1887

Abstract

(ACLSV) is the type species of the genus and its single-stranded, plus-sense RNA genome encodes a 216 kDa protein (P216) involved in replication, a 50 kDa movement protein (P50) and a 21 kDa coat protein (CP). In this study, it was investigated whether these proteins might have RNA silencing-suppressor activities by -mediated transient assay in the green fluorescent protein-expressing line 16c. The results indicated that none of these proteins could suppress local silencing in infiltrated leaves. However, systemic silencing in upper leaves induced by both single- and double-stranded RNA could be suppressed by P50, but not by a frame-shift mutant of P50, P216 or CP. Moreover, when P50 was expressed separately from where silencing signals were generated in a leaf, systemic silencing in upper leaves was inhibited. Collectively, our data indicate that P50 acts as a suppressor of systemic silencing without interfering with local silencing, probably by inhibiting the movement of silencing signals.

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2007-01-01
2019-10-24
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References

  1. Anandalakshmi, R., Pruss, G. J., Ge, X., Marathe, R., Mallory, A. C., Smith, T. H. & Vance, V. B. ( 1998; ). A viral suppressor of gene silencing in plants. Proc Natl Acad Sci U S A 95, 13079–13084.[CrossRef]
    [Google Scholar]
  2. Baulcombe, D. C. ( 2004; ). RNA silencing in plants. Nature 431, 356–363.[CrossRef]
    [Google Scholar]
  3. Brigneti, G., Voinnet, O., Li, W. X., Ji, L. H., Ding, S. W. & Baulcombe, D. C. ( 1998; ). Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana. EMBO J 17, 6739–6746.[CrossRef]
    [Google Scholar]
  4. Cao, X., Zhou, P., Zhang, X., Zhu, S., Zhong, X., Xiao, Q., Ding, B. & Li, Y. ( 2005; ). Identification of an RNA silencing suppressor from a plant double-stranded RNA virus. J Virol 79, 13018–13027.[CrossRef]
    [Google Scholar]
  5. Chapman, E. J., Prokhnevsky, A. I., Gopinath, K., Dolja, V. V. & Carrington, J. C. ( 2004; ). Viral RNA silencing suppressors inhibit the microRNA pathway at an intermediate step. Genes Dev 18, 1179–1186.[CrossRef]
    [Google Scholar]
  6. Cogoni, C. ( 2001; ). Homology-dependent gene silencing mechanisms in fungi. Annu Rev Microbiol 55, 381–406.[CrossRef]
    [Google Scholar]
  7. Ding, S. W., Li, H. W., Lu, R., Li, F. & Li, W. X. ( 2004; ). RNA silencing: a conserved antiviral immunity of plants and animals. Virus Res 102, 109–115.[CrossRef]
    [Google Scholar]
  8. Dunoyer, P., Lecellier, C. H., Parizotto, E. A., Himber, C. & Voinnet, O. ( 2004; ). Probing the microRNA and small interfering RNA pathways with virus-encoded suppressors of RNA silencing. Plant Cell 16, 1235–1250.[CrossRef]
    [Google Scholar]
  9. German, S., Candresse, T., Lanneau, M., Huet, J. C., Pernollet, J. C. & Dunez, J. ( 1990; ). Nucleotide sequence and genomic organization of apple chlorotic leaf spot closterovirus. Virology 179, 104–112.[CrossRef]
    [Google Scholar]
  10. German-Retana, S., Bergey, B., Delbos, R. P., Candresse, T. & Dunez, J. ( 1997; ). Complete nucleotide sequence of the genome of a severe cherry isolate of apple chlorotic leaf spot trichovirus (ACLSV). Arch Virol 142, 833–841.[CrossRef]
    [Google Scholar]
  11. Guo, H. S. & Ding, S. W. ( 2002; ). A viral protein inhibits the long range signaling activity of the gene silencing signal. EMBO J 21, 398–407.[CrossRef]
    [Google Scholar]
  12. Hamilton, A. J. & Baulcombe, D. C. ( 1999; ). A species of small antisense RNA in posttranscriptional gene silencing in plants. Science 286, 950–952.[CrossRef]
    [Google Scholar]
  13. Hamilton, A., Voinnet, O., Chappell, L. & Baulcombe, D. ( 2002; ). Two classes of short interfering RNA in RNA silencing. EMBO J 21, 4671–4679.[CrossRef]
    [Google Scholar]
  14. Hammond, S. M., Bernstein, E., Beach, D. & Hannon, G. J. ( 2000; ). An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature 404, 293–296.[CrossRef]
    [Google Scholar]
  15. Hannon, G. J. ( 2002; ). RNA interference. Nature 418, 244–251.[CrossRef]
    [Google Scholar]
  16. Himber, C., Dunoyer, P., Moissiard, G., Ritzenthaler, C. & Voinnet, O. ( 2003; ). Transitivity-dependent and -independent cell-to-cell movement of RNA silencing. EMBO J 22, 4523–4533.[CrossRef]
    [Google Scholar]
  17. Isogai, M. & Yoshikawa, N. ( 2005; ). Mapping the RNA-binding domain on the Apple chlorotic leaf spot virus movement protein. J Gen Virol 86, 225–229.[CrossRef]
    [Google Scholar]
  18. Isogai, M., Saitou, Y., Takahashi, N., Itabashi, T., Terada, M., Satoh, H. & Yoshikawa, N. ( 2003; ). The 50-kDa protein of Apple chlorotic leaf spot virus interferes with intracellular and intercellular targeting and tubule-inducing activity of the 39-kDa protein of Grapevine berry inner necrosis virus. Mol Plant Microbe Interact 16, 188–195.[CrossRef]
    [Google Scholar]
  19. Kasschau, K. D. & Carrington, J. C. ( 1998; ). A counterdefensive strategy of plant viruses: suppression of posttranscriptional gene silencing. Cell 95, 461–470.[CrossRef]
    [Google Scholar]
  20. Kobori, T., Osaki, T. & Ohki, S. T. ( 2003; ). Cucumber mosaic virus establishes systemic infection at increased temperature following viral entrance into the phloem pathway of Tetragonia expansa. Phytopathology 93, 1445–1451.[CrossRef]
    [Google Scholar]
  21. Lakatos, L., Szittya, G., Silhavy, D. & Burgyan, J. ( 2004; ). Molecular mechanism of RNA silencing suppression mediated by p19 protein of tombusviruses. EMBO J 23, 876–884.[CrossRef]
    [Google Scholar]
  22. Lister, R. M. ( 1970; ). Apple chlorotic leaf spot virus (CMI/AAB Description of Plant Viruses no. 30). Kew, UK: CMI/AAB.
  23. Lu, R., Folimonov, A., Shintaku, M., Li, W. X., Falk, B. W., Dawson, W. O. & Ding, S.-W. ( 2004; ). Three distinct suppressors of RNA silencing encoded by a 20-kb viral RNA genome. Proc Natl Acad Sci U S A 101, 15742–15747.[CrossRef]
    [Google Scholar]
  24. Martelli, G. P., Candresse, T. & Namba, S. ( 1994; ). Trichovirus, a new genus of plant viruses. Arch Virol 134, 451–455.[CrossRef]
    [Google Scholar]
  25. Mitsuhara, I., Ugaki, M., Hirochika, H., Ohshima, M., Murakami, T., Gotoh, Y., Katayose, Y., Nakamura, S., Honkura, R. & other authors ( 1996; ). Efficient promoter cassettes for enhanced expression of foreign genes in dicotyledonous and monocotyledonous plants. Plant Cell Physiol 37, 49–59.[CrossRef]
    [Google Scholar]
  26. Mlotshwa, S., Voinnet, O., Mette, M. F., Matzke, M., Vaucheret, H., Ding, S. W., Pruss, G. & Vance, V. B. ( 2002; ). RNA silencing and the mobile silencing signal. Plant Cell 14 (Suppl.), S289–S301.[CrossRef]
    [Google Scholar]
  27. Mushegian, A. R. & Koonin, E. V. ( 1993; ). Cell-to-cell movement of plant viruses. Insights from amino acid sequence comparisons of movement proteins and from analogies with cellular transport systems. Arch Virol 133, 239–257.[CrossRef]
    [Google Scholar]
  28. Palauqui, J. C., Elmayan, T., Pollien, J. M. & Vaucheret, H. ( 1997; ). Systemic acquired silencing: transgene-specific post-transcriptional silencing is transmitted by grafting from silenced stocks to non-silenced scions. EMBO J 16, 4738–4745.[CrossRef]
    [Google Scholar]
  29. Riechmann, J. L., Lain, S. & García, J. A. ( 1992; ). Highlights and prospects of potyvirus molecular biology. J Gen Virol 73, 1–16.[CrossRef]
    [Google Scholar]
  30. Roth, B. M., Pruss, G. J. & Vance, V. B. ( 2004; ). Plant viral suppressors of RNA silencing. Virus Res 102, 97–108.[CrossRef]
    [Google Scholar]
  31. Ryang, B.-S., Kobori, T., Matsumoto, T., Kosaka, Y. & Ohki, S. T. ( 2004; ). Cucumber mosaic virus 2b protein compensates for restricted systemic spread of Potato virus Y in doubly infected tobacco. J Gen Virol 85, 3405–3414.[CrossRef]
    [Google Scholar]
  32. Saiga, T., Fujiwara, M., Saitoh, H., Ohki, S. T. & Osaki, T. ( 1998; ). Comparative analysis for replication and movement of cucumber mosaic virus in Cucumis figarei and C. melo. Ann Phytopathol Soc Jpn 64, 255–263.[CrossRef]
    [Google Scholar]
  33. Sato, K., Yoshikawa, N. & Takahashi, T. ( 1993; ). Complete nucleotide sequence of the genome of an apple isolate of apple chlorotic leaf spot virus. J Gen Virol 74, 1927–1931.[CrossRef]
    [Google Scholar]
  34. Satoh, H., Yoshikawa, N. & Takahashi, T. ( 1999; ). Construction and biolistic inoculation of an infectious cDNA clone of apple chlorotic leaf spot trichovirus. Ann Phytopathol Soc Jpn 65, 301–304.[CrossRef]
    [Google Scholar]
  35. Satoh, H., Matsuda, H., Kawamura, T., Isogai, M., Yoshikawa, N. & Takahashi, T. ( 2000; ). Intracellular distribution, cell-to-cell trafficking and tubule-inducing activity of the 50 kDa movement protein of Apple chlorotic leaf spot virus fused to green fluorescent protein. J Gen Virol 81, 2085–2093.
    [Google Scholar]
  36. Takahashi, Y., Takahashi, T. & Uyeda, I. ( 1997; ). A cDNA clone to clover yellow vein potyvirus genome is highly infectious. Virus Genes 14, 235–243.[CrossRef]
    [Google Scholar]
  37. Vance, V. & Vaucheret, H. ( 2001; ). RNA silencing in plants - defense and counterdefense. Science 292, 2277–2280.[CrossRef]
    [Google Scholar]
  38. Voinnet, O. ( 2005a; ). Induction and suppression of RNA silencing: insights from viral infections. Nat Rev Genet 6, 206–220.[CrossRef]
    [Google Scholar]
  39. Voinnet, O. ( 2005b; ). Non-cell autonomous RNA silencing. FEBS Lett 579, 5858–5871.[CrossRef]
    [Google Scholar]
  40. Voinnet, O. & Baulcombe, D. C. ( 1997; ). Systemic signalling in gene silencing. Nature 389, 553.[CrossRef]
    [Google Scholar]
  41. Voinnet, O., Vain, P., Angell, S. & Baulcombe, D. C. ( 1998; ). Systemic spread of sequence-specific transgene RNA degradation in plants is initiated by localized introduction of ectopic promoterless DNA. Cell 95, 177–187.[CrossRef]
    [Google Scholar]
  42. Voinnet, O., Lederer, C. & Baulcombe, D. C. ( 2000; ). A viral movement protein prevents spread of the gene silencing signal in Nicotiana benthamiana. Cell 103, 157–167.[CrossRef]
    [Google Scholar]
  43. Voinnet, O., Rivas, S., Mestre, P. & Baulcombe, D. C. ( 2003; ). An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus. Plant J 33, 949–956.[CrossRef]
    [Google Scholar]
  44. Wang, M. B. & Metzlaff, M. ( 2005; ). RNA silencing and antiviral defense in plants. Curr Opin Plant Biol 8, 216–222.[CrossRef]
    [Google Scholar]
  45. Yanase, H. ( 1974; ). Studies on apple latent viruses in Japan. Bull Fruit Tree Res Sn Jpn Ser C1, 47–109.
    [Google Scholar]
  46. Yoshikawa, N. & Takahashi, T. ( 1988; ). Properties of RNAs and proteins of apple stem grooving and apple chlorotic leaf spot viruses. J Gen Virol 69, 241–245.[CrossRef]
    [Google Scholar]
  47. Yoshikawa, N., Oogake, S., Terada, M., Miyabayashi, S., Ikeda, Y., Takahashi, T. & Ogawa, K. ( 1999; ). Apple chlorotic leaf spot virus 50 kDa protein is targeted to plasmodesmata and accumulates in sieve elements in transgenic plant leaves. Arch Virol 144, 2475–2483.[CrossRef]
    [Google Scholar]
  48. Yoshikawa, N., Gotoh, S., Umezawa, M., Satoh, N., Satoh, H., Takahashi, T., Ito, T. & Yoshida, K. ( 2000; ). Transgenic Nicotiana occidentalis plants expressing the 50-kDa protein of Apple chlorotic leaf spot virus display increased susceptibility to homologous virus, but strong resistance to Grapevine berry inner necrosis virus. Phytopathology 90, 311–316.[CrossRef]
    [Google Scholar]
  49. Yoshikawa, N., Saitou, Y., Kitajima, A., Chida, T., Sasaki, N. & Isogai, M. ( 2006; ). Interference of long-distance movement of Grapevine berry inner necrosis virus in transgenic plants expressing a defective movement protein of Apple chlorotic leaf spot virus. Phytopathology 96, 378–385.[CrossRef]
    [Google Scholar]
  50. Zamore, P. D. ( 2002; ). Ancient pathways programmed by small RNAs. Science 296, 1265–1269.[CrossRef]
    [Google Scholar]
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