1887

Abstract

By analysing the antigenic structure of the morbillivirus nucleoprotein (N) using a competitive-binding assay of monoclonal antibodies (mAbs), six different antigenic sites were identified previously. By using Pepscan methodology complemented by analysis of truncated N proteins, a better characterization of five of these antigenic sites was provided: I, II, III, IV and VI. mAbs specific to , defining antigenic sites II, III and IV, and those common to four morbilliviruses, delineating sites I and VI, were analysed in the present study. It was found that all but one mapped to the same region, between aa 120 and 149 of N. However, the mAb 3-1 epitope was located in the carboxy-terminal region (aa 421–525). This result may indicate the high immunogenicity of the amino-terminal variable region, at least in the mouse. It was surprising that the epitope of mAb 33-4, antigenic site VI, which recognized all morbilliviruses so far tested, was located in one of the two non-conserved regions between morbillivirus N proteins. It is shown that the conserved amino acid motif EAD----F-------EN is critical for epitope constitution and recognition.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.82424-0
2007-04-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/88/4/1231.html?itemId=/content/journal/jgv/10.1099/vir.0.82424-0&mimeType=html&fmt=ahah

References

  1. Ailenberg M., Silverman M. 1997; Site-directed mutagenesis using a PCR-based staggered re-annealing method without restriction enzymes. Biotechniques 22:624–630
    [Google Scholar]
  2. Alba M. P., Salazar L. M., Puentes A., Pinto M., Torres E., Patarroyo M. E. 2003; 6746 SERA peptide analogues immunogenicity and protective efficacy against malaria is associated with short alpha helix formation: malaria protection associated with peptides alpha helix shortening. Peptides 24:999–1006 [CrossRef]
    [Google Scholar]
  3. Anderson J., McKay J. A., Butcher R. N. 1990; The use of monoclonal antibodies in the competitive ELISA for the detection of antibodies to rinderpest and peste des petits ruminants viruses. In The Seromonitoring of Rinderpest throughout Africa – Phase One (Proceedings of the Final Research Coordination Meeting of the FAO/IAEA/SIDA/OUA/IBAR/PARC Coordinated Research programme) IAEA-TECDOC vol 623 pp. 43–45 Edited by Jeggo M. H. Vienna: Joint FAO/IAEA Division;
    [Google Scholar]
  4. Baldi P., Brunak S., Frasconi P., Soda G., Pollastri G. 1999; Exploiting the past and the future in protein secondary structure prediction. Bioinformatics 15:937–946 [CrossRef]
    [Google Scholar]
  5. Barrett T., Banyard A., Diallo A. 2006; Molecular biology of the morbilliviruses. In Rinderpest and Peste des Petits Ruminants . pp 31–67 Edited by Barrett T., Pastoret P.-P., Taylor W. London: Elsevier Academic Press;
  6. Bratt M. A., Hightower L. E. 1977; Genetics and paragenetic phenomena of paramyxoviruses. In Comprehensive Virology , vol. 9 pp 457–533 Edited by Frankel-Conrat H., Wagner R. R. New York: Plenum;
    [Google Scholar]
  7. Buckland R., Giraudon P., Wild F. 1989; Expression of measles virus nucleoprotein in Escherichia coli : use of deletion mutants to locate the antigenic sites. J Gen Virol 70:435–441 [CrossRef]
    [Google Scholar]
  8. Choi K. S., Kwon C. H., Choi C. U., Lee J. G., Kang Y. B. 1998; Biological properties of attenuated rinderpest virus (LATC strain) adapted in Vero cell. RDA J Vet Sci 40:61–70
    [Google Scholar]
  9. Choi K. S., Nah J. J., Ko Y. J., Choi C. U., Kim J. H., Kang S. Y., Joo Y. S. 2003a; Characterization of antigenic sites on the rinderpest virus N protein using monoclonal antibodies. J Vet Sci 4:57–65
    [Google Scholar]
  10. Choi K. S., Nah J. J., Ko Y. J., Kang S. Y., Joo Y. S. 2003b; Localization of antigenic sites at the amino-terminus of rinderpest virus N protein using deleted N mutants and monoclonal antibody. J Vet Sci 4:167–173
    [Google Scholar]
  11. Choi K. S., Nah J. J., Ko Y. J., Kang S. Y., Yoon K. J., Joo Y. S. 2004; Characterization of immunodominant linear B-cell epitopes on the carboxy terminus of the rinderpest virus nucleocapsid protein. Clin Diagn Lab Immunol 11:658–664
    [Google Scholar]
  12. Choppin P. W., Scheid A. 1980; The role of viral glycoproteins in adsorption, penetration, and pathogenicity of viruses. Rev Infect Dis 2:40–61 [CrossRef]
    [Google Scholar]
  13. Cuff J. A., Barton G. J. 2000; Application of multiple sequence alignment profiles to improve protein secondary structure prediction. Proteins 40:502–511 [CrossRef]
    [Google Scholar]
  14. Diallo A., Barrett T., Barbron M., Meyer G., Lefevre P. C. 1994; Cloning of the nucleocapsid protein gene of peste-des-petits-ruminants virus: relationship to other morbilliviruses. J Gen Virol 75:233–237 [CrossRef]
    [Google Scholar]
  15. Frishman D., Argos P. 1996; Incorporation of non-local interactions in protein secondary structure prediction from the amino acid sequence. Protein Eng 9:133–142 [CrossRef]
    [Google Scholar]
  16. Garnier J., Gibrat J.-F., Robson B. 1996; gor method for predicting protein secondary structure from amino acid sequence. Methods Enzymol 266:540–553
    [Google Scholar]
  17. Giraudon P., Wild T. F. 1985; Correlation between epitopes on hemagglutinin of measles virus and biological activities: passive protection by monoclonal antibodies is related to their haemagglutination inhibiting activity. Virology 144:46–58 [CrossRef]
    [Google Scholar]
  18. Giraudon P., Jacquier M. F., Wild T. F. 1988; Antigenic analysis of African measles virus field isolates: identification and localisation of one conserved and two variable epitope sites on the NP protein. Virus Res 10:137–152 [CrossRef]
    [Google Scholar]
  19. Heaney J., Barrett T., Cosby S. L. 2002; Inhibition of in vitro leukocyte proliferation by morbilliviruses. J Virol 76:3579–3584 [CrossRef]
    [Google Scholar]
  20. Heaney J., Cosby S. L., Barrett T. 2005; Inhibition of host peripheral blood mononuclear cell proliferation ex vivo by Rinderpest virus . J Gen Virol 86:3349–3355 [CrossRef]
    [Google Scholar]
  21. Jones D. T. 1999; Protein secondary structure prediction based on position-specific scoring matrices. J Mol Biol 292:195–202 [CrossRef]
    [Google Scholar]
  22. Kamata H., Tsukiyama K., Sugiyama M., Kamata Y., Yoshikawa Y., Yamanouchi K. 1991; Nucleotide sequence of cDNA to the rinderpest virus mRNA encoding the nucleocapsid protein. Virus Genes 5:5–15 [CrossRef]
    [Google Scholar]
  23. Karlin D., Longhi S., Canard B. 2002; Substitution of two residues in the measles virus nucleoprotein results in an impaired self-association. Virology 302:420–432 [CrossRef]
    [Google Scholar]
  24. Kerdiles Y. M., Cherif B., Marie J. C., Tremillon N., Blanquier B., Libeau G., Diallo A., Wild T. F., Villiers M. B., Horvat B. 2006; Immunomodulatory properties of morbillivirus nucleoproteins. Viral Immunol 19:324–334 [CrossRef]
    [Google Scholar]
  25. Laine D., Trescol-Biemont M. C., Longhi S., Libeau G., Marie J. C., Vidalain P. O., Azocar O., Diallo A., Canard B. other authors 2003; Measles virus (MV) nucleoprotein binds to a novel cell surface receptor distinct from Fc γ RII via its C-terminal domain: role in MV-induced immunosuppression. J Virol 77:11332–11346 [CrossRef]
    [Google Scholar]
  26. Libeau G., Diallo A., Calvez D., Lefevre P. C. 1992; A competitive ELISA using anti-N monoclonal antibodies for specific detection of rinderpest antibodies in cattle and small ruminants. Vet Microbiol 31:147–160 [CrossRef]
    [Google Scholar]
  27. Libeau G., Diallo A., Colas F., Guerre L. 1994; Rapid differential diagnosis of rinderpest and peste des petits ruminants using an immunocapture ELISA. Vet Rec 134:300–304 [CrossRef]
    [Google Scholar]
  28. Libeau G., Prehaud C., Lancelot R., Colas F., Guerre L., Bishop D. H., Diallo A. 1995; Development of a competitive ELISA for detecting antibodies to the peste des petits ruminants virus using a recombinant nucleoprotein. Res Vet Sci 58:50–55 [CrossRef]
    [Google Scholar]
  29. Libeau G., Saliki J. T., Diallo A. 1997; Caractérisation d’anticorps monoclonaux dirigés contre les virus de la peste bovine et de la peste des petits ruminants: identification d’épitopes conservés ou de spécificité stricte sur la nucléoprotéine. Rev Elev Med Vet Pays Trop 50:181–190 (in French
    [Google Scholar]
  30. Liston P., Batal R., DiFlumeri C., Briedis D. J. 1997; Protein interaction domains of the measles virus nucleocapsid protein (NP. Arch Virol 142:305–321 [CrossRef]
    [Google Scholar]
  31. Longhi S., Receveur-Brechot V., Karlin D., Johansson K., Darbon H., Bhella D., Yeo R., Finet S., Canard B. 2003; The C-terminal domain of the measles virus nucleoprotein is intrinsically disordered and folds upon binding to the C-terminal moiety of the phosphoprotein. J Biol Chem 278:18638–18648 [CrossRef]
    [Google Scholar]
  32. Mahé D., Blanchard P., Truong C., Arnauld C., Le Cann P., Cariolet R., Madec F., Albina E., Jestin A. 2000; Differential recognition of ORF2 protein from type 1 and type 2 porcine circoviruses and identification of immunorelevant epitopes. J Gen Virol 81:1815–1824
    [Google Scholar]
  33. Merz D. C., Scheid A., Choppin P. W. 1981; Immunological studies of the functions of paramyxovirus glycoproteins. Virology 109:94–105 [CrossRef]
    [Google Scholar]
  34. Plowright W. 1962; Rinderpest virus. Ann N Y Acad Sci 101:548–573
    [Google Scholar]
  35. Plowright W. 1968; Rinderpest virus. In Virology Monographs vol 3 pp 26–110 Edited by Gard S., Hallauer C., Meyer K. F. New York: Springer-Verlag;
    [Google Scholar]
  36. Rost B. 2001; Protein secondary structure prediction continues to rise. J Struct Biol 134:204–218
    [Google Scholar]
  37. Taylor W. P. 1986; Epidemiology and control of rinderpest. Rev Sci Tech Off Int Epizoot 5:407–410
    [Google Scholar]
  38. tenOever B. R., Servant M. J., Grandvaux N., Lin R., Hiscott J. 2002; Recognition of the measles virus nucleocapsid as a mechanism of IRF-3 activation. J Virol 76:3659–3669 [CrossRef]
    [Google Scholar]
  39. Zhang X., Glendening C., Linke H., Parks C. L., Brooks C., Udem S. A., Oglesbee M. 2002; Identification and characterization of a regulatory domain on the carboxyl terminus of the measles virus nucleocapsid protein. J Virol 76:8737–8746 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.82424-0
Loading
/content/journal/jgv/10.1099/vir.0.82424-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error