1887

Abstract

Four monoclonal antibodies (mAbs) recognizing distinct antigenic sites on the HA2 glycopolypeptide of influenza virus A/Dunedin/4/73 (H3N2) have been tested for protection. When applied intravenously before infection, three of them increased the survival of BALB/c mice infected with 1 LD homologous virus. The protection resulted simultaneously in 2 days earlier clearance of virus from the lungs. These three antibodies inhibited the fusion activity of virus in previous experiments. One of them, specific to N-terminal aa 1–38 of the HA2 glycopolypeptide, was also tested for protection against the heterologous virus A/Mississippi/1/85 (H3N2). Protection similar to that against the homologous virus was observed. The fourth mAb, without fusion-inhibition activity, did not protect mice. It is concluded that antibodies specific to the antigenically conserved HA2 glycopolypeptide that exhibit fusion-inhibition activity can contribute to the protection of infected mice and mediate more effective recovery from infection.

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2007-03-01
2021-10-24
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References

  1. Becht H., Huang R. T. C., Fleischer B., Boschek C. B., Rott R. 1984; Immunogenic properties of the small chain HA2 of the haemagglutinin of influenza viruses. J Gen Virol 65:173–183 [CrossRef]
    [Google Scholar]
  2. Bystrická M., Petríková M., Zat'ovičová M., Soláriková L., Kostolanský F., Mucha V., Russ G. 1997; Monoclonal antibodies to the distinct antigenic sites on glycoproteins C and B and their protective abilities in herpes simplex virus infection. Acta Virol 41:5–12
    [Google Scholar]
  3. Cox N. J., Tamblyn S. E., Tam T. 2003; Influenza pandemic planning. Vaccine 21:1801–1803 [CrossRef]
    [Google Scholar]
  4. Ey P. L., Prowse S. J., Jenkin C. R. 1978; Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using Protein A-Sepharose. Immunochemistry 15:429–436 [CrossRef]
    [Google Scholar]
  5. Fislová T., Sládková T., Gocník M., Mucha V., Varečková E., Kostolanský F. 2005; Differences in antibody responses of mice to intranasal or intraperitoneal immunization with influenza A virus and vaccination with subunit influenza vaccine. Acta Virol 49:243–250
    [Google Scholar]
  6. Gerhard W. 2001; The role of the antibody response in influenza virus infection. Curr Top Microbiol Immunol 260:171–190
    [Google Scholar]
  7. Gould K. G., Scotney H., Townsend A. R., Bastin J., Brownlee G. G. 1987; Mouse H-2k-restricted cytotoxic T cells recognize antigenic determinants in both the HA1 and HA2 subunits of the influenza A/PR/8/34 hemagglutinin. J Exp Med 166:693–701 [CrossRef]
    [Google Scholar]
  8. Jackson D. C., Drummer H. E., Brown L. E. 1994; Conserved determinants for CD4+ T cells within the light chain of the H3 hemagglutinin molecule of influenza virus. Virology 198:613–623 [CrossRef]
    [Google Scholar]
  9. Katz J. M., Brown L. E., Ffrench R. A., White D. O. 1985; Murine helper T lymphocyte response to influenza virus: recognition of haemagglutinin by subtype-specific and cross-reactive T cell clones. Vaccine 3:257–262 [CrossRef]
    [Google Scholar]
  10. Knossow M., Gaudier M., Douglas A., Barrere B., Bizebard T., Barbey C., Gigant B., Skehel J. J. 2002; Mechanism of neutralization of influenza virus infectivity by antibodies. Virology 302:294–298 [CrossRef]
    [Google Scholar]
  11. Kostolanský F., Mucha V., Slováková R., Varečková E. 2002; Natural influenza A virus infection of mice elicits strong antibody response to HA2 glycopolypeptide. Acta Virol 46:229–236
    [Google Scholar]
  12. Kuwano K., Scott M., Young J. F., Ennis F. A. 1988; HA2 subunit of influenza A H1 and H2 subtype induces a protective cross-reactive cytotoxic T lymphocyte response. J Immunol 140:1264–1268
    [Google Scholar]
  13. Mbawuike I. N., Dillion S. B., Demuth S. G., Jones C. S., Cate T. R., Couch R. B. 1994; Influenza A subtype cross-protection after immunization of outbred mice with a purified chimeric NS1/HA2 influenza virus protein. Vaccine 12:1340–1348 [CrossRef]
    [Google Scholar]
  14. Mitchell J. A., Green T. D., Bright R. A., Ross T. M. 2003; Induction of heterosubtypic immunity to influenza A virus using a DNA vaccine expressing hemagglutinin-C3d fusion proteins. Vaccine 21:902–914 [CrossRef]
    [Google Scholar]
  15. Mozdzanowska K., Feng J., Eid M., Kragol G., Cudic M., Otvos L. Jr, Gerhard W. 2003; Induction of influenza type A virus-specific resistance by immunization of mice with a synthetic multiple antigenic peptide vaccine that contains ectodomains of matrix protein 2. Vaccine 21:2616–2626 [CrossRef]
    [Google Scholar]
  16. Nobusawa E., Aoyama T., Kato H., Suzuki Y., Tateno Y., Nakajima K. 1991; Comparison of complete amino acid sequences and receptor-binding properties among 13 serotypes of hemagglutinins of influenza A viruses. Virology 182:475–485 [CrossRef]
    [Google Scholar]
  17. Okuno Y., Isegawa Y., Sasao F., Ueda S. 1993; A common neutralizing epitope conserved between the hemagglutinins of influenza A virus H1 and H2 strains. J Virol 67:2552–2558
    [Google Scholar]
  18. Okuno Y., Matsumoto K., Isegawa Y., Ueda S. 1994; Protection against the mouse-adapted A/FM/1/47 strain of influenza A virus in mice by a monoclonal antibody with cross-neutralizing activity among H1 and H2 strains. J Virol 68:517–520
    [Google Scholar]
  19. Russ G., Poláková K., Kostolanský F., Styk B., Vančíková M. 1987; Monoclonal antibodies to glycopeptides HA1 and HA2 of influenza virus hemagglutinin. Acta Virol 31:374–386
    [Google Scholar]
  20. Saikh K. U., Martin J. D., Nishikawa A. H., Dillon S. B. 1995; Influenza A virus-specific H2d restricted cross-reactive cytotoxic T lymphocyte epitope(s) detected in the hemagglutinin HA2 subunit of A/Udorn/72. Virology 214:445–452 [CrossRef]
    [Google Scholar]
  21. Sánchez-Fauquier A., Villanueva N., Melero J. A. 1987; Isolation of cross-reactive, subtype-specific monoclonal antibodies against influenza virus HA1 and HA2 hemagglutinin subunits. Arch Virol 97:251–265 [CrossRef]
    [Google Scholar]
  22. Sánchez-Fauquier A., Guillen M., Martin J., Kendal A. P., Melero J. A. 1991; Conservation of epitopes recognized by monoclonal antibodies against the separated subunits of influenza hemagglutinin among type A viruses of the same and different subtypes. Arch Virol 116:285–292 [CrossRef]
    [Google Scholar]
  23. Skehel J. J., Wiley D. C. 2000; Receptor binding and membrane fusion in virus entry: the influenza hemagglutinin. Annu Rev Biochem 69:531–569 [CrossRef]
    [Google Scholar]
  24. Smirnov Y. A., Lipatov A. S., Gitelman A. K., Claas E. C., Osterhaus A. D. 2000; Prevention and treatment of bronchopneumonia in mice caused by mouse-adapted variant of avian H5N2 influenza A virus using monoclonal antibody against conserved epitope in the HA stem. Arch Virol 145:1733–1741 [CrossRef]
    [Google Scholar]
  25. Spier R. E. 2005; On the need for, and the delivery of, cross-protective vaccines. Vaccine 23:2027–2029 [CrossRef]
    [Google Scholar]
  26. Styk B., Russ G., Poláková K. 1979; Antigenic glycopolypeptides HA1 and HA2 of influenza virus haemagglutinin. III. Reactivity with human convalescent sera. Acta Virol 23:1–8
    [Google Scholar]
  27. Tamura M., Webster R. G., Ennis F. A. 1994; Subtype cross-reactive, infection-enhancing antibody responses to influenza A viruses. J Virol 68:3499–3504
    [Google Scholar]
  28. Tamura S., Tanimoto T., Kurat T. 2005; Mechanism of broad cross-protection provided by influenza virus infection and their application to vaccines. Jpn J Infect Dis 58:195–207
    [Google Scholar]
  29. Tkáčová M., Varečková E., Baker I. C., Love J. M., Ziegler T. 1997; Evaluation of monoclonal antibodies for subtyping of currently circulating human type A influenza viruses. J Clin Microbiol 35:1196–1198
    [Google Scholar]
  30. Varečková E., Cox N., Klimov A. 2002; Evaluation of the subtype specificity of monoclonal antibodies raised against H1 and H3 subtypes of human influenza A virus hemagglutinins. J Clin Microbiol 40:2220–2223 [CrossRef]
    [Google Scholar]
  31. Varečková E., Mucha V., Wharton S. A., Kostolanský F. 2003a; Inhibition of fusion activity of influenza A haemagglutinin mediated by HA2-specific monoclonal antibodies. Arch Virol 148:469–486 [CrossRef]
    [Google Scholar]
  32. Varečková E., Wharton S. A., Mucha V., Gocník M., Kostolanský F. 2003b; A monoclonal antibody specific to the HA2 glycoprotein of influenza A virus hemagglutinin that inhibits its fusion activity reduces replication of the virus. Acta Virol 47:229–236
    [Google Scholar]
  33. Varečková E., Blaškovičová H., Gocník M., Mikas J., Adamčáková J., Fislová T., Kostolanský F., Mucha V. 2006; Evaluation of clinical specimens for influenza A virus positivity using various diagnostic methods. Acta Virol 50:181–186
    [Google Scholar]
  34. Wabuke-Bunoti M. A., Taku A., Fan D. P., Kent S., Webster R. G. 1984; Cytolytic T lymphocyte and antibody responses to synthetic peptides of influenza virus hemagglutinin. J Immunol 133:2194–2201
    [Google Scholar]
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