RT Journal Article SR Electronic(1) A1 Sherwood, Victoria A1 Burgert, Hans-Gerhard A1 Chen, Yun-Hsiang A1 Sanghera, Sandeep A1 Katafigiotis, Socrates A1 Randall, Richard E. A1 Connerton, Ian A1 Mellits, Kenneth H.YR 2007 T1 Improved growth of enteric adenovirus type 40 in a modified cell line that can no longer respond to interferon stimulation JF Journal of General Virology, VO 88 IS 1 SP 71 OP 76 DO https://doi.org/10.1099/vir.0.82445-0 PB Microbiology Society, SN 1465-2099, AB Human enteric adenoviruses propagate poorly in conventional human cell lines used to grow other adenovirus serotypes. As human enteric adenoviruses have a defect in counteracting the cellular interferon (IFN) response in cell culture, to aid in growth of the virus, a 293-based cell line defective in its ability to respond to IFN was constructed. This cell line (293-SV5/V) constitutively expresses V-protein of the paramyxovirus Simian virus 5, which degrades the signal transducer and activator of transcription 1 (STAT1) and thereby prevents the STAT1-mediated IFN response. Analysis of human enteric adenovirus type 40 (HAdV-40)-infected 293-SV5/V cells compared with parental 293 cells shows that the recombinant line allows more rapid production of virus and results in higher titres. These results suggest that the defect in HAdV-40 in counteracting the IFN response can be overcome at least partially through the use of 293-SV5/V cell lines., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.82445-0