1887

Abstract

The presence of homologous repeat () regions in multiple locations within baculovirus genomes has led to the hypothesis that they represent origins of DNA replication. This hypothesis has been supported by transient replication assays where plasmids carrying s replicated in the presence of virus DNA replication. This study investigated whether any specific region was essential for viral DNA replication , by generating a series of recombinant Autographa californica multiple nucleopolyhedrovirus where the gene replaced , , , , or . In addition, a double- knockout virus was constructed where both and were deleted. The successful construction of these knockout viruses indicated that no specific region was essential for virus production. These recombinant viruses were characterized by titrations of budded virus, expression of a variety of virus-specific proteins and the synthesis of viral DNA at various times after infection. The results demonstrated that each was dispensable for all of these properties and that no single region was absolutely essential for virus replication in cell culture. The functional significance of multiple origin regions is still unclear.

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2007-01-01
2019-09-23
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