@article{mbs:/content/journal/jgv/10.1099/vir.0.82387-0, author = "Sawiris, G. Peter and Becker, Kevin G. and Elliott, Ellen J. and Moulden, Robert and Rohwer, Robert G.", title = "Molecular analysis of bovine spongiform encephalopathy infection by cDNA arrays", journal= "Journal of General Virology", year = "2007", volume = "88", number = "4", pages = "1356-1362", doi = "https://doi.org/10.1099/vir.0.82387-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.82387-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Here, the first cDNA array analysis of differential gene expression in bovine spongiform encephalopathy (BSE) is reported, using a spotted cDNA array platform representing nearly 17 000 mouse genes. Array analysis identified 296 gene candidates for differential expression in brain tissue from VM mice in late-stage infection with the 301V strain of BSE, compared with brain tissue from normal, age-matched VM mice. Real-time PCR confirmed differential expression of 25 of 31 genes analysed. Some of the genes identified by array analysis as being expressed differentially are associated with ubiquitin/proteasome function, lysosomal function, molecular chaperoning of protein folding or apoptosis. Other genes are involved in calcium ion binding/homeostasis, zinc ion binding/homeostasis or regulation of transcription. Principal-component analysis shows that the global gene-expression profiles of the BSE-infected samples have gene-expression signatures that are markedly different from, and completely non-overlapping with, those obtained from the normal controls.", }