1887

Abstract

Titration studies of the infectivity of experimental bovine spongiform encephalopathy (BSE) in sheep are necessary to assess the risk for human health posed by the ovine infection relative to the original cattle disease. Here, a comparative titration was performed of sheep-passaged BSE infectivity in Romney sheep and RIII mice, by the intracerebral (i.c.) and i.c. plus intraperitoneal (i.p.) routes, respectively. The sheep-to-mouse species barrier was lower than anticipated, as similar titres were obtained for both sheep [1×10 (i.c.) ID g)] and mice [1×10 (i.c.+i.p.) ID g]. Moreover, sheep of the ARR/ARR PrP genotype all succumbed to i.c. challenge with a 10 dilution of 0.5 g of a brainstem pool from BSE-affected sheep, indicating that resistance to natural infection in sheep of this genotype must reside in some mechanism of peripheral pathogenesis.

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2007-02-01
2024-12-07
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References

  1. Bellworthy S. J., Dexter G., Stack M., Chaplin M., Hawkins S. A. C., Simmons M. M., Jeffrey M., Martin S., González L., Hill P. 2005; Natural transmission of BSE between sheep within an experimental flock. Vet Rec 157:206 [CrossRef]
    [Google Scholar]
  2. Bradley R. 2001; Bovine spongiform encephalopathy and its relationship to the new variant form of Creutzfeldt–Jakob disease. In Prions. A Challenge for Science and Public Health System. Contributions to Microbiology vol. 7 pp  105–144 Edited by Rabeneau H. F., Cinatl J., Basel H. W. Doerr: Karger Publishers;
    [Google Scholar]
  3. Bruce M. E., Will R. G., Ironside J. W., McConell I., Drummond D., Suttie A., McCardle L., Chree A., Hope J. other authors 1997; Transmissions to mice indicate that ‘new variant’ CJD is caused by the BSE agent. Nature 389:498–501 [CrossRef]
    [Google Scholar]
  4. Foster J. D., Hope J., Fraser H. 1993; Transmission of bovine spongiform encephalopathy to sheep and goats. Vet Rec 133:339–341 [CrossRef]
    [Google Scholar]
  5. Fraser H., Dickinson A. G. 1973; Scrapie in mice: agent-strain differences in the distribution and intensity of grey matter vacuolation. J Comp Pathol 83:29–40 [CrossRef]
    [Google Scholar]
  6. Fraser H., Foster J. D. 1994; Transmission to mice, sheep and goats and bioassay of bovine tissues. In Transmissible Spongiform Encephalopathies. Proceedings of a Consultation with the Scientific Veterinary Committee of the CEC pp  145–149 Edited by Bradley R., Marchant B. VI/413/94-EN Brussels, EC:
    [Google Scholar]
  7. Fraser H., McConnell I., Wells G. A. H., Dawson M. 1988; Transmission of bovine spongiform encephalopathy to mice. Vet Rec 123:472 [CrossRef]
    [Google Scholar]
  8. Fraser H., Bruce M. E., Chree A., McConnell I., Wells G. A. H. 1992; Transmission of bovine spongiform encephalopathy and scrapie to mice. J Gen Virol 73:1891–1897 [CrossRef]
    [Google Scholar]
  9. González L., Martin S., Begara-McGorum I., Hunter N., Houston F., Simmons M., Jeffrey M. 2002; Effects of agent strain and host genotype on PrP accumulation in the brain of sheep naturally and experimentally affected with scrapie. J Comp Pathol 126:17–29 [CrossRef]
    [Google Scholar]
  10. González L., Martin S., Houston F. E., Hunter N., Reid H. W., Bellworthy S. J., Jeffrey M. 2005a; Phenotype of disease-associated PrP accumulation in the brain of bovine spongiform encephalopathy experimentally infected sheep. J Gen Virol 86:827–838 [CrossRef]
    [Google Scholar]
  11. González L., Terry L., Jeffrey M. 2005b; Expression of prion protein in the gut of mice infected orally with the 301V murine strain of the bovine spongiform encephalopathy agent. J Comp Pathol 132:273–282 [CrossRef]
    [Google Scholar]
  12. Hamilton M. A., Russo R. C., Thurston R. V. 1977; Trimmed Spearman–Karber method for estimating median letal concentrations in toxicity bioassays. Environ Sci Technol 11:714–719 [CrossRef]
    [Google Scholar]
  13. Jeffrey M., González L., Espenes A., Press C. M., Martin S., Chaplin M., Davis L., Landsverk T., MacAldowie C. other authors 2006; Transportation of prion protein across the intestinal mucosa of scrapie-susceptible and scrapie-resistant sheep. J Pathol 209:4–14 [CrossRef]
    [Google Scholar]
  14. Stack M., Jeffrey M., Gubbins S., Grimmer S., González L., Martin S., Chaplin M., Webb P., Simmons M. other authors 2006; Monitoring for bovine spongiform encephalopathy in sheep in Great Britain, 1998–2004. J Gen Virol 87:2099–2107 [CrossRef]
    [Google Scholar]
  15. Wells G. A. H., Scott A. C., Johnson C. T., Gunning R. F., Hancock R. D., Jeffrey M., Dawson M., Bradley R. 1987; A novel progressive spongiform encephalopathy in cattle. Vet Rec 121:419–420 [CrossRef]
    [Google Scholar]
  16. Will R. G., Ironside J. W., Zeidler M., Cousens S. N., Estibeiro K., Alperovitch A., Poser S., Pocchiari M., Hofman A., Smith P. G. 1996; A new variant of Creutzfeldt–Jakob disease in the UK. Lancet 347:921–925 [CrossRef]
    [Google Scholar]
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