@article{mbs:/content/journal/jgv/10.1099/vir.0.82568-0, author = "Brun, Alejandro and Gutiérrez-Adán, Alfonso and Castilla, Joaquín and Pintado, Belén and Díaz-San Segundo, Fayna and Cano, María J. and Alamillo, Elia and Espinosa, Juan C. and Torres, Juan M.", title = "Reduced susceptibility to bovine spongiform encephalopathy prions in transgenic mice expressing a bovine PrP with five octapeptide repeats", journal= "Journal of General Virology", year = "2007", volume = "88", number = "6", pages = "1842-1849", doi = "https://doi.org/10.1099/vir.0.82568-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.82568-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "In this work, transgenic (Tg) mice were generated expressing a bovine prion protein containing five octarepeats (BoPrP5OR-Tg). After intracerebral inoculation of bovine spongiform encephalopathy (BSE) inoculum, these mice suffered a BSE-like neuropathology but survived longer compared with homologous Tg mice expressing similar levels of a six octarepeat BoPrP protein (BoPrP6OR-Tg). De novo-generated five octarepeat (5OR) PrPSc showed no biochemical differences from 6OR-PrPSc, and the proteinase K-resistant core (PrPres) was biochemically indistinguishable from the 6OR counterpart. Lower susceptibility to BSE is suggested for BoPrP5OR-Tg mice, as they were not as efficient at replicating BSE prions from the same natural source inoculum as BoPrP6OR-Tg mice expressing similar PrPC levels. These results raise the possibility of selecting cattle breeds bearing the 5OR Prnp allele that are less susceptible to prion infection.", }