1887

Abstract

By analysing the antigenic structure of the morbillivirus nucleoprotein (N) using a competitive-binding assay of monoclonal antibodies (mAbs), six different antigenic sites were identified previously. By using Pepscan methodology complemented by analysis of truncated N proteins, a better characterization of five of these antigenic sites was provided: I, II, III, IV and VI. mAbs specific to , defining antigenic sites II, III and IV, and those common to four morbilliviruses, delineating sites I and VI, were analysed in the present study. It was found that all but one mapped to the same region, between aa 120 and 149 of N. However, the mAb 3-1 epitope was located in the carboxy-terminal region (aa 421–525). This result may indicate the high immunogenicity of the amino-terminal variable region, at least in the mouse. It was surprising that the epitope of mAb 33-4, antigenic site VI, which recognized all morbilliviruses so far tested, was located in one of the two non-conserved regions between morbillivirus N proteins. It is shown that the conserved amino acid motif EAD----F-------EN is critical for epitope constitution and recognition.

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2007-04-01
2019-11-13
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References

  1. Ailenberg, M. & Silverman, M. ( 1997; ). Site-directed mutagenesis using a PCR-based staggered re-annealing method without restriction enzymes. Biotechniques 22, 624–630.
    [Google Scholar]
  2. Alba, M. P., Salazar, L. M., Puentes, A., Pinto, M., Torres, E. & Patarroyo, M. E. ( 2003; ). 6746 SERA peptide analogues immunogenicity and protective efficacy against malaria is associated with short alpha helix formation: malaria protection associated with peptides alpha helix shortening. Peptides 24, 999–1006.[CrossRef]
    [Google Scholar]
  3. Anderson, J., McKay, J. A. & Butcher, R. N. ( 1990; ). The use of monoclonal antibodies in the competitive ELISA for the detection of antibodies to rinderpest and peste des petits ruminants viruses. In The Seromonitoring of Rinderpest throughout Africa – Phase One (Proceedings of the Final Research Coordination Meeting of the FAO/IAEA/SIDA/OUA/IBAR/PARC Coordinated Research programme). IAEA-TECDOC vol. 623, pp. 43–45. Edited by M. H. Jeggo. Vienna: Joint FAO/IAEA Division.
  4. Baldi, P., Brunak, S., Frasconi, P., Soda, G. & Pollastri, G. ( 1999; ). Exploiting the past and the future in protein secondary structure prediction. Bioinformatics 15, 937–946.[CrossRef]
    [Google Scholar]
  5. Barrett, T., Banyard, A. & Diallo, A. ( 2006; ). Molecular biology of the morbilliviruses. In Rinderpest and Peste des Petits Ruminants, pp. 31–67. Edited by T. Barrett, P.-P. Pastoret & W. Taylor. London: Elsevier Academic Press.
  6. Bratt, M. A. & Hightower, L. E. ( 1977; ). Genetics and paragenetic phenomena of paramyxoviruses. In Comprehensive Virology, vol. 9, pp. 457–533. Edited by H. Frankel-Conrat & R. R. Wagner. New York: Plenum.
  7. Buckland, R., Giraudon, P. & Wild, F. ( 1989; ). Expression of measles virus nucleoprotein in Escherichia coli: use of deletion mutants to locate the antigenic sites. J Gen Virol 70, 435–441.[CrossRef]
    [Google Scholar]
  8. Choi, K. S., Kwon, C. H., Choi, C. U., Lee, J. G. & Kang, Y. B. ( 1998; ). Biological properties of attenuated rinderpest virus (LATC strain) adapted in Vero cell. RDA J Vet Sci 40, 61–70.
    [Google Scholar]
  9. Choi, K. S., Nah, J. J., Ko, Y. J., Choi, C. U., Kim, J. H., Kang, S. Y. & Joo, Y. S. ( 2003a; ). Characterization of antigenic sites on the rinderpest virus N protein using monoclonal antibodies. J Vet Sci 4, 57–65.
    [Google Scholar]
  10. Choi, K. S., Nah, J. J., Ko, Y. J., Kang, S. Y. & Joo, Y. S. ( 2003b; ). Localization of antigenic sites at the amino-terminus of rinderpest virus N protein using deleted N mutants and monoclonal antibody. J Vet Sci 4, 167–173.
    [Google Scholar]
  11. Choi, K. S., Nah, J. J., Ko, Y. J., Kang, S. Y., Yoon, K. J. & Joo, Y. S. ( 2004; ). Characterization of immunodominant linear B-cell epitopes on the carboxy terminus of the rinderpest virus nucleocapsid protein. Clin Diagn Lab Immunol 11, 658–664.
    [Google Scholar]
  12. Choppin, P. W. & Scheid, A. ( 1980; ). The role of viral glycoproteins in adsorption, penetration, and pathogenicity of viruses. Rev Infect Dis 2, 40–61.[CrossRef]
    [Google Scholar]
  13. Cuff, J. A. & Barton, G. J. ( 2000; ). Application of multiple sequence alignment profiles to improve protein secondary structure prediction. Proteins 40, 502–511.[CrossRef]
    [Google Scholar]
  14. Diallo, A., Barrett, T., Barbron, M., Meyer, G. & Lefevre, P. C. ( 1994; ). Cloning of the nucleocapsid protein gene of peste-des-petits-ruminants virus: relationship to other morbilliviruses. J Gen Virol 75, 233–237.[CrossRef]
    [Google Scholar]
  15. Frishman, D. & Argos, P. ( 1996; ). Incorporation of non-local interactions in protein secondary structure prediction from the amino acid sequence. Protein Eng 9, 133–142.[CrossRef]
    [Google Scholar]
  16. Garnier, J., Gibrat, J.-F. & Robson, B. ( 1996; ). gor method for predicting protein secondary structure from amino acid sequence. Methods Enzymol 266, 540–553.
    [Google Scholar]
  17. Giraudon, P. & Wild, T. F. ( 1985; ). Correlation between epitopes on hemagglutinin of measles virus and biological activities: passive protection by monoclonal antibodies is related to their haemagglutination inhibiting activity. Virology 144, 46–58.[CrossRef]
    [Google Scholar]
  18. Giraudon, P., Jacquier, M. F. & Wild, T. F. ( 1988; ). Antigenic analysis of African measles virus field isolates: identification and localisation of one conserved and two variable epitope sites on the NP protein. Virus Res 10, 137–152.[CrossRef]
    [Google Scholar]
  19. Heaney, J., Barrett, T. & Cosby, S. L. ( 2002; ). Inhibition of in vitro leukocyte proliferation by morbilliviruses. J Virol 76, 3579–3584.[CrossRef]
    [Google Scholar]
  20. Heaney, J., Cosby, S. L. & Barrett, T. ( 2005; ). Inhibition of host peripheral blood mononuclear cell proliferation ex vivo by Rinderpest virus. J Gen Virol 86, 3349–3355.[CrossRef]
    [Google Scholar]
  21. Jones, D. T. ( 1999; ). Protein secondary structure prediction based on position-specific scoring matrices. J Mol Biol 292, 195–202.[CrossRef]
    [Google Scholar]
  22. Kamata, H., Tsukiyama, K., Sugiyama, M., Kamata, Y., Yoshikawa, Y. & Yamanouchi, K. ( 1991; ). Nucleotide sequence of cDNA to the rinderpest virus mRNA encoding the nucleocapsid protein. Virus Genes 5, 5–15.[CrossRef]
    [Google Scholar]
  23. Karlin, D., Longhi, S. & Canard, B. ( 2002; ). Substitution of two residues in the measles virus nucleoprotein results in an impaired self-association. Virology 302, 420–432.[CrossRef]
    [Google Scholar]
  24. Kerdiles, Y. M., Cherif, B., Marie, J. C., Tremillon, N., Blanquier, B., Libeau, G., Diallo, A., Wild, T. F., Villiers, M. B. & Horvat, B. ( 2006; ). Immunomodulatory properties of morbillivirus nucleoproteins. Viral Immunol 19, 324–334.[CrossRef]
    [Google Scholar]
  25. Laine, D., Trescol-Biemont, M. C., Longhi, S., Libeau, G., Marie, J. C., Vidalain, P. O., Azocar, O., Diallo, A., Canard, B. & other authors ( 2003; ). Measles virus (MV) nucleoprotein binds to a novel cell surface receptor distinct from FcγRII via its C-terminal domain: role in MV-induced immunosuppression. J Virol 77, 11332–11346.[CrossRef]
    [Google Scholar]
  26. Libeau, G., Diallo, A., Calvez, D. & Lefevre, P. C. ( 1992; ). A competitive ELISA using anti-N monoclonal antibodies for specific detection of rinderpest antibodies in cattle and small ruminants. Vet Microbiol 31, 147–160.[CrossRef]
    [Google Scholar]
  27. Libeau, G., Diallo, A., Colas, F. & Guerre, L. ( 1994; ). Rapid differential diagnosis of rinderpest and peste des petits ruminants using an immunocapture ELISA. Vet Rec 134, 300–304.[CrossRef]
    [Google Scholar]
  28. Libeau, G., Prehaud, C., Lancelot, R., Colas, F., Guerre, L., Bishop, D. H. & Diallo, A. ( 1995; ). Development of a competitive ELISA for detecting antibodies to the peste des petits ruminants virus using a recombinant nucleoprotein. Res Vet Sci 58, 50–55.[CrossRef]
    [Google Scholar]
  29. Libeau, G., Saliki, J. T. & Diallo, A. ( 1997; ). Caractérisation d’anticorps monoclonaux dirigés contre les virus de la peste bovine et de la peste des petits ruminants: identification d’épitopes conservés ou de spécificité stricte sur la nucléoprotéine. Rev Elev Med Vet Pays Trop 50, 181–190. (in French).
    [Google Scholar]
  30. Liston, P., Batal, R., DiFlumeri, C. & Briedis, D. J. ( 1997; ). Protein interaction domains of the measles virus nucleocapsid protein (NP). Arch Virol 142, 305–321.[CrossRef]
    [Google Scholar]
  31. Longhi, S., Receveur-Brechot, V., Karlin, D., Johansson, K., Darbon, H., Bhella, D., Yeo, R., Finet, S. & Canard, B. ( 2003; ). The C-terminal domain of the measles virus nucleoprotein is intrinsically disordered and folds upon binding to the C-terminal moiety of the phosphoprotein. J Biol Chem 278, 18638–18648.[CrossRef]
    [Google Scholar]
  32. Mahé, D., Blanchard, P., Truong, C., Arnauld, C., Le Cann, P., Cariolet, R., Madec, F., Albina, E. & Jestin, A. ( 2000; ). Differential recognition of ORF2 protein from type 1 and type 2 porcine circoviruses and identification of immunorelevant epitopes. J Gen Virol 81, 1815–1824.
    [Google Scholar]
  33. Merz, D. C., Scheid, A. & Choppin, P. W. ( 1981; ). Immunological studies of the functions of paramyxovirus glycoproteins. Virology 109, 94–105.[CrossRef]
    [Google Scholar]
  34. Plowright, W. ( 1962; ). Rinderpest virus. Ann N Y Acad Sci 101, 548–573.
    [Google Scholar]
  35. Plowright, W. ( 1968; ). Rinderpest virus. In Virology Monographs vol. 3, pp. 26–110. Edited by S. Gard, C. Hallauer & K. F. Meyer. New York: Springer-Verlag.
  36. Rost, B. ( 2001; ). Protein secondary structure prediction continues to rise. J Struct Biol 134, 204–218.
    [Google Scholar]
  37. Taylor, W. P. ( 1986; ). Epidemiology and control of rinderpest. Rev Sci Tech Off Int Epizoot 5, 407–410.
    [Google Scholar]
  38. tenOever, B. R., Servant, M. J., Grandvaux, N., Lin, R. & Hiscott, J. ( 2002; ). Recognition of the measles virus nucleocapsid as a mechanism of IRF-3 activation. J Virol 76, 3659–3669.[CrossRef]
    [Google Scholar]
  39. Zhang, X., Glendening, C., Linke, H., Parks, C. L., Brooks, C., Udem, S. A. & Oglesbee, M. ( 2002; ). Identification and characterization of a regulatory domain on the carboxyl terminus of the measles virus nucleocapsid protein. J Virol 76, 8737–8746.[CrossRef]
    [Google Scholar]
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