1887

Abstract

(BVDV) is an economically important pathogen of cattle that is maintained in the population by persistently infected animals. Virus infection may result in reproductive failure, respiratory disease and diarrhoea in naïve, susceptible bovines. Here, the construction and characterization of a novel vectored vaccine, which is based on the incorporation of genes encoding BVDV structural proteins (C, E, E1, E2) into a bacterial artificial chromosome of the equine herpesvirus type 1 (EHV-1) vaccine strain RacH, are reported. The reconstituted vectored virus, rH_BVDV, expressed BVDV structural proteins efficiently and was indistinguishable from parental vector virus with respect to growth properties in cultured cells. Intramuscular immunization of seronegative cattle with rH_BVDV resulted in induction of BVDV-specific serum neutralizing and ELISA antibodies. Upon experimental challenge infection of immunized calves with the heterologous BVDV strain Ib SE5508, a strong anamnestic boost of the neutralizing-antibody response was observed in all vaccinated animals. Immunized animals presented with reduced viraemia levels and decreased nasal virus shedding, and maintained higher leukocyte counts than mock-vaccinated controls.

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2007-03-01
2019-11-13
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References

  1. Beer, M., Hehnen, H. R., Wolfmeyer, A., Poll, G., Kaaden, O. R. & Wolf, G. ( 2000; ). A new inactivated BVDV genotype I and II vaccine. An immunisation and challenge study with BVDV genotype I. Vet Microbiol 77, 195–208.[CrossRef]
    [Google Scholar]
  2. Bolin, S. R. & Ridpath, J. F. ( 1996; ). Glycoprotein E2 of bovine viral diarrhea virus expressed in insect cells provides calves limited protection from systemic infection and disease. Arch Virol 141, 1463–1477.[CrossRef]
    [Google Scholar]
  3. Borst, E. M., Hahn, G., Koszinowski, U. H. & Messerle, M. ( 1999; ). Cloning of the human cytomegalovirus (HCMV) genome as an infectious bacterial artificial chromosome in Escherichia coli: a new approach for construction of HCMV mutants. J Virol 73, 8320–8329.
    [Google Scholar]
  4. Borst, E. M., Crnkovic-Mertens, I. & Messerle, M. ( 2004; ). Cloning of beta-herpesvirus genomes as bacterial artificial chromosomes. Methods Mol Biol 256, 221–239.
    [Google Scholar]
  5. Brune, W., Menard, C., Hobom, U., Odenbreit, S., Messerle, M. & Koszinowski, U. H. ( 1999; ). Rapid identification of essential and nonessential herpesvirus genes by direct transposon mutagenesis. Nat Biotechnol 17, 360–364.[CrossRef]
    [Google Scholar]
  6. Brune, W., Messerle, M. & Koszinowski, U. H. ( 2000; ). Forward with BACs: new tools for herpesvirus genomics. Trends Genet 16, 254–259.[CrossRef]
    [Google Scholar]
  7. Bruschke, C. J., van Oirschot, J. T. & van Rijn, P. A. ( 1999; ). An experimental multivalent bovine virus diarrhea virus E2 subunit vaccine and two experimental conventionally inactivated vaccines induce partial fetal protection in sheep. Vaccine 17, 1983–1991.[CrossRef]
    [Google Scholar]
  8. Campbell, J. R. ( 2004; ). Effect of bovine viral diarrhea virus in the feedlot. Vet Clin North Am Food Anim Pract 20, 39–50.[CrossRef]
    [Google Scholar]
  9. Carlsson, U., Alenius, S. & Sundquist, B. ( 1991; ). Protective effect of an ISCOM bovine virus diarrhoea virus (BVDV) vaccine against an experimental BVDV infection in vaccinated and non-vaccinated pregnant ewes. Vaccine 9, 577–580.[CrossRef]
    [Google Scholar]
  10. Chowdhury, S. I., Ludwig, H. & Buhk, H. J. ( 1988; ). Molecular biological characterization of equine herpesvirus type 1 (EHV-1) isolates from ruminant hosts. Virus Res 11, 127–139.[CrossRef]
    [Google Scholar]
  11. Collett, M. S., Anderson, D. K. & Retzel, E. ( 1988a; ). Comparisons of the pestivirus bovine viral diarrhoea virus with members of the flaviviridae. J Gen Virol 69, 2637–2643.[CrossRef]
    [Google Scholar]
  12. Collett, M. S., Larson, R., Belzer, S. K. & Retzel, E. ( 1988b; ). Proteins encoded by bovine viral diarrhea virus: the genomic organization of a pestivirus. Virology 165, 200–208.[CrossRef]
    [Google Scholar]
  13. Collett, M. S., Wiskerchen, M., Welniak, E. & Belzer, S. K. ( 1991; ). Bovine viral diarrhea virus genomic organization. Arch Virol 3, 19–27.
    [Google Scholar]
  14. Corapi, W. V., Donis, R. O. & Dubovi, E. J. ( 1990a; ). Characterization of a panel of monoclonal antibodies and their use in the study of the antigenic diversity of bovine viral diarrhea virus. Am J Vet Res 51, 1388–1394.
    [Google Scholar]
  15. Corapi, W. V., Elliott, R. D., French, T. W., Arthur, D. G., Bezek, D. M. & Dubovi, E. J. ( 1990b; ). Thrombocytopenia and hemorrhages in veal calves infected with bovine viral diarrhea virus. J Am Vet Med Assoc 196, 590–596.
    [Google Scholar]
  16. DesCoteaux, L., Cecyre, D., Elsener, J. & Beauchamp, G. ( 2003; ). Comparison of humoral immune responses in dairy heifers vaccinated with 3 different commercial vaccines against bovine viral diarrhea virus and bovine herpesvirus-1. Can Vet J 44, 816–821.
    [Google Scholar]
  17. Dubovi, E. J. ( 1994; ). Impact of bovine viral diarrhea virus on reproductive performance in cattle. Vet Clin North Am Food Anim Pract 10, 503–514.
    [Google Scholar]
  18. Dudek, T. & Knipe, D. M. ( 2006; ). Replication-defective viruses as vaccines and vaccine vectors. Virology 344, 230–239.[CrossRef]
    [Google Scholar]
  19. Ellis, J. A., Davis, W. C., Belden, E. L. & Pratt, D. L. ( 1988; ). Flow cytofluorimetric analysis of lymphocyte subset alterations in cattle infected with bovine viral diarrhea virus. Vet Pathol 25, 231–236.[CrossRef]
    [Google Scholar]
  20. Ellis, J., West, K., Cortese, V., Konoby, C. & Weigel, D. ( 2001; ). Effect of maternal antibodies on induction and persistence of vaccine-induced immune responses against bovine viral diarrhea virus type II in young calves. J Am Vet Med Assoc 219, 351–356.[CrossRef]
    [Google Scholar]
  21. Fulton, R. W., Briggs, R. E., Ridpath, J. F., Saliki, J. T., Confer, A. W., Payton, M. E., Duff, G. C., Step, D. L. & Walker, D. A. ( 2005; ). Transmission of bovine viral diarrhea virus 1b to susceptible and vaccinated calves by exposure to persistently infected calves. Can J Vet Res 69, 161–169.
    [Google Scholar]
  22. Grooms, D. L., Brock, K. V. & Ward, L. A. ( 1998; ). Detection of cytopathic bovine viral diarrhea virus in the ovaries of cattle following immunization with a modified live bovine viral diarrhea virus vaccine. J Vet Diagn Invest 10, 130–134.[CrossRef]
    [Google Scholar]
  23. Hamers, C., Couvreur, B., Dehan, P., Letellier, C., Fischer, L., Brun, A. J., Lewalle, P., Michaux, C., Pastoret, P. P. & Kerkhofs, P. ( 2003; ). Assessment of the clinical and virological protection provided by a commercial inactivated bovine viral diarrhoea virus genotype 1 vaccine against a BVDV genotype 2 challenge. Vet Rec 153, 236–240.[CrossRef]
    [Google Scholar]
  24. Heister, T. G., Vogtlin, A., Muller, L., Heid, I. & Fraefel, C. ( 2004; ). Construction of HSV-1 BACs and their use for packaging of HSV-1-based amplicon vectors. Methods Mol Biol 256, 241–256.
    [Google Scholar]
  25. Hendrie, P. C. & Russell, D. W. ( 2005; ). Gene targeting with viral vectors. Mol Ther 12, 9–17.[CrossRef]
    [Google Scholar]
  26. Howard, C. J. ( 1990; ). Immunological responses to bovine virus diarrhoea virus infections. Rev Sci Tech 9, 95–103.
    [Google Scholar]
  27. Howard, C. J., Clarke, M. C. & Brownlie, J. ( 1989; ). Protection against respiratory infection with bovine virus diarrhoea virus by passively acquired antibody. Vet Microbiol 19, 195–203.[CrossRef]
    [Google Scholar]
  28. Hubert, P. H., Birkenmaier, S., Rziha, H. J. & Osterrieder, N. ( 1996; ). Alterations in the equine herpesvirus type-1 (EHV-1) strain RacH during attenuation. Zentralbl Veterinarmed B 43, 1–14.[CrossRef]
    [Google Scholar]
  29. Kawaguchi, Y. & Tanaka, M. ( 2004; ). BAC system: a novel method for manipulation of herpesvirus genomes based on bacterial genetics. Uirusu 54, 255–264.[CrossRef]
    [Google Scholar]
  30. Keller, S. L., Jefferson, B. J., Jacobs, R. M. & Wood, R. D. ( 2006; ). Effects of noncytopathic type 2 bovine viral diarrhea virus on the proliferation of bone marrow progenitor cells. Can J Vet Res 70, 20–27.
    [Google Scholar]
  31. Kelling, C. L., Hunsaker, B. D., Steffen, D. J., Topliff, C. L., Abdelmagid, O. Y. & Eskridge, K. M. ( 2005; ). Characterization of protection from systemic infection and disease by use of a modified-live noncytopathic bovine viral diarrhea virus type 1 vaccine in experimentally infected calves. Am J Vet Res 66, 1785–1791.[CrossRef]
    [Google Scholar]
  32. Kozasa, T., Tajima, M., Yasutomi, I., Sano, K., Ohashi, K. & Onuma, M. ( 2005; ). Relationship of bovine viral diarrhea virus persistent infection to incidence of diseases on dairy farms based on bulk tank milk test by RT-PCR. Vet Microbiol 106, 41–47.[CrossRef]
    [Google Scholar]
  33. Kyhse-Andersen, J. ( 1984; ). Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. J Biochem Biophys Methods 10, 203–209.[CrossRef]
    [Google Scholar]
  34. Lindberg, A. L. ( 2003; ). Bovine viral diarrhoea virus infections and its control. A review. Vet Q 25, 1–16.[CrossRef]
    [Google Scholar]
  35. Loneragan, G. H., Thomson, D. U., Montgomery, D. L., Mason, G. L. & Larson, R. L. ( 2005; ). Prevalence, outcome, and health consequences associated with persistent infection with bovine viral diarrhea virus in feedlot cattle. J Am Vet Med Assoc 226, 595–601.[CrossRef]
    [Google Scholar]
  36. McClurkin, A. W., Littledike, E. T., Cutlip, R. C., Frank, G. H., Coria, M. F. & Bolin, S. R. ( 1984; ). Production of cattle immunotolerant to bovine viral diarrhea virus. Can J Comp Med 48, 156–161.
    [Google Scholar]
  37. McGregor, A. & Schleiss, M. R. ( 2001; ). Recent advances in herpesvirus genetics using bacterial artificial chromosomes. Mol Genet Metab 72, 8–14.[CrossRef]
    [Google Scholar]
  38. Messerle, M., Crnkovic, I., Hammerschmidt, W., Ziegler, H. & Koszinowski, U. H. ( 1997; ). Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome. Proc Natl Acad Sci U S A 94, 14759–14763.[CrossRef]
    [Google Scholar]
  39. Miletic, H., Bruns, M., Tsiakas, K., Vogt, B., Rezai, R., Baum, C., Kuhlke, K., Cosset, F. L., Ostertag, W. & other authors ( 1999; ). Retroviral vectors pseudotyped with lymphocytic choriomeningitis virus. J Virol 73, 6114–6116.
    [Google Scholar]
  40. Neubauer, A., Meindl, A. & Osterrieder, N. ( 1999; ). Mutations in the US2 and glycoprotein B genes of the equine herpesvirus 1 vaccine strain RacH have no effects on its attenuation. Berl Munch Tierarztl Wochenschr 112, 351–354.
    [Google Scholar]
  41. Nobiron, I., Thompson, I., Brownlie, J. & Collins, M. E. ( 2000; ). Co-administration of IL-2 enhances antigen-specific immune responses following vaccination with DNA encoding the glycoprotein E2 of bovine viral diarrhoea virus. Vet Microbiol 76, 129–142.[CrossRef]
    [Google Scholar]
  42. Nobiron, I., Thompson, I., Brownlie, J. & Collins, M. E. ( 2003; ). DNA vaccination against bovine viral diarrhoea virus induces humoral and cellular responses in cattle with evidence for protection against viral challenge. Vaccine 21, 2082–2092.[CrossRef]
    [Google Scholar]
  43. O'Connor, M., Peifer, M. & Bender, W. ( 1989; ). Construction of large DNA segments in Escherichia coli. Science 244, 1307–1312.[CrossRef]
    [Google Scholar]
  44. O'Connor, A. M., Sorden, S. D. & Apley, M. D. ( 2005; ). Association between the existence of calves persistently infected with bovine viral diarrhea virus and commingling on pen morbidity in feedlot cattle. Am J Vet Res 66, 2130–2134.[CrossRef]
    [Google Scholar]
  45. Osterrieder, N., Neubauer, A., Brandmuller, C., Kaaden, O. R. & O'Callaghan, D. J. ( 1996; ). The equine herpesvirus 1 IR6 protein influences virus growth at elevated temperature and is a major determinant of virulence. Virology 226, 243–251.[CrossRef]
    [Google Scholar]
  46. Osterrieder, N., Schumacher, D., Trapp, S., Beer, M., von Einem, J. & Tischer, K. ( 2003; ). Establishment and use of infectious bacterial artificial chromosome (BAC) DNA clones of animal herpesviruses. Berl Munch Tierarztl Wochenschr 116, 373–380.
    [Google Scholar]
  47. Piccinini, R., Luzzago, C., Frigerio, M., Frigerio, V., Liandris, E. & Zecconi, A. ( 2006; ). Comparison of blood non-specific immune parameters in Bovine virus diarrhoea virus (BVDV) persistently infected and in immune heifers. J Vet Med B Infect Dis Vet Public Health 53, 62–67.[CrossRef]
    [Google Scholar]
  48. Potgieter, L. N. ( 1995; ). Immunology of bovine viral diarrhea virus. Vet Clin North Am Food Anim Pract 11, 501–520.
    [Google Scholar]
  49. Purdy, C. W., Ford, S. J. & Porter, R. C. ( 1978; ). Equine rhinopneumonitis vaccine: immunogenicity and safety in adult horses, including pregnant mares. Am J Vet Res 39, 377–383.
    [Google Scholar]
  50. Reimann, I., Meyers, G. & Beer, M. ( 2003; ). Trans-complementation of autonomously replicating Bovine viral diarrhea virus replicons with deletions in the E2 coding region. Virology 307, 213–227.[CrossRef]
    [Google Scholar]
  51. Ridpath, J. F. & Bolin, S. R. ( 1995; ). Delayed onset postvaccinal mucosal disease as a result of genetic recombination between genotype 1 and genotype 2 BVDV. Virology 212, 259–262.[CrossRef]
    [Google Scholar]
  52. Rosas, C. T., Goodman, L. B., von Einem, J. & Osterrieder, N. ( 2006; ). Equine herpesvirus type 1 modified live virus vaccines: quo vaditis? Expert Rev Vaccines 5, 119–131.[CrossRef]
    [Google Scholar]
  53. Rudolph, J. & Osterrieder, N. ( 2002; ). Equine herpesvirus type 1 devoid of gM and gp2 is severely impaired in virus egress but not direct cell-to-cell spread. Virology 293, 356–367.[CrossRef]
    [Google Scholar]
  54. Rudolph, J., O'Callaghan, D. J. & Osterrieder, N. ( 2002; ). Cloning of the genomes of equine herpesvirus type 1 (EHV-1) strains KyA and racL11 as bacterial artificial chromosomes (BAC). J Vet Med B Infect Dis Vet Public Health 49, 31–36.[CrossRef]
    [Google Scholar]
  55. Saeki, Y. ( 2000; ). Advanced methods for the manipulation of herpesvirus genomes. Nippon Rinsho 58, 815–821.
    [Google Scholar]
  56. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  57. Telford, E. A., Watson, M. S., McBride, K. & Davison, A. J. ( 1992; ). The DNA sequence of equine herpesvirus-1. Virology 189, 304–316.[CrossRef]
    [Google Scholar]
  58. Thurmond, M. C., Munoz-Zanzi, C. A. & Hietala, S. K. ( 2001; ). Effect of calfhood vaccination on transmission of bovine viral diarrhea virus under typical drylot dairy conditions. J Am Vet Med Assoc 219, 968–975.[CrossRef]
    [Google Scholar]
  59. Trapp, S., von Einem, J., Hofmann, H., Kostler, J., Wild, J., Wagner, R., Beer, M. & Osterrieder, N. ( 2005; ). Potential of equine herpesvirus 1 as a vector for immunization. J Virol 79, 5445–5454.[CrossRef]
    [Google Scholar]
  60. Van Campen, H., Vorpahl, P., Huzurbazar, S., Edwards, J. & Cavender, J. ( 2000; ). A case report: evidence for type 2 bovine viral diarrhea virus (BVDV)-associated disease in beef herds vaccinated with a modified-live type 1 BVDV vaccine. J Vet Diagn Invest 12, 263–265.[CrossRef]
    [Google Scholar]
  61. Wagner, M., Jonjic, S., Koszinowski, U. H. & Messerle, M. ( 1999; ). Systematic excision of vector sequences from the BAC-cloned herpesvirus genome during virus reconstitution. J Virol 73, 7056–7060.
    [Google Scholar]
  62. Wagner, M., Ruzsics, Z. & Koszinowski, U. H. ( 2002; ). Herpesvirus genetics has come of age. Trends Microbiol 10, 318–324.[CrossRef]
    [Google Scholar]
  63. Wang, L., Sunyer, J. O. & Bello, L. J. ( 2004; ). Fusion to C3d enhances the immunogenicity of the E2 glycoprotein of type 2 bovine viral diarrhea virus. J Virol 78, 1616–1622.[CrossRef]
    [Google Scholar]
  64. Wolff, J. A. ( 2002; ). The ‘grand’ problem of synthetic delivery. Nat Biotechnol 20, 768–769.[CrossRef]
    [Google Scholar]
  65. Zimmer, G. M., Wentink, G. H., Bruschke, C., Westenbrink, F. J., Brinkhof, J. & de Goey, I. ( 2002; ). Failure of foetal protection after vaccination against an experimental infection with bovine virus diarrhea virus. Vet Microbiol 89, 255–265.[CrossRef]
    [Google Scholar]
  66. Zimmerman, A. D., Boots, R. E., Valli, J. L. & Chase, C. C. ( 2006; ). Evaluation of protection against virulent bovine viral diarrhea virus type 2 in calves that had maternal antibodies and were vaccinated with a modified-live vaccine. J Am Vet Med Assoc 228, 1757–1761.[CrossRef]
    [Google Scholar]
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vol. , part 3, pp. 748 – 757

Restriction fragment-length polymorphisms (RFLPs) and Southern blot

Vaccination of mice with rH_BVDV results in induction of serum neutralizing antibodies against the heterologous strain Singer

Vaccination of cattle with rH_BVDV results in a reduction of the BVDV type Ib challenge virus SE5508 replication

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