1887

Abstract

In this work, transgenic (Tg) mice were generated expressing a bovine prion protein containing five octarepeats (BoPrP-Tg). After intracerebral inoculation of bovine spongiform encephalopathy (BSE) inoculum, these mice suffered a BSE-like neuropathology but survived longer compared with homologous Tg mice expressing similar levels of a six octarepeat BoPrP protein (BoPrP-Tg). -generated five octarepeat (5OR) PrP showed no biochemical differences from 6OR-PrP, and the proteinase K-resistant core (PrP) was biochemically indistinguishable from the 6OR counterpart. Lower susceptibility to BSE is suggested for BoPrP-Tg mice, as they were not as efficient at replicating BSE prions from the same natural source inoculum as BoPrP-Tg mice expressing similar PrP levels. These results raise the possibility of selecting cattle breeds bearing the 5OR allele that are less susceptible to prion infection.

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2007-06-01
2019-10-19
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