementation and evaluation of a practical pseudoknot folding algorithm based on thermodynamics. BMC Bioinformatics 5:104 [CrossRef]
    [Google Scholar]
  17. Ribas J. C., Wickner R. B. 1998; The Gag domain of the Gag–Pol fusion protein directs incorporation into the L-A double-stranded RNA viral particles in Saccharomyces cerevisiae . J Biol Chem 273:9306–9311 [CrossRef]
    [Google Scholar]
  18. Wang A. L., Yang H.-M., Shen K. A., Wang C. C. 1993; Giardiavirus double-stranded RNA genome encodes a capsid polypeptide and a gag–pol-like fusion protein by a translational frameshift. Proc Natl Acad Sci U S A 90:8595–8599 [CrossRef]
    [Google Scholar]
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