1887

Abstract

The presence of BSE prion infectivity in asymptomatic cattle and its tissue distribution are important concerns for both human and veterinary health and food safety. In this work, a collection of tissues from asymptomatic cattle challenged orally with BSE and culled at 20, 24, 27, 30 and 33 months have been used to inoculate intracerebrally BoPrP-Tg110 mice expressing bovine PrP to assess their infectivity. Results demonstrate that BSE infectivity in asymptomatic cattle is essentially restricted to the nervous system, Peyer's patches and tonsils, as reported previously for terminally BSE-diseased cattle. BSE infectivity was detectable in Peyer's patches and tonsils at all time points analysed, but infectivity in nervous tissues (brainstem and sciatic nerve) was only detectable after 27 months from inoculation. Infectivity in brainstem increased markedly at 33 months after inoculation. All other investigated tissues or fluids (spleen, skeletal muscle, blood and urine) revealed no detectable infectivity throughout the time course studied.

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2007-04-01
2019-10-17
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References

  1. Andreoletti, O., Berthon, P., Marc, D., Sarradin, P., Grosclaude, J., van Keulen, L., Schelcher, F., Elsen, J. M. & Lantier, F. ( 2000; ). Early accumulation of PrP(Sc) in gut-associated lymphoid and nervous tissues of susceptible sheep from a Romanov flock with natural scrapie. J Gen Virol 81, 3115–3126.
    [Google Scholar]
  2. Andreoletti, O., Morel, N., Lacroux, C., Rouillon, V., Barc, C., Tabouret, G., Sarradin, P., Berthon, P., Bernardet, P. & other authors ( 2006; ). Bovine spongiform encephalopathy agent in spleen from an ARR/ARR orally exposed sheep. J Gen Virol 87, 1043–1046.[CrossRef]
    [Google Scholar]
  3. Aucouturier, P., Carp, R. I., Carnaud, C. & Wisniewski, T. ( 2000; ). Prion diseases and the immune system. Clin Immunol 96, 79–85.[CrossRef]
    [Google Scholar]
  4. Bons, N., Mestre-Frances, N., Belli, P., Cathala, F., Gajdusek, D. C. & Brown, P. ( 1999; ). Natural and experimental oral infection of nonhuman primates by bovine spongiform encephalopathy agents. Proc Natl Acad Sci U S A 96, 4046–4051.[CrossRef]
    [Google Scholar]
  5. Borchelt, D. R., Davis, J., Fischer, M., Lee, M. K., Slunt, H. H., Ratovitsky, T., Regard, J., Copeland, N. G., Jenkins, N. A. & other authors ( 1996; ). A vector for expressing foreign genes in the brains and hearts of transgenic mice. Genet Anal 13, 159–163.[CrossRef]
    [Google Scholar]
  6. Bosque, P. J., Ryou, C., Telling, G., Peretz, D., Legname, G., DeArmond, S. J. & Prusiner, S. B. ( 2002; ). Prions in skeletal muscle. Proc Natl Acad Sci U S A 99, 3812–3817.[CrossRef]
    [Google Scholar]
  7. Bruce, M. E., Will, R. G., Ironside, J. W., McConnell, I., Drummond, D., Suttie, A., McCardle, L., Chree, A., Hope, J. & other authors ( 1997; ). Transmissions to mice indicate that ‘new variant' CJD is caused by the BSE agent. Nature 389, 498–501.[CrossRef]
    [Google Scholar]
  8. Brun, A., Castilla, J., Ramirez, M. A., Prager, K., Parra, B., Salguero, F. J., Shiveral, D., Sanchez, C., Sanchez-Vizcaino, J. M. & other authors ( 2004; ). Proteinase K enhanced immunoreactivity of the prion protein-specific monoclonal antibody 2A11. Neurosci Res 48, 75–83.[CrossRef]
    [Google Scholar]
  9. Buschmann, A. & Groschup, M. H. ( 2005; ). Highly bovine spongiform encephalopathy-sensitive transgenic mice confirm the essential restriction of infectivity to the nervous system in clinically diseased cattle. J Infect Dis 192, 934–942.[CrossRef]
    [Google Scholar]
  10. Castilla, J., Gutierrez Adan, A., Brun, A., Pintado, B., Ramirez, M. A., Parra, B., Doyle, D., Rogers, M., Salguero, F. J. & other authors ( 2003; ). Early detection of PrP(res) in BSE-infected bovine PrP transgenic mice. Arch Virol 148, 677–691.[CrossRef]
    [Google Scholar]
  11. Castilla, J., Gutierrez-Adan, A., Brun, A., Pintado, B., Parra, B., Ramirez, M. A., Salguero, F. J., Diaz San Segundo, F., Rabano, A. & other authors ( 2004; ). Different behavior toward bovine spongiform encephalopathy infection of bovine prion protein transgenic mice with one extra repeat octapeptide insert mutation. J Neurosci 24, 2156–2164.[CrossRef]
    [Google Scholar]
  12. Feraudet, C., Morel, N., Simon, S., Volland, H., Frobert, Y., Creminon, C., Vilette, D., Lehmann, S. & Grassi, J. ( 2005; ). Screening of 145 anti-PrP monoclonal antibodies for their capacity to inhibit PrPSc replication in infected cells. J Biol Chem 280, 11247–11258.[CrossRef]
    [Google Scholar]
  13. Grassi, J., Comoy, E., Simon, S., Creminon, C., Frobert, Y., Trapmann, S., Schimmel, H., Hawkins, S. A., Moynagh, J. & other authors ( 2001; ). Rapid test for the preclinical postmortem diagnosis of BSE in central nervous system tissue. Vet Rec 149, 577–582.[CrossRef]
    [Google Scholar]
  14. Hathaway, L. J. & Kraehenbuhl, J. P. ( 2000; ). The role of M cells in mucosal immunity. Cell Mol Life Sci 57, 323–332.[CrossRef]
    [Google Scholar]
  15. Heggebo, R., Press, C. M., Gunnes, G., Ulvund, M. J., Tranulis, M. A. & Lsverk, T. ( 2003; ). Detection of PrPSc in lymphoid tissues of lambs experimentally exposed to the scrapie agent. J Comp Pathol 128, 172–181.[CrossRef]
    [Google Scholar]
  16. Heppner, F. L., Christ, A. D., Klein, M. A., Prinz, M., Fried, M., Kraehenbuhl, J. P. & Aguzzi, A. ( 2001; ). Transepithelial prion transport by M cells. Nat Med 7, 976–977.[CrossRef]
    [Google Scholar]
  17. Herzog, C., Sales, N., Etchegaray, N., Charbonnier, A., Freire, S., Dormont, D., Deslys, J. P. & Lasmezas, C. I. ( 2004; ). Tissue distribution of bovine spongiform encephalopathy agent in primates after intravenous or oral infection. Lancet 363, 422–428.[CrossRef]
    [Google Scholar]
  18. Hill, A. F., Desbruslais, M., Joiner, S., Sidle, K. C., Gowland, I., Collinge, J., Doey, L. J. & Lantos, P. ( 1997; ). The same prion strain causes vCJD and BSE. Nature 389, 448–450, 526.[CrossRef]
    [Google Scholar]
  19. Jeffrey, M., Ryder, S., Martin, S., Hawkins, S. A., Terry, L., Berthelin-Baker, C. & Bellworthy, S. J. ( 2001; ). Oral inoculation of sheep with the agent of bovine spongiform encephalopathy (BSE). I. Onset and distribution of disease-specific PrP accumulation in brain and viscera. J Comp Pathol 124, 280–289.[CrossRef]
    [Google Scholar]
  20. Lasmezas, C. I., Comoy, E., Hawkins, S., Herzog, C., Mouthon, F., Konold, T., Auvre, F., Correia, E., Lescoutra-Etchegaray, N. & other authors ( 2005; ). Risk of oral infection with bovine spongiform encephalopathy agent in primates. Lancet 365, 781–783.[CrossRef]
    [Google Scholar]
  21. Madec, J. Y., Groschup, M. H., Calavas, D., Junghans, F. & Baron, T. ( 2000; ). Protease-resistant prion protein in brain and lymphoid organs of sheep within a naturally scrapie-infected flock. Microb Pathog 28, 353–362.[CrossRef]
    [Google Scholar]
  22. Prusiner, S. B. ( 2004; ). Early evidence that a protease-resistant protein is an active component of the infectious prion. Cell 116, S109 [CrossRef]
    [Google Scholar]
  23. Race, R., Jenny, A. & Sutton, D. ( 1998; ). Scrapie infectivity and proteinase K-resistant prion protein in sheep placenta, brain, spleen, and lymph node: implications for transmission and antemortem diagnosis. J Infect Dis 178, 949–953.[CrossRef]
    [Google Scholar]
  24. Scott, M., Foster, D., Mirenda, C., Serban, D., Coufal, F., Walchli, M., Torchia, M., Groth, D., Carlson, G. & other authors ( 1989; ). Transgenic mice expressing hamster prion protein produce species-specific scrapie infectivity and amyloid plaques. Cell 59, 847–857.[CrossRef]
    [Google Scholar]
  25. Scott, M., Groth, D., Foster, D., Torchia, M., Yang, S. L., DeArmond, S. J. & Prusiner, S. B. ( 1993; ). Propagation of prions with artificial properties in transgenic mice expressing chimeric PrP genes. Cell 73, 979–988.[CrossRef]
    [Google Scholar]
  26. Thomzig, A., Kratzel, C., Lenz, G., Kruger, D. & Beekes, M. ( 2003; ). Widespread PrP(Sc) accumulation in muscles of hamsters orally infected with scrapie. EMBO Rep 4, 530–533.[CrossRef]
    [Google Scholar]
  27. Thomzig, A., Schulz-Schaeffer, W., Kratzel, C., Mai, J. & Beekes, M. ( 2004; ). Preclinical deposition of pathological prion protein PrPSc in muscles of hamsters orally exposed to scrapie. J Clin Invest 113, 1465–1472.[CrossRef]
    [Google Scholar]
  28. van Keulen, L. J., Schreuder, B. E., Meloen, R. H., Mooij-Harkes, G., Vromans, M. E. & Langeveld, J. P. ( 1996; ). Immunohistochemical detection of prion protein in lymphoid tissues of sheep with natural scrapie. J Clin Microbiol 34, 1228–1231.
    [Google Scholar]
  29. Wells, G. A., Dawson, M., Hawkins, S. A., Green, R. B., Dexter, I., Francis, M. E., Simmons, M. M., Austin, A. R. & Horigan, M. W. ( 1994; ). Infectivity in the ileum of cattle challenged orally with bovine spongiform encephalopathy. Vet Rec 135, 40–41.[CrossRef]
    [Google Scholar]
  30. Wells, G. A., Hawkins, S. A., Green, R. B., Austin, A. R., Dexter, I., Spencer, Y. I., Chaplin, M. J., Stack, M. J. & Dawson, M. ( 1998; ). Preliminary observations on the pathogenesis of experimental bovine spongiform encephalopathy (BSE): an update. Vet Rec 142, 103–106.[CrossRef]
    [Google Scholar]
  31. Wells, G. A., Spiropoulos, J., Hawkins, S. A. & Ryder, S. J. ( 2005; ). Pathogenesis of experimental bovine spongiform encephalopathy: preclinical infectivity in tonsil and observations on the distribution of lingual tonsil in slaughtered cattle. Vet Rec 156, 401–407.[CrossRef]
    [Google Scholar]
  32. Wilesmith, J. W., Wells, G. A., Cranwell, M. P. & Ryan, J. B. ( 1988; ). Bovine spongiform encephalopathy: epidemiological studies. Vet Rec 123, 638–644.
    [Google Scholar]
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